| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,544,022 | 1 bp→AA | coding (39/1389 nt) | narU ← | nitrate/nitrite transporter NarU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,544,021 | 1 | . | A | 75.0% | 12.4 / ‑3.2 | 8 | coding (40/1389 nt) | narU | nitrate/nitrite transporter NarU |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (1/5); total (2/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 1,544,022 | 0 | C | A | 87.5% | 15.0 / NA | 8 | L13F (TTG→TTT) | narU | nitrate/nitrite transporter NarU |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (1/6); total (2/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑G‑CAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAG > NC_000913/1543901‑1544135 || tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACACCTAACTGAAATCCAGAGATTTCTTCGAGCAATATGGTTTCCTTTATTTTCCCAAAGGGCCGGATTTTCTGTTTCCCAGTCGC‑G‑Caaaaaga > 1:527973/1‑126 (MQ=255) ctgaatgaTATCCAGGGATTTCTTCGAGCAATTTGTTGTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt < 1:228134/129‑1 (MQ=255) ttcttcGAGAAAATTGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTgg < 1:504097/129‑1 (MQ=255) tttCCTTTTTTTTCCCCAAAGG‑CGGGTTTTCGTGGTTCCAGTCGC‑GAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaaa < 1:7369/129‑1 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGGTTCCAGTCGC‑GAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAaca < 1:594688/128‑1 (MQ=255) aGAAGGCCGTATTTTTTGGTTTCCAGTCGC‑GAAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc < 1:562668/128‑1 (MQ=255) aGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑GAAAAAAGATAACGACTATTTTTCTCATTTTTCAGTGCCCAATTGTTCCCCACATGCACACCTTGGTAAT‑AAAAAAAGACAAAAAACGGGGaaaag > 1:199294/1‑124 (MQ=255) ggTGTCCAGTCGCAA‑AAAAAGATACCGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCGCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAg < 1:501635/129‑1 (MQ=255) || TAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGC‑G‑CAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAG > NC_000913/1543901‑1544135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |