BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	485.1 / NA	119	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (54/65); total (54/65)

Only 100 of 119 total aligned reads displayed.

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438077‑4438168

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                            >  1:6579759/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                           <  1:164661/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          >  1:2460468/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          >  1:3618416/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          <  1:7133519/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          >  1:8175792/1‑49 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          >  1:1506208/1‑49 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                        <  1:8052746/50‑1 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                        >  1:1080287/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                       <  1:7177666/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                       >  1:5730233/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                       <  1:2163272/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                       <  1:2236498/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                      <  1:4684587/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                      >  1:1070820/1‑50 (MQ=255)

       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                     <  1:5659921/50‑1 (MQ=255)

       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                     >  1:5245132/1‑50 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                     >  1:1319381/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                    <  1:5204985/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   <  1:4409311/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   <  1:1903536/50‑1 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   >  1:2722775/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                  <  1:8335175/50‑1 (MQ=255)

           gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   >  1:3714340/1‑48 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   >  1:2222055/1‑47 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                 <  1:633731/49‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                <  1:3632915/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                              <  1:4674064/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                              <  1:8233379/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                               >  1:5514715/1‑49 (MQ=255)

                tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                             >  1:75603/1‑49 (MQ=255)

                tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                             >  1:1340291/1‑49 (MQ=255)

                tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                             >  1:6751910/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                            >  1:3280535/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                            >  1:6952733/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                            <  1:3833562/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          <  1:7427037/50‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          <  1:4583300/50‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                           <  1:5506414/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                           <  1:8332480/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                           <  1:5221945/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                           <  1:6636403/49‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:7361648/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:4577859/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:6068071/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:3171169/1‑49 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:4105467/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:4762614/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:5212494/1‑48 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                       >  1:6289247/1‑50 (MQ=255)

                      aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                       <  1:7869447/49‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                      <  1:1704002/49‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                      <  1:7157209/49‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                     <  1:4222633/50‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                     <  1:6171086/50‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                     >  1:37983/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                     >  1:165877/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                     >  1:7822413/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                    <  1:1911914/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                    <  1:6064357/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                    <  1:2735568/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                    <  1:4097718/50‑1 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                    >  1:7907490/1‑49 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                   <  1:3147372/50‑1 (MQ=255)

                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                  >  1:6062216/1‑50 (MQ=255)

                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                  <  1:6185896/50‑1 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                  >  1:5584321/1‑49 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                  >  1:1335062/1‑49 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                 <  1:2079379/50‑1 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                 <  1:7458657/50‑1 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                 <  1:201947/50‑1 (MQ=255)

                            aaaCAGACGATTGTATGCTCGGGATATTCTACAGAAAAAACAGTCTGACa                >  1:7492854/1‑50 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                <  1:7906811/50‑1 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                <  1:1040171/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt               <  1:1342396/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt               <  1:3081852/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt               <  1:1252895/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa              <  1:4973650/50‑1 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc             <  1:3541743/50‑1 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc             >  1:3486410/1‑50 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc             <  1:46258/50‑1 (MQ=255)

                                aGACGTTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            >  1:6546875/1‑50 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            <  1:5409205/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            <  1:1826825/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            >  1:1422535/1‑50 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            <  1:5916026/50‑1 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            >  1:1054990/1‑49 (MQ=255)

                                  aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa          <  1:666972/50‑1 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa         >  1:1157567/1‑49 (MQ=255)

                                       tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      >  1:6066913/1‑49 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg     <  1:381426/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa    <  1:6276466/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   >  1:7698980/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   >  1:4575715/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   <  1:7462157/50‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   >  1:1774783/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   <  1:8306082/50‑1 (MQ=255)

                                          aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   >  1:2951264/1‑49 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt  >  1:3191258/1‑49 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt  >  1:5700347/1‑49 (MQ=255)

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438077‑4438168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`