BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	506.4 / NA	123	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (57/66); total (57/66)

Only 100 of 123 total aligned reads displayed.

TGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742709‑1742801

tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                             <  1:3900334/50‑1 (MQ=255)

  cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            <  1:622462/49‑1 (MQ=255)

   gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           >  1:4482304/1‑49 (MQ=255)

   gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:4441633/50‑1 (MQ=255)

    ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                         <  1:7909976/50‑1 (MQ=255)

     cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:1200354/1‑50 (MQ=255)

     cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        <  1:7628728/50‑1 (MQ=255)

     cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:7029011/1‑50 (MQ=255)

     cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:1631599/1‑50 (MQ=255)

     cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:5868116/1‑50 (MQ=255)

      aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       >  1:606362/1‑50 (MQ=255)

        gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                     <  1:3335699/50‑1 (MQ=255)

        gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                     <  1:7336748/50‑1 (MQ=255)

        gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:7032507/1‑49 (MQ=255)

        gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:5495158/1‑49 (MQ=255)

        gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:3342436/1‑49 (MQ=255)

        gTTTTTTCTTCCCAAGAGTAGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                     <  1:1782740/50‑1 (MQ=255)

         ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:4793458/1‑50 (MQ=255)

         ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    <  1:1334422/50‑1 (MQ=255)

         ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:4980601/1‑50 (MQ=255)

           ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:2513688/49‑1 (MQ=255)

           ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:377372/1‑50 (MQ=255)

            tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:3314062/1‑49 (MQ=255)

              tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                >  1:6526758/1‑49 (MQ=255)

              tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:4699468/49‑1 (MQ=255)

              tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:7751382/49‑1 (MQ=255)

              tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                >  1:4009602/1‑49 (MQ=255)

              tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:3122449/49‑1 (MQ=255)

               cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               >  1:1900150/1‑49 (MQ=255)

               cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               >  1:6678249/1‑49 (MQ=255)

               cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               <  1:3845194/49‑1 (MQ=255)

                ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              >  1:5194693/1‑49 (MQ=255)

                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            <  1:1996446/49‑1 (MQ=255)

                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            <  1:4349667/49‑1 (MQ=255)

                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           >  1:2482582/1‑50 (MQ=255)

                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           >  1:6449884/1‑50 (MQ=255)

                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                          <  1:8033611/50‑1 (MQ=255)

                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                          <  1:7931037/50‑1 (MQ=255)

                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:344607/49‑1 (MQ=255)

                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:7442307/49‑1 (MQ=255)

                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:8293638/49‑1 (MQ=255)

                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:4817949/1‑50 (MQ=255)

                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         <  1:7160295/50‑1 (MQ=255)

                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         <  1:5718229/50‑1 (MQ=255)

                     aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        >  1:7808060/1‑50 (MQ=255)

                      agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                       >  1:4025587/1‑50 (MQ=255)

                       gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                      >  1:1974644/1‑50 (MQ=255)

                        agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     >  1:6931791/1‑50 (MQ=255)

                        agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:5329955/50‑1 (MQ=255)

                        agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:4358995/50‑1 (MQ=255)

                        agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     >  1:1684777/1‑50 (MQ=255)

                        agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:2767900/50‑1 (MQ=255)

                        agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     >  1:2981539/1‑50 (MQ=255)

                         gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    <  1:1469798/50‑1 (MQ=255)

                         gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    >  1:7299318/1‑50 (MQ=255)

                          tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                   >  1:2345401/1‑50 (MQ=255)

                          tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                   >  1:3451292/1‑50 (MQ=255)

                           cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:4672823/1‑50 (MQ=255)

                           cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:406694/1‑50 (MQ=255)

                            gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  <  1:7077280/49‑1 (MQ=255)

                            gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  <  1:7252493/49‑1 (MQ=255)

                            gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:7541349/1‑50 (MQ=255)

                             tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:5279120/1‑49 (MQ=255)

                             tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg                <  1:7631133/50‑1 (MQ=255)

                             tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg                <  1:4178144/50‑1 (MQ=255)

                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa               <  1:5609833/49‑1 (MQ=255)

                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa               <  1:7964916/49‑1 (MQ=255)

                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa               <  1:5555733/49‑1 (MQ=255)

                                gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             >  1:1539498/1‑50 (MQ=255)

                                gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             >  1:8303985/1‑50 (MQ=255)

                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            >  1:3392203/1‑50 (MQ=255)

                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            <  1:5384289/50‑1 (MQ=255)

                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            >  1:862483/1‑50 (MQ=255)

                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            <  1:3776887/50‑1 (MQ=255)

                                  tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           >  1:4982320/1‑50 (MQ=255)

                                   ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:5158127/49‑1 (MQ=255)

                                   ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:678724/49‑1 (MQ=255)

                                   ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:5963476/49‑1 (MQ=255)

                                   ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:5881785/49‑1 (MQ=255)

                                   ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:5383223/49‑1 (MQ=255)

                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg          <  1:4596798/49‑1 (MQ=255)

                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         >  1:1240877/1‑50 (MQ=255)

                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         <  1:1482055/50‑1 (MQ=255)

                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:3487384/1‑50 (MQ=255)

                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        <  1:1991750/50‑1 (MQ=255)

                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        <  1:4712318/50‑1 (MQ=255)

                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:491248/1‑50 (MQ=255)

                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        <  1:5167963/50‑1 (MQ=255)

                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:5529844/1‑50 (MQ=255)

                                      gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg       <  1:4020812/50‑1 (MQ=255)

                                      gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg       <  1:3244420/50‑1 (MQ=255)

                                       tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      <  1:5944319/50‑1 (MQ=255)

                                        gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:3301975/1‑50 (MQ=255)

                                        gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:6484135/1‑50 (MQ=255)

                                         tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     <  1:3021978/49‑1 (MQ=255)

                                         tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:5151250/50‑1 (MQ=255)

                                         tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:1959486/50‑1 (MQ=255)

                                         tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:8334766/1‑50 (MQ=255)

                                          ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   >  1:2392363/1‑50 (MQ=255)

                                           tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt  >  1:6905014/1‑50 (MQ=255)

TGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742709‑1742801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`