BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,720,597	Δ1 bp	coding (4/153 nt)	hokA ←	small toxic polypeptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,720,596	0	G	.	100.0%	635.5 / NA	127	coding (5/153 nt)	hokA	small toxic polypeptide
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (73/54); total (73/54)

Only 100 of 130 total aligned reads displayed.

TAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCA  >  NC_000913/3720547‑3720641

tAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCg                                               >  1:2123982/1‑50 (MQ=255)

 aaaaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCg                                               >  1:325154/1‑49 (MQ=255)

     gCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                                         <  1:5821037/50‑1 (MQ=255)

     gCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                                         <  1:374009/50‑1 (MQ=255)

     gCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                                         >  1:6449917/1‑50 (MQ=255)

     gCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                                         >  1:1786065/1‑50 (MQ=255)

      cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc                                        >  1:1082704/1‑50 (MQ=255)

      cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc                                        >  1:4705238/1‑50 (MQ=255)

      cGTGAAACAAATCACTATTAAAGAAAGAAATCTATATTTCTGC‑GCATTcc                                        >  1:33228/1‑50 (MQ=255)

       gTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTccc                                       <  1:67941/50‑1 (MQ=255)

        tGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc                                        >  1:7141120/1‑48 (MQ=255)

         gAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAg                                     >  1:2679118/1‑50 (MQ=255)

          aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAg                                     <  1:7255331/49‑1 (MQ=255)

          aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGc                                    <  1:7213067/50‑1 (MQ=255)

          aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGc                                    >  1:3271507/1‑50 (MQ=255)

          aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGc                                    >  1:2780367/1‑50 (MQ=255)

          aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGc                                    >  1:4202863/1‑50 (MQ=255)

          aaaCAAATCACTA‑TAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcc                                   >  1:3144888/1‑50 (MQ=255)

            aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcct                                  >  1:6848647/1‑50 (MQ=255)

            aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcct                                  >  1:3893253/1‑50 (MQ=255)

            aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcct                                  >  1:5231177/1‑50 (MQ=255)

            aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcct                                  <  1:5931676/50‑1 (MQ=255)

             cAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctc                                 >  1:4597446/1‑50 (MQ=255)

              aaaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcc                                <  1:6999301/50‑1 (MQ=255)

              aaaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcc                                <  1:1223748/50‑1 (MQ=255)

              aaaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcc                                >  1:5139497/1‑50 (MQ=255)

               aaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcct                               <  1:2623901/50‑1 (MQ=255)

                aTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtg                              >  1:6527445/1‑50 (MQ=255)

                 tCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt                             <  1:7194723/50‑1 (MQ=255)

                 tCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt                             >  1:803061/1‑50 (MQ=255)

                 tCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt                             <  1:1145458/50‑1 (MQ=255)

                 tCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt                             >  1:5964681/1‑50 (MQ=255)

                   aCTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgtg                            <  1:876463/49‑1 (MQ=255)

                   aCTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgtg                            <  1:415239/49‑1 (MQ=255)

                    cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt                          <  1:3767469/50‑1 (MQ=255)

                     tATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt                          >  1:7143525/1‑49 (MQ=255)

                      aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa                        >  1:4765905/1‑50 (MQ=255)

                       ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt                       <  1:2011181/50‑1 (MQ=255)

                       ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt                       >  1:5572182/1‑50 (MQ=255)

                       ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt                       >  1:6583500/1‑50 (MQ=255)

                        tAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt                      >  1:1446722/1‑50 (MQ=255)

                          aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt                     <  1:4840164/49‑1 (MQ=255)

                          aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt                     <  1:3948724/49‑1 (MQ=255)

                          aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc                    <  1:7385339/50‑1 (MQ=255)

                          aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc                    <  1:4461355/50‑1 (MQ=255)

                          aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc                    <  1:3743462/50‑1 (MQ=255)

                          aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc                    >  1:2356588/1‑50 (MQ=255)

                           agaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc                   <  1:4872926/50‑1 (MQ=255)

                           agaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc                   <  1:5625526/50‑1 (MQ=255)

                             aaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa                 >  1:4249125/1‑50 (MQ=255)

                             aaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa                 >  1:4922769/1‑50 (MQ=255)

                             aaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa                 >  1:6453285/1‑50 (MQ=255)

                              aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc                >  1:6411266/1‑50 (MQ=255)

                              aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc                <  1:4554329/50‑1 (MQ=255)

                              aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc                >  1:6172259/1‑50 (MQ=255)

                              aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc                <  1:2408853/50‑1 (MQ=255)

                               agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACg               <  1:875841/50‑1 (MQ=255)

                                gTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc                >  1:1051780/1‑48 (MQ=255)

                                 tAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg             <  1:1195658/50‑1 (MQ=255)

                                 tAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg             <  1:2939373/50‑1 (MQ=255)

                                  aaTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg             >  1:4566886/1‑49 (MQ=255)

                                  aaTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg             >  1:1218100/1‑49 (MQ=255)

                                  aaTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg             >  1:3548699/1‑49 (MQ=255)

                                  aaTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg             >  1:3693612/1‑49 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGt            <  1:2045573/49‑1 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGt            <  1:1829716/49‑1 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGt            <  1:3004958/49‑1 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTa           >  1:93850/1‑50 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTa           >  1:6588331/1‑50 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTa           >  1:5972826/1‑50 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTa           >  1:7595564/1‑50 (MQ=255)

                                   aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTa           >  1:5711318/1‑50 (MQ=255)

                                    tCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTa           >  1:42652/1‑49 (MQ=255)

                                      tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaa        >  1:3832600‑M1/1‑48 (MQ=255)

                                      tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaa        >  1:5622641‑M1/1‑48 (MQ=255)

                                      tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaa        >  1:4231785‑M1/1‑48 (MQ=255)

                                       atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac       >  1:1251908‑M1/1‑47 (MQ=255)

                                       atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac       >  1:1499742‑M1/1‑47 (MQ=255)

                                       atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac       >  1:4130813‑M1/1‑47 (MQ=255)

                                        tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTAt          <  1:827085/46‑1 (MQ=255)

                                        tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac       <  1:5278941‑M1/49‑4 (MQ=255)

                                        tatGTCTGC‑GCATTACCAGCCTCCTGTGTTGATTTCCAACGAGTATaac       <  1:3400613‑M1/49‑4 (MQ=255)

                                         atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactt     >  1:6115032‑M1/1‑45 (MQ=255)

                                         atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactt     >  1:6516028‑M1/1‑45 (MQ=255)

                                          tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    >  1:1397175‑M1/1‑44 (MQ=255)

                                          tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    <  1:3539562‑M1/50‑7 (MQ=255)

                                          tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    >  1:6649273‑M1/1‑44 (MQ=255)

                                          tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    >  1:3810709‑M1/1‑44 (MQ=255)

                                          tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    >  1:4882494‑M1/1‑44 (MQ=255)

                                          tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactt     <  1:1644267‑M1/49‑6 (MQ=255)

                                          tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaaatt     <  1:2590503‑M1/49‑6 (MQ=255)

                                           ttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    >  1:3929138‑M1/1‑43 (MQ=255)

                                           ttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    >  1:5060422‑M1/1‑43 (MQ=255)

                                            tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttga  >  1:2740500‑M1/1‑42 (MQ=255)

                                            tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttga  >  1:6333392‑M1/1‑42 (MQ=255)

                                            tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttga  >  1:4843467‑M1/1‑42 (MQ=255)

                                            tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttg   <  1:1588065‑M1/49‑8 (MQ=255)

                                            tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttg   <  1:5719875‑M1/49‑8 (MQ=255)

                                            tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttg   <  1:3952560‑M1/49‑8 (MQ=255)

                                            tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTACCAACGAGTATaactttg   <  1:5930307‑M1/49‑8 (MQ=255)

TAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCA  >  NC_000913/3720547‑3720641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`