Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	324.8 / NA	82	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/42);  total (40/42)

ATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118982‑119074                                               |                                               aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             >  1:3320161/1‑50 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             >  1:3276203/1‑50 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             >  1:2444083/1‑50 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             >  1:6785309/1‑50 (MQ=255)  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:1001151/50‑1 (MQ=255)  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:1396758/50‑1 (MQ=255)  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:4553854/50‑1 (MQ=255)  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:3570375/1‑50 (MQ=255)   aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:5534806/1‑49 (MQ=255)   aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:4100836/1‑49 (MQ=255)   aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:5403321/1‑49 (MQ=255)    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  1:6691432/49‑1 (MQ=255)    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  1:4002443/49‑1 (MQ=255)    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:3408287/1‑48 (MQ=255)     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         <  1:4915147/50‑1 (MQ=255)     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  1:1986004/1‑50 (MQ=255)     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  1:1452908/1‑50 (MQ=255)       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGt                                        <  1:3487943/49‑1 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:1619281/1‑50 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:3938414/1‑50 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      <  1:398905/50‑1 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      <  1:4457322/50‑1 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:6504823/1‑50 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      <  1:2429926/50‑1 (MQ=255)         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:7023245/1‑49 (MQ=255)         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                                     <  1:7535896/50‑1 (MQ=255)          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:4612304/1‑48 (MQ=255)          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCa                                    <  1:6263549/50‑1 (MQ=255)          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCa                                    <  1:4922246/50‑1 (MQ=255)            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  <  1:1683675/50‑1 (MQ=255)            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   <  1:731078/49‑1 (MQ=255)            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   <  1:6502414/49‑1 (MQ=255)             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:7012080/1‑50 (MQ=255)             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:6656976/1‑50 (MQ=255)              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                <  1:4519780/50‑1 (MQ=255)              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                >  1:4907790/1‑50 (MQ=255)               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  1:4108839/1‑50 (MQ=255)               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  1:2756871/1‑50 (MQ=255)                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               <  1:3471253/49‑1 (MQ=255)                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               <  1:2556487/49‑1 (MQ=255)                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  1:3873036/50‑1 (MQ=255)                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:5610656/1‑50 (MQ=255)                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  1:2949481/50‑1 (MQ=255)                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  1:4858297/50‑1 (MQ=255)                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  1:5918372/50‑1 (MQ=255)                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:5006741/1‑49 (MQ=255)                   ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            >  1:3464599/1‑49 (MQ=255)                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGa                           <  1:6262135/49‑1 (MQ=255)                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:5184990/1‑50 (MQ=255)                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:913199/49‑1 (MQ=255)                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        <  1:2292220/50‑1 (MQ=255)                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  1:47405/1‑50 (MQ=255)                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      <  1:5795376/50‑1 (MQ=255)                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      <  1:4826425/50‑1 (MQ=255)                         aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:6045888/1‑50 (MQ=255)                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:2257579/1‑49 (MQ=255)                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  1:2067256/50‑1 (MQ=255)                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  1:3778228/50‑1 (MQ=255)                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    >  1:6227449/1‑49 (MQ=255)                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    >  1:2825690/1‑49 (MQ=255)                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCa                   <  1:4638098/50‑1 (MQ=255)                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  1:2476883/1‑50 (MQ=255)                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:2821290/1‑50 (MQ=255)                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  1:5153479/50‑1 (MQ=255)                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:5002463/1‑50 (MQ=255)                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:5073225/1‑49 (MQ=255)                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  1:3182925/50‑1 (MQ=255)                               gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               >  1:2418775/1‑50 (MQ=255)                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:2797098/1‑50 (MQ=255)                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:304806/1‑50 (MQ=255)                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:3301426/1‑50 (MQ=255)                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:3413777/1‑50 (MQ=255)                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             <  1:5284895/50‑1 (MQ=255)                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  1:4810371/1‑50 (MQ=255)                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:6458127/1‑50 (MQ=255)                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          >  1:4089641/1‑50 (MQ=255)                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          <  1:4382166/50‑1 (MQ=255)                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt           <  1:4431136/49‑1 (MQ=255)                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         <  1:2032605/50‑1 (MQ=255)                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:5701870/1‑50 (MQ=255)                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         <  1:4372256/50‑1 (MQ=255)                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:6492833/1‑49 (MQ=255)                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:3628306/1‑49 (MQ=255)                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt        <  1:1185151/50‑1 (MQ=255)                                       tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa       <  1:5410480/50‑1 (MQ=255)                                        gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      >  1:4726192/1‑50 (MQ=255)                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  1:2636625/49‑1 (MQ=255)                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  1:3781380/49‑1 (MQ=255)                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  1:689068/49‑1 (MQ=255)                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  1:513619/1‑50 (MQ=255)                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    <  1:4978076/50‑1 (MQ=255)                                           ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   >  1:2080809/1‑50 (MQ=255)                                           ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   <  1:1767/50‑1 (MQ=255)                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:6971464/50‑1 (MQ=255)                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:7394064/50‑1 (MQ=255)                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   >  1:7468970/1‑49 (MQ=255)                                               |                                               ATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118982‑119074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑