Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	356.8 / NA	94	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/45);  total (49/45)

CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270764‑1270856                                                |                                              catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:2927234/1‑50 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                                            <  1:3855987/50‑1 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:6833775/1‑49 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             <  1:6677878/49‑1 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             <  1:6271743/49‑1 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:3710907/1‑49 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          <  1:6127606/49‑1 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          <  1:6284055/49‑1 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:6300748/1‑49 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                         <  1:7470990/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        <  1:2838614/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        >  1:6678738/1‑50 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        >  1:376076/1‑50 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        >  1:527271/1‑50 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        >  1:6168636/1‑50 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        <  1:5486542/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        <  1:602149/50‑1 (MQ=255)        tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      >  1:6375119/1‑50 (MQ=255)        tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      <  1:3407031/50‑1 (MQ=255)        tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      <  1:3352171/50‑1 (MQ=255)        tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      <  1:1722974/50‑1 (MQ=255)         aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                     >  1:389138/1‑50 (MQ=255)         aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                     >  1:3017892/1‑50 (MQ=255)         aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                     >  1:4092176/1‑50 (MQ=255)           gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                   <  1:1729358/50‑1 (MQ=255)           gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                   >  1:3191437/1‑50 (MQ=255)           gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                   <  1:4269503/50‑1 (MQ=255)            cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                  <  1:3028973/50‑1 (MQ=255)             ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 >  1:3964889/1‑50 (MQ=255)             ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 <  1:6445148/50‑1 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                >  1:5481995/1‑50 (MQ=255)               cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:845205/1‑50 (MQ=255)               cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               <  1:4890255/50‑1 (MQ=255)               cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:2756601/1‑50 (MQ=255)                aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                              <  1:1846286/50‑1 (MQ=255)                aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                              <  1:408497/50‑1 (MQ=255)                aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:2629382/1‑49 (MQ=255)                 aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:2844486/1‑48 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:2770871/1‑47 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:196179/1‑47 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            <  1:721913/50‑1 (MQ=255)                   cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                           <  1:5550369/50‑1 (MQ=255)                   cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            >  1:3183106/1‑49 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            >  1:3777674/1‑48 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            >  1:2051844/1‑48 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            >  1:1556602/1‑48 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            >  1:7567266/1‑48 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                          <  1:4482893/50‑1 (MQ=255)                     gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:890366/1‑50 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                        <  1:6703157/50‑1 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:4281809/1‑49 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:352316/1‑49 (MQ=255)                       gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:7378753/1‑48 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:5587816/1‑47 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:5846632/1‑47 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                      <  1:255593/50‑1 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                      <  1:7026570/50‑1 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                       <  1:1770419/49‑1 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                       <  1:364193/49‑1 (MQ=255)                         cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:319149/1‑46 (MQ=255)                           gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   <  1:4945798/50‑1 (MQ=255)                           gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:5105945/1‑50 (MQ=255)                             tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                 <  1:755117/50‑1 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                >  1:505205/1‑49 (MQ=255)                                gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:952671/1‑50 (MQ=255)                                gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:1851110/1‑50 (MQ=255)                                 ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac             <  1:6061913/50‑1 (MQ=255)                                 ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac             <  1:2446685/50‑1 (MQ=255)                                 ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac             <  1:6222714/50‑1 (MQ=255)                                 ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac             <  1:3009616/50‑1 (MQ=255)                                 ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:5359954/1‑49 (MQ=255)                                  tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:1133731/1‑48 (MQ=255)                                  tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:257102/1‑48 (MQ=255)                                  tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:7541176/1‑48 (MQ=255)                                   gcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg           >  1:2511341/1‑50 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca            <  1:4989532/48‑1 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca            <  1:1343001/48‑1 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg           >  1:1981694/1‑49 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg           >  1:7497858/1‑49 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg           <  1:2764568/49‑1 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc          <  1:4786606/50‑1 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc          <  1:5099002/50‑1 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa         <  1:2918051/50‑1 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa         >  1:4517986/1‑50 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:134148/1‑49 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:5876308/1‑49 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       <  1:3057080/50‑1 (MQ=255)                                        aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       >  1:757004/1‑49 (MQ=255)                                         ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa     <  1:2980503/50‑1 (MQ=255)                                         ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa     <  1:3427281/50‑1 (MQ=255)                                         ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa     <  1:773802/50‑1 (MQ=255)                                           gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:5947851/1‑47 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:7587900/50‑1 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag   <  1:5426140/49‑1 (MQ=255)                                                |                                              CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270764‑1270856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑