BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	531.8 / NA	128	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (52/76); total (52/76)

Only 100 of 130 total aligned reads displayed.

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:3260711/49‑1 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:2972757/49‑1 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  1:1168724/1‑50 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  1:2214756/1‑50 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:1282041/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  1:5178292/50‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:8033802/1‑50 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:7840061/1‑50 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                           <  1:7250522/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:5417696/1‑48 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:7100288/1‑48 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:2115002/1‑48 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:6452019/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:2498420/50‑1 (MQ=255)

     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:2108048/1‑49 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:187744/49‑1 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       <  1:5791367/50‑1 (MQ=255)

       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:576018/1‑49 (MQ=255)

        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:3861058/1‑50 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:3522163/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:4752057/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:6435918/49‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:414986/50‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  1:866315/49‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  1:8127465/49‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                 <  1:5478233/50‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                 <  1:1524244/50‑1 (MQ=255)

             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                >  1:4253429/1‑50 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:7613368/1‑50 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               <  1:4993628/50‑1 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               <  1:4458610/50‑1 (MQ=255)

               tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                              <  1:5952139/50‑1 (MQ=255)

               tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                              >  1:3825977/1‑50 (MQ=255)

                cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:4615375/1‑50 (MQ=255)

                cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:2783710/1‑50 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:6746106/1‑49 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:1729274/1‑49 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:2613586/1‑49 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  1:6864268/47‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  1:2301358/47‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:6012454/48‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:7260171/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:5080354/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:7433187/50‑1 (MQ=255)

                    tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:5963562/1‑49 (MQ=255)

                    tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:7912974/1‑49 (MQ=255)

                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  1:5717150/1‑50 (MQ=255)

                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  1:1491300/1‑50 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:737626/1‑50 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:5014169/1‑50 (MQ=255)

                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:6117891/1‑50 (MQ=255)

                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:5394354/1‑49 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  1:1883189/44‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:5795763/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:1882297/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:5729102/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:2425626/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:6951990/47‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:3407751/47‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:5003723/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:7562575/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:7261478/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:6469547/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:3381036/48‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 <  1:910809/48‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 <  1:6813327/48‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:2977844/49‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  1:6052873/1‑49 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:7620642/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:7025016/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:2633047/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:6180019/50‑1 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             <  1:5657342/50‑1 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             >  1:5470221/1‑50 (MQ=255)

                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  1:6087741/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:2067417/49‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:2255124/49‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:5259131/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:90094/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:7255115/50‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:2704547/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:6087654/50‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:3821885/50‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:6136023/50‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:3007910/50‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:4129985/50‑1 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCGGCTGa         <  1:7829607/50‑1 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  1:8062384/50‑1 (MQ=255)

                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        <  1:7629178/50‑1 (MQ=255)

                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       <  1:8093238/50‑1 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:6501743/1‑50 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:4734386/50‑1 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:3617433/50‑1 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:1686428/1‑50 (MQ=255)

                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     <  1:4327936/50‑1 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:1284173/49‑1 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:2547452/49‑1 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   >  1:2001008/1‑50 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   <  1:6336834/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:4632686/1‑50 (MQ=255)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`