Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	354.7 / NA	92	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/57);  total (35/57)

TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382508‑1382601                                                |                                               ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              >  1:7068186/1‑50 (MQ=255)  tGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              >  1:3295610/1‑49 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              <  1:5137825/48‑1 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                             >  1:5203819/1‑49 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCa                                            <  1:7186444/49‑1 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:4926285/1‑49 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:7597276/50‑1 (MQ=255)      aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          >  1:5454368/1‑49 (MQ=255)      aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          >  1:142857/1‑49 (MQ=255)      aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:2659598/50‑1 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:6500665/50‑1 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       >  1:3051574/1‑50 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:7560818/50‑1 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:2197886/50‑1 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:69794/49‑1 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:2140669/49‑1 (MQ=255)           aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:6107929/1‑50 (MQ=255)            cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                   <  1:2079622/50‑1 (MQ=255)            cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                   <  1:7831709/50‑1 (MQ=255)             tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:7621/1‑48 (MQ=255)             tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:7830435/1‑48 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGa                                  <  1:6358224/48‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGa                                  <  1:6664611/48‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 <  1:3970811/49‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                >  1:2336802/1‑50 (MQ=255)                tatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               >  1:6517702/1‑50 (MQ=255)                 atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              >  1:4886954/1‑50 (MQ=255)                 atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               <  1:1708815/49‑1 (MQ=255)                 atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               <  1:1426657/49‑1 (MQ=255)                  tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              <  1:6315995/49‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:244871/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:7806698/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:1715330/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:4517565/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          <  1:1942008/49‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          <  1:6033846/49‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:1551582/50‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:532233/50‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:808170/50‑1 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        >  1:6581747/1‑50 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        >  1:1116187/1‑50 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        >  1:5365159/1‑50 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        >  1:7395433/1‑50 (MQ=255)                        gcgcGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        >  1:1509938/1‑49 (MQ=255)                         cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCa                      <  1:5161433/50‑1 (MQ=255)                          gcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:866158/1‑48 (MQ=255)                           cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:7774585/50‑1 (MQ=255)                           cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:5360114/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    >  1:2721277/1‑49 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:3583071/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:4316276/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:6401081/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:3851584/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:6604639/50‑1 (MQ=255)                             gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   >  1:3833292/1‑49 (MQ=255)                             gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   >  1:3025660/1‑49 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:5376282/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 >  1:6034436/1‑50 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:4611761/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTACACGCAAGaaa                 >  1:7146973/1‑50 (MQ=255)                               ttCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                >  1:3767657/1‑50 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:4624729/49‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:4274676/48‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:3510220/48‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              >  1:3507638/1‑50 (MQ=255)                                  aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:5886291/49‑1 (MQ=255)                                  aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:5472428/49‑1 (MQ=255)                                    tatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgcg           <  1:4404790/50‑1 (MQ=255)                                    tatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgcg           <  1:2088974/50‑1 (MQ=255)                                     atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:3480590/1‑50 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          <  1:139409/49‑1 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          <  1:325137/49‑1 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:1895166/1‑50 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         <  1:3920918/50‑1 (MQ=255)                                       ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:236796/1‑49 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt        <  1:2774578/49‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       >  1:1221520/1‑50 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       <  1:1588875/50‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       <  1:2341885/50‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       <  1:5161363/50‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       >  1:6637103/1‑50 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      >  1:6671385/1‑50 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      >  1:5884641/1‑50 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:6486691/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:1823897/50‑1 (MQ=255)                                           gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:5550465/50‑1 (MQ=255)                                           gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:5885644/50‑1 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   >  1:2636852/1‑50 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:250918/50‑1 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   >  1:3649394/1‑50 (MQ=255)                                             cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:7173112/50‑1 (MQ=255)                                             cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:789175/50‑1 (MQ=255)                                                |                                               TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382508‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑