Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	392.7 / NA	97	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (55/42);  total (55/42)

GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801                                                |                                               gTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGa                                              <  1:7458863/50‑1 (MQ=255)  tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                             >  1:983544/1‑50 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           <  1:2815341/50‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           <  1:6598490/50‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:1439780/1‑49 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:6155123/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           >  1:1792778/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:2366001/50‑1 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:8282794/50‑1 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:3330075/50‑1 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:7404891/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:2542613/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:3765489/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:4005960/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        <  1:4187938/50‑1 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       <  1:1287427/50‑1 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       <  1:6424416/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:6405706/1‑50 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:6174401/1‑50 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                     <  1:7499829/50‑1 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:3806593/1‑49 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    <  1:7573536/50‑1 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:1294284/1‑50 (MQ=255)            tttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   >  1:6170580/1‑50 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:7723670/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:7910725/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:5853224/1‑50 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  <  1:4751553/49‑1 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  <  1:8161443/49‑1 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:4385495/1‑49 (MQ=255)              tttCTTCCCAAGAGTCGGGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:7875526/1‑49 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                 >  1:832721/1‑49 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                 <  1:8303488/49‑1 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                 >  1:6278750/1‑49 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:3144527/49‑1 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:2003977/49‑1 (MQ=255)                  ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              >  1:1666882/1‑49 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                             <  1:7535826/48‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           >  1:1826992/1‑50 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                          >  1:1949540/1‑50 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:6344356/49‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:2039601/49‑1 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:1946657/1‑50 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:1984933/1‑50 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:500394/1‑50 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        >  1:4304883/1‑50 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        <  1:2138280/50‑1 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        >  1:1349988/1‑50 (MQ=255)                        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                       <  1:7513499/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                      <  1:3127090/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:2681568/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     >  1:5909549/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    >  1:6470981/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    >  1:1138600/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    >  1:1470219/1‑50 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                   >  1:5837834/1‑50 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                   <  1:427620/50‑1 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:1698075/1‑50 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:7888766/1‑50 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:4741990/1‑50 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:4937466/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  <  1:8320996/49‑1 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:7725628/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:7704871/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:656777/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:4698119/1‑50 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg                <  1:948307/50‑1 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg                <  1:2338866/50‑1 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              >  1:2826933/1‑50 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              >  1:1809759/1‑50 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:4675258/50‑1 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:511240/50‑1 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:6730397/50‑1 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:8018633/50‑1 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:2181364/50‑1 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            >  1:815865/1‑50 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            <  1:8303338/50‑1 (MQ=255)                                    tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           >  1:6678879/1‑50 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:448380/49‑1 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         >  1:1587067/1‑50 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:2002771/1‑50 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:636664/1‑50 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        <  1:2646632/50‑1 (MQ=255)                                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:2722188/1‑49 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      >  1:7038423/1‑50 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      >  1:1140702/1‑50 (MQ=255)                                          gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:8227472/1‑50 (MQ=255)                                          gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:1094325/1‑50 (MQ=255)                                          gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:4970265/1‑50 (MQ=255)                                          gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:5218486/1‑50 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     <  1:426205/49‑1 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     <  1:6580077/49‑1 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:4206186/50‑1 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:6687213/50‑1 (MQ=255)                                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   >  1:6155312/1‑50 (MQ=255)                                             tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   <  1:7664362/49‑1 (MQ=255)                                             tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt  >  1:883399/1‑50 (MQ=255)                                                |                                               GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑