Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,513,003 A→G D212D (GAT→GAC)  trpS ← tryptophan‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,513,0030AG100.0% 510.7 / NA 125D212D (GAT→GAC) trpStryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (75/50);  total (75/50)

Only 100 of 125 total aligned reads displayed.

TCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512956‑3513048
                                               |                                             
tcgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                             <  1:8270630/50‑1 (MQ=255)
tcgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                             <  1:845832/50‑1 (MQ=255)
 cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:875251/1‑50 (MQ=255)
 cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:5941152/1‑50 (MQ=255)
 cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:6359913/1‑50 (MQ=255)
 cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:7755543/1‑50 (MQ=255)
  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  1:3495545/1‑50 (MQ=255)
   gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          <  1:696527/50‑1 (MQ=255)
   gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          <  1:7302137/50‑1 (MQ=255)
   gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:1540654/1‑50 (MQ=255)
    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:1868409/50‑1 (MQ=255)
     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:6227309/50‑1 (MQ=255)
      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       <  1:1606267/50‑1 (MQ=255)
      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       <  1:3990414/50‑1 (MQ=255)
       cAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:208125/1‑49 (MQ=255)
       cAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:6387832/1‑49 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:1553922/50‑1 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:5704062/49‑1 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:5078690/1‑48 (MQ=255)
        aGTGACCGCACGTTTGATTTTATTCACTACCGATTTCGGGTCTTCcagca                                     <  1:6999445/50‑1 (MQ=255)
         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:2085614/1‑50 (MQ=255)
          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:5454199/1‑50 (MQ=255)
          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:2370371/50‑1 (MQ=255)
          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:7575719/1‑50 (MQ=255)
          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:3834793/50‑1 (MQ=255)
          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:4163444/1‑50 (MQ=255)
           gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:3292035/1‑50 (MQ=255)
           gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:2595668/1‑50 (MQ=255)
           gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:7137703/1‑50 (MQ=255)
           gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:6774511/1‑50 (MQ=255)
            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:5001328/1‑50 (MQ=255)
            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:3991487/50‑1 (MQ=255)
            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:7048598/50‑1 (MQ=255)
            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  <  1:157922/49‑1 (MQ=255)
             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1663802/1‑49 (MQ=255)
             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:5660538/1‑49 (MQ=255)
             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:8049978/1‑49 (MQ=255)
             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCg                                <  1:2666287/50‑1 (MQ=255)
              cGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               <  1:356629/50‑1 (MQ=255)
              cGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               <  1:3734248/50‑1 (MQ=255)
              cGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:2179004/1‑50 (MQ=255)
               gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:4565835/1‑49 (MQ=255)
               gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              <  1:59296/50‑1 (MQ=255)
                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:2927438/1‑49 (MQ=255)
                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:6539110/1‑49 (MQ=255)
                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:8094606/50‑1 (MQ=255)
                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:6918526/50‑1 (MQ=255)
                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           >  1:3190423/1‑50 (MQ=255)
                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:4565450/1‑50 (MQ=255)
                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:4569652/1‑50 (MQ=255)
                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          <  1:404727/50‑1 (MQ=255)
                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:290197/1‑50 (MQ=255)
                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         <  1:2758199/50‑1 (MQ=255)
                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:3206568/1‑50 (MQ=255)
                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:5636080/1‑50 (MQ=255)
                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:7527428/1‑50 (MQ=255)
                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       <  1:3550345/49‑1 (MQ=255)
                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       <  1:1138802/49‑1 (MQ=255)
                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:672380/1‑50 (MQ=255)
                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:5206817/1‑50 (MQ=255)
                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     <  1:7181182/50‑1 (MQ=255)
                        aTTTTCTTCACTACCGATTTCGGGACTTCCAGCAGCCGGATAACGTTAtt                     >  1:6224380/1‑50 (MQ=37)
                           ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:5249133/1‑49 (MQ=255)
                           ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   <  1:2327033/49‑1 (MQ=255)
                            tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg                  <  1:1062358/49‑1 (MQ=255)
                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:52335/1‑50 (MQ=255)
                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:2774044/1‑50 (MQ=255)
                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:16207/1‑50 (MQ=255)
                              ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:5109899/1‑49 (MQ=255)
                              ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:2472204/1‑49 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              <  1:774016/49‑1 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:4527746/1‑50 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:854008/1‑50 (MQ=255)
                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             <  1:4153825/49‑1 (MQ=255)
                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            >  1:1869748/1‑50 (MQ=255)
                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:6454684/50‑1 (MQ=255)
                                  cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           <  1:2115132/50‑1 (MQ=255)
                                   tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:751795/1‑50 (MQ=255)
                                   tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:4138785/1‑50 (MQ=255)
                                    aCCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2309832/1‑49 (MQ=255)
                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2651990/1‑48 (MQ=255)
                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:7322788/48‑1 (MQ=255)
                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:4622892/49‑1 (MQ=255)
                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:5299494/49‑1 (MQ=255)
                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:3600523/1‑47 (MQ=255)
                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:4019108/1‑47 (MQ=255)
                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:1027124/1‑47 (MQ=255)
                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAg       <  1:5321950/50‑1 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:5603970/1‑50 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:924560/1‑50 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:2403660/50‑1 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:4428342/1‑50 (MQ=255)
                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:7825678/1‑50 (MQ=255)
                                         tttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt    >  1:3167794/1‑50 (MQ=255)
                                         tttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt    <  1:4211763/50‑1 (MQ=255)
                                         tttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt    <  1:5540133/50‑1 (MQ=255)
                                           tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   <  1:882113/49‑1 (MQ=255)
                                           tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:1708304/1‑50 (MQ=255)
                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:7747686/1‑49 (MQ=255)
                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:7542936/1‑49 (MQ=255)
                                               |                                             
TCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512956‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑