| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,440,194 | C→A | G279V (GGT→GTT) | rpoA ← | RNA polymerase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,440,194 | 0 | C | A | 83.5% | 300.3 / 75.2 | 127 | G279V (GGT→GTT) | rpoA | RNA polymerase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (12/9); new base A (53/53); total (65/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
AGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACA > NC_000913/3440146‑3440241 | aGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc < 1:6654747/50‑1 (MQ=255) gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGa > 1:168785/1‑50 (MQ=255) gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGa > 1:5666486/1‑50 (MQ=255) ttAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGa > 1:8125663/1‑49 (MQ=255) ttAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGa > 1:5308328/1‑49 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata < 1:107552/49‑1 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata < 1:4211885/49‑1 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata < 1:183170/49‑1 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata > 1:5180286/1‑49 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata < 1:2659789/49‑1 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGAtata < 1:406700/50‑1 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGAtata < 1:4484558/50‑1 (MQ=255) gCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAg > 1:2825614/1‑50 (MQ=255) cGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGt < 1:8133986/50‑1 (MQ=255) cGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGt > 1:3564662/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTg > 1:5906747/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTg > 1:1673752/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:6543073/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:8046154/1‑50 (MQ=255) ttttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTgg < 1:3015682/50‑1 (MQ=255) ttttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTgg < 1:30993/50‑1 (MQ=255) ttttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:3448018/1‑49 (MQ=255) ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa < 1:6748264/49‑1 (MQ=255) ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa < 1:7222711/49‑1 (MQ=255) ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa < 1:3208637/49‑1 (MQ=255) tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGa > 1:7538948/1‑48 (MQ=255) tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa > 1:3869692/1‑48 (MQ=255) tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa > 1:5436270/1‑48 (MQ=255) tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGAt < 1:3855468/49‑1 (MQ=255) aaGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa < 1:5846893/49‑1 (MQ=255) aaGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAg > 1:830696/1‑50 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAg < 1:8099758/49‑1 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGc > 1:6625238/1‑50 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGc > 1:268740/1‑50 (MQ=255) ggAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCt < 1:1988022/50‑1 (MQ=255) gAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt < 1:3472214/50‑1 (MQ=255) gAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCtt > 1:3613182/1‑50 (MQ=255) gAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCtt < 1:7003044/50‑1 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc < 1:3182134/50‑1 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc > 1:4848115/1‑50 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc < 1:827045/50‑1 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:4471594/50‑1 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:2830874/50‑1 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:1445485/50‑1 (MQ=255) cTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg > 1:8028435/1‑50 (MQ=255) cTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTg > 1:4186162/1‑50 (MQ=255) cTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTg < 1:4669585/50‑1 (MQ=255) tCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGc > 1:1435883/1‑50 (MQ=255) tCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGc > 1:7451901/1‑50 (MQ=255) tCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGc > 1:328220/1‑50 (MQ=255) tCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGc > 1:5321762/1‑50 (MQ=255) cAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCt > 1:8270831/1‑50 (MQ=255) cAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCt > 1:5710018/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCCTCTGCtt > 1:709615/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt < 1:1066373/50‑1 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt > 1:339160/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCt < 1:6937838/49‑1 (MQ=255) aCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt > 1:1907236/1‑49 (MQ=255) ccTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCttt > 1:2514031/1‑49 (MQ=255) ccTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTa < 1:5621899/50‑1 (MQ=255) cTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTaa < 1:3814003/50‑1 (MQ=255) cTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTaa < 1:5629008/50‑1 (MQ=255) cTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTaa < 1:4333911/50‑1 (MQ=255) cGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:1971900/1‑50 (MQ=255) ggTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAgg > 1:8084576/1‑49 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg < 1:391440/49‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAg < 1:4653407/48‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGc < 1:7169443/50‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGc < 1:2134623/50‑1 (MQ=255) gTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:4388470/1‑48 (MQ=255) gTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:405426/1‑48 (MQ=255) gTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:276822/1‑48 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg < 1:3810761/50‑1 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg > 1:3174794/1‑50 (MQ=255) aCGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt > 1:5985742/1‑50 (MQ=255) gCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt > 1:7357659/1‑48 (MQ=255) gCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt > 1:3562488/1‑48 (MQ=255) cTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg < 1:2654723/50‑1 (MQ=255) cTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:7471312/1‑50 (MQ=255) tGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:4098082/1‑49 (MQ=255) gTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:1119505/1‑48 (MQ=255) gTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:5133116/1‑48 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:8047154/1‑47 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGc < 1:1074324/48‑1 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGc < 1:6009029/48‑1 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGc < 1:4482485/48‑1 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCag < 1:4115881/50‑1 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCag < 1:5696712/50‑1 (MQ=255) aCCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCa > 1:4555318/1‑48 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag > 1:7283730/1‑50 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag < 1:5732188/50‑1 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga < 1:4349771/49‑1 (MQ=255) cAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc > 1:5245697/1‑50 (MQ=255) cAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc > 1:5317303/1‑50 (MQ=255) aGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg < 1:250674/50‑1 (MQ=255) gATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCgg < 1:7315384/50‑1 (MQ=255) gATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCgg < 1:2075631/50‑1 (MQ=255) aTCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa > 1:4784963/1‑50 (MQ=255) aTCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa > 1:2146924/1‑50 (MQ=255) aCCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACa < 1:2385514/49‑1 (MQ=255) | AGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACA > NC_000913/3440146‑3440241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |