BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	559.8 / NA	138	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (69/69); total (69/69)

Only 100 of 138 total aligned reads displayed.

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:4226405/1‑50 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:7657426/1‑49 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:1736131/1‑49 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             <  1:537757/49‑1 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:550468/1‑49 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                            <  1:1377062/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:6499316/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:3801484/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:430601/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:2430113/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:1189141/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           <  1:6172148/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:7413230/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:3293311/1‑49 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         <  1:3736308/50‑1 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:2973047/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  1:5736882/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        >  1:7225221/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  1:6053961/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        >  1:309103/1‑50 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       >  1:2253345/1‑50 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:4527007/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:7303440/50‑1 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:5221513/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:6067836/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:6784493/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:4201145/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:7590940/1‑50 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:6957362/1‑50 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    <  1:5163103/50‑1 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:2945701/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                   <  1:4399512/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:2798287/49‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:8026815/49‑1 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                <  1:3728883/50‑1 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                <  1:835409/50‑1 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGACAGTATGCCCGGGATATTCTACAg                                 >  1:687675/1‑49 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:7267810/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                >  1:809859/1‑49 (MQ=255)

               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:6170212/1‑49 (MQ=255)

               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:138008/1‑49 (MQ=255)

               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:2076372/1‑49 (MQ=255)

                tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                              <  1:8219235/49‑1 (MQ=255)

                tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                              <  1:1808143/49‑1 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             <  1:6661273/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           <  1:3567576/50‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           <  1:6948540/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:398764/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:7637575/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:5601917/1‑49 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:4485153/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:57433/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:686846/50‑1 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:6292574/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       <  1:6750873/49‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      <  1:5568587/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:2151783/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:5260995/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:4900451/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:2154546/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:4595280/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:4326270/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:4006460/1‑50 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                    <  1:6243997/50‑1 (MQ=255)

                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:1072746/1‑50 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                  <  1:4277505/50‑1 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:2254164/1‑50 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:3151282/1‑50 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:2319581/1‑49 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:1339859/1‑49 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               <  1:88820/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               <  1:480823/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               <  1:1880844/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  1:3481579/1‑50 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              >  1:2574190/1‑50 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              >  1:7662779/1‑50 (MQ=255)

                                aGACGATTGTATGCCCTGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:2898720/1‑50 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:8240421/1‑50 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:7702692/1‑49 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:364348/50‑1 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:7078720/50‑1 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          <  1:5772818/50‑1 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa           >  1:3881268/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  1:3652730/50‑1 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa        <  1:1076102/50‑1 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:2615172/1‑48 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:3431740/1‑48 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:1675396/1‑48 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:6605843/1‑50 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg      <  1:2880413/49‑1 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg      <  1:1856301/49‑1 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:3192709/1‑50 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:7935339/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:2654978/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:7987447/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:7221813/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:8123857/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:5558745/1‑50 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  1:8192986/49‑1 (MQ=255)

                                            gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  1:3832111/1‑49 (MQ=255)

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`