| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,477,150 | C→T | K90K (AAG→AAA) | fkpA ← | peptidyl‑prolyl cis‑trans isomerase FkpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,477,150 | 0 | C | T | 99.3% | 542.0 / ‑3.8 | 139 | K90K (AAG→AAA) | fkpA | peptidyl‑prolyl cis‑trans isomerase FkpA |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (59/79); total (59/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01 | |||||||||||
Only 100 of 143 total aligned reads displayed.
CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA > NC_000913/3477104‑3477195 | cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt < 1:5297504/49‑1 (MQ=255)cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt < 1:1389118/49‑1 (MQ=255)cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt < 1:8136577/49‑1 (MQ=255) gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa < 1:5626690/49‑1 (MQ=255) gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa < 1:127334/49‑1 (MQ=255) gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt < 1:1724992/50‑1 (MQ=255) gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt < 1:7721373/50‑1 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt > 1:4458265/1‑49 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt > 1:3288577/1‑49 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt > 1:7066061/1‑49 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa < 1:6360234/50‑1 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa < 1:3513516/50‑1 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa < 1:5013358/50‑1 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa < 1:429014/50‑1 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa < 1:4206436/50‑1 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTAGGAGAGTTTGCTTTTATCa < 1:4532621/50‑1 (MQ=255) tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg < 1:4774563/50‑1 (MQ=255) tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg > 1:5096460/1‑50 (MQ=255) cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc > 1:353069/1‑49 (MQ=255) cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc > 1:6681774/1‑49 (MQ=255) cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc < 1:2299292/49‑1 (MQ=255) tttAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa > 1:6551357/3‑50 (MQ=255) tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa > 1:6780324/1‑50 (MQ=255) tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa > 1:2545707/1‑50 (MQ=255) gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa < 1:3160648/49‑1 (MQ=255) gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt > 1:2146259/1‑50 (MQ=255) tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg < 1:159756/50‑1 (MQ=255) agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg > 1:2471009/1‑49 (MQ=255) agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg > 1:3340430/1‑49 (MQ=255) gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa < 1:4666233/50‑1 (MQ=255) gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATCCa < 1:4666143/50‑1 (MQ=255) agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt < 1:4696430/50‑1 (MQ=255) tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc < 1:6606464/50‑1 (MQ=255) tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc < 1:7235368/50‑1 (MQ=255) tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc < 1:1413384/50‑1 (MQ=255) cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt > 1:6205002/1‑50 (MQ=255) tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt > 1:4045629/1‑49 (MQ=255) gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg > 1:2314495/1‑49 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt < 1:6476131/47‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg < 1:2849786/48‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg < 1:5222249/48‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa < 1:1909914/50‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa < 1:6613860/50‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa < 1:7939189/50‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa < 1:2077527/49‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa < 1:4870118/49‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa < 1:6847891/49‑1 (MQ=255) ttCGATCTCTTAGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt < 1:815043/47‑1 (MQ=39) tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa > 1:1229299/1‑49 (MQ=255) tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa > 1:326503/1‑49 (MQ=255) tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa > 1:477264/1‑49 (MQ=255) cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca > 1:5496444/1‑50 (MQ=255) cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca > 1:2626096/1‑50 (MQ=255) cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac < 1:5204754/49‑1 (MQ=255) gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:7151160/1‑50 (MQ=255) gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:7701078/1‑50 (MQ=255) gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:3147964/1‑50 (MQ=255) gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca < 1:208812/49‑1 (MQ=255) ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg > 1:3963862/1‑50 (MQ=255) ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc > 1:2357909/1‑49 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa < 1:7624941/46‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa < 1:4372818/46‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa < 1:2277934/46‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa < 1:2316891/46‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg < 1:638685/47‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc < 1:3461566/48‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc < 1:3573814/48‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa > 1:2361635/1‑50 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa > 1:4215043/1‑50 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt < 1:3613766/49‑1 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt > 1:1645717/1‑49 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt < 1:1125718/49‑1 (MQ=255) gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa < 1:3616443/50‑1 (MQ=255) gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa < 1:7734406/50‑1 (MQ=255) gTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATc < 1:6376910/49‑1 (MQ=255) tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg < 1:1724813/50‑1 (MQ=255) tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg < 1:2973041/50‑1 (MQ=255) cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc > 1:1105051/1‑50 (MQ=255) cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc > 1:1818815/1‑50 (MQ=255) cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc > 1:5053029/1‑50 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc < 1:6486604/49‑1 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc < 1:7906781/49‑1 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc < 1:7569286/49‑1 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc < 1:3106313/49‑1 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt > 1:8317108/1‑50 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt > 1:5892531/1‑50 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt < 1:3127817/50‑1 (MQ=255) gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt > 1:4388695/1‑49 (MQ=255) gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg < 1:2716533/50‑1 (MQ=255) agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa > 1:7383859/1‑50 (MQ=255) agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc > 1:955561/1‑50 (MQ=255) agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc < 1:6569313/50‑1 (MQ=255) agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc > 1:551571/1‑50 (MQ=255) gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt > 1:1027518/1‑50 (MQ=255) tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt < 1:3473227/50‑1 (MQ=255) tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt > 1:6591322/1‑50 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt < 1:6317898/49‑1 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa > 1:332917/1‑50 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa > 1:1635553/1‑50 (MQ=255) gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa < 1:3261188/49‑1 (MQ=255) | CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA > NC_000913/3477104‑3477195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |