| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,106,741 | A→T | S162S (TCT→TCA) | wbbH ← | putative O‑antigen polymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,106,741 | 0 | A | T | 85.7% | 14.5 / ‑5.5 | 7 | coding (486/1167 nt) | wbbH | putative O‑antigen polymerase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (4/2); minor base . (1/0); total (5/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGT > NC_000913/2106627‑2106860 | aatataaaCCAAAAAGAAAATTTGTTTACCAGTTTTCAG‑AAAAATGCAAAAATGGAAACAAAAAAAACAAGTAAAGGAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAAtaaataaa < 1:129143/123‑1 (MQ=255) ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATCCAATAAAAAAAAGTAAGGTAAATGTTTTACTTACCTTGTTATTAGAAAATTTTTT‑TACCAAAAAAAAAAACCaaaaa > 1:46128/1‑127 (MQ=255) aaattcAAAAATGTAAACAAAAAAAACCAGGAAAGTAAAAGTTGTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTgaga < 1:384541/123‑1 (MQ=255) aaaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCCAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTAAACAt > 1:167777/1‑129 (MQ=255) cTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCt > 1:591650/1‑129 (MQ=255) tAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCAGGTATGCTGAGAAATTTCTTGATGTGTCTTCAAAATCGGAATCCCTTTTCAAATTCATTAAGTtaagaaaga > 1:458156/1‑128 (MQ=255) tAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGGGAAATTTCTTGATGTGTTTTCACCATAAGAATCCTTTTTAAAATTCAT‑TAGCCTAGTTAGCTAGt > 1:256944/1‑129 (MQ=255) | GATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGT > NC_000913/2106627‑2106860 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |