| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 257,794 | T→C | intergenic (+23/‑35) | frsA → / → crl | fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 257,794 | 0 | T | C | 99.4% | 666.1 / NA | 160 | intergenic (+23/‑35) | frsA/crl | fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (86/73); minor base A (1/0); total (87/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 160 total aligned reads displayed.
CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG > NC_000913/257747‑257840 | cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg < 1:545320/50‑1 (MQ=255)cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg < 1:3415787/50‑1 (MQ=255)cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg < 1:5966304/50‑1 (MQ=255)cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg < 1:2040263/50‑1 (MQ=255) gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg < 1:248618/48‑1 (MQ=255) gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt > 1:1134477/1‑49 (MQ=255) aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa < 1:4093152/50‑1 (MQ=255) aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa < 1:4171306/50‑1 (MQ=255) aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa > 1:7177737/1‑49 (MQ=255) tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa < 1:7773814/50‑1 (MQ=255) tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa < 1:4638777/50‑1 (MQ=255) tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa > 1:5306406/1‑49 (MQ=255) tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa > 1:6965145/1‑49 (MQ=255) tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa > 1:3206921/1‑49 (MQ=255) gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa > 1:8051451/1‑49 (MQ=255) gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa < 1:5037581/49‑1 (MQ=255) gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa < 1:5423813/49‑1 (MQ=255) gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc < 1:2718219/50‑1 (MQ=255) aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa > 1:7461038/1‑48 (MQ=255) aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa > 1:4158963/1‑48 (MQ=255) aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa < 1:3124036/50‑1 (MQ=255) aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa < 1:4536261/50‑1 (MQ=255) aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa < 1:47474/50‑1 (MQ=255) aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg < 1:3811688/50‑1 (MQ=255) aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt < 1:1302122/50‑1 (MQ=255) aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt < 1:5563440/50‑1 (MQ=255) aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt < 1:4775043/50‑1 (MQ=255) aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt < 1:7364531/50‑1 (MQ=255) aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg > 1:955617/1‑50 (MQ=255) aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg < 1:2516595/50‑1 (MQ=255) aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg < 1:5965797/50‑1 (MQ=255) cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATATGGTAAAACAGTTg > 1:2192756/1‑49 (MQ=255) gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa > 1:7980062/1‑50 (MQ=255) cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt > 1:4321045/1‑50 (MQ=255) cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt > 1:4256244/1‑50 (MQ=255) cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt > 1:7116069/1‑50 (MQ=255) ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt > 1:2984716/1‑49 (MQ=255) ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt > 1:5683046/1‑49 (MQ=255) gtgtTCAACATTTGCTCCATTTTGCCAATCTTGTAAAACAGTTGCATca > 1:6148602/1‑49 (MQ=255) gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca < 1:1735460/49‑1 (MQ=255) tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca < 1:4442285/50‑1 (MQ=255) tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac > 1:7357726/1‑49 (MQ=255) tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac > 1:1771189/1‑49 (MQ=255) tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa > 1:6444506/1‑48 (MQ=255) aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca < 1:5616991/49‑1 (MQ=255) aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca < 1:2396651/49‑1 (MQ=255) aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg > 1:2162935/1‑50 (MQ=255) aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAgg < 1:4475753/50‑1 (MQ=255) aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg > 1:5879710/1‑49 (MQ=255) aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg < 1:3947978/49‑1 (MQ=255) aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg > 1:4612777/1‑48 (MQ=255) aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag < 1:5120067/50‑1 (MQ=255) aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg > 1:6467158/1‑46 (MQ=255) tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt < 1:3012761/50‑1 (MQ=255) tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt < 1:2671722/50‑1 (MQ=255) tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa < 1:7672726/49‑1 (MQ=255) tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa < 1:3381855/49‑1 (MQ=255) gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg > 1:5014488/1‑49 (MQ=255) cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc > 1:5507729/1‑49 (MQ=255) cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc < 1:5356059/49‑1 (MQ=255) cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc > 1:6954530/1‑49 (MQ=255) cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc > 1:578897/1‑49 (MQ=255) cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCa < 1:6436285/50‑1 (MQ=255) tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa > 1:6191537/1‑50 (MQ=255) aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt > 1:4123606/1‑50 (MQ=255) aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt > 1:192462/1‑50 (MQ=255) aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt > 1:1405414/1‑50 (MQ=255) aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt > 1:3890530/1‑50 (MQ=255) aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa < 1:1058411/48‑1 (MQ=255) aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt > 1:4842863/1‑49 (MQ=255) aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt < 1:2764316/49‑1 (MQ=255) aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt > 1:6752797/1‑49 (MQ=255) aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt > 1:8185204/1‑49 (MQ=255) aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg < 1:6711788/50‑1 (MQ=255) aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg < 1:6787255/50‑1 (MQ=255) aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa > 1:3943467/1‑50 (MQ=255) aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa > 1:6302199/1‑50 (MQ=255) aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa < 1:7576611/50‑1 (MQ=255) aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa > 1:573090/1‑50 (MQ=255) aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa < 1:3886875/50‑1 (MQ=255) ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc > 1:5416295/1‑50 (MQ=255) ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc > 1:826574/1‑50 (MQ=255) ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc > 1:4044043/1‑50 (MQ=255) ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc < 1:6807531/50‑1 (MQ=255) tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg > 1:101380/1‑50 (MQ=255) tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg > 1:7299761/1‑50 (MQ=255) tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt > 1:4237134/1‑49 (MQ=255) tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt < 1:2093149/49‑1 (MQ=255) tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt > 1:8088799/1‑49 (MQ=255) gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt < 1:2345701/49‑1 (MQ=255) gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa > 1:750938/1‑50 (MQ=255) ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa < 1:2242054/49‑1 (MQ=255) ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa < 1:115765/49‑1 (MQ=255) ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc > 1:3247934/1‑50 (MQ=255) ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc > 1:6933505/1‑50 (MQ=255) ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc > 1:7034316/1‑50 (MQ=255) cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc > 1:6862462/1‑50 (MQ=255) aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc > 1:1407658/1‑49 (MQ=255) aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc > 1:7709569/1‑49 (MQ=255) aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc > 1:6904734/1‑49 (MQ=255) | CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG > NC_000913/257747‑257840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |