Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	392.4 / NA	96	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/65);  total (31/65)

CGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382511‑1382601                                             |                                               cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCa                                            <  1:6918129/49‑1 (MQ=255)  gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:6884683/1‑49 (MQ=255)  gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:2154110/50‑1 (MQ=255)   aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          >  1:4144812/1‑49 (MQ=255)   aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          >  1:7228112/1‑49 (MQ=255)   aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:6870903/50‑1 (MQ=255)   aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGAAGACAGTCGCAATa                                         <  1:4209576/50‑1 (MQ=38)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:6137309/1‑50 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:1332224/1‑50 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:3936851/1‑50 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:4995810/1‑50 (MQ=255)     tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:7173985/50‑1 (MQ=255)     tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:5364857/50‑1 (MQ=255)       aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                     <  1:3514783/50‑1 (MQ=255)       aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:4346272/49‑1 (MQ=255)       aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:2079997/49‑1 (MQ=255)        aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    <  1:1467977/50‑1 (MQ=255)        aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:6850419/1‑50 (MQ=255)        aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:4756655/1‑50 (MQ=255)        aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    <  1:3688611/50‑1 (MQ=255)        aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    <  1:2590786/50‑1 (MQ=255)        aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    <  1:381832/50‑1 (MQ=255)        aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:1476418/1‑50 (MQ=255)        aCTATTATCTCGAGCGCGGCATCAATATGGACGACAGTCGCAATAACggg                                    >  1:360738/1‑49 (MQ=255)          tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:5176571/1‑48 (MQ=255)           attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 >  1:2392394/1‑50 (MQ=255)            ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGa                                  <  1:3753266/48‑1 (MQ=255)            ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 <  1:1517458/49‑1 (MQ=255)            ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                <  1:5255045/50‑1 (MQ=255)             tatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               >  1:3052273/1‑50 (MQ=255)              atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               <  1:260407/49‑1 (MQ=255)               tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              <  1:4382547/49‑1 (MQ=255)                  cGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          >  1:4536946/1‑50 (MQ=255)                   gAGTGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          <  1:3120499/49‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                             <  1:4656404/46‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:2843951/48‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:234487/48‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:1416987/47‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          <  1:5487314/49‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:4359470/1‑50 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:1372756/50‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:6877787/50‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:7445028/50‑1 (MQ=255)                    aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:5866932/50‑1 (MQ=255)                    aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        >  1:2272104/1‑50 (MQ=255)                     gcgcGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       <  1:5170968/50‑1 (MQ=255)                      cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:5319951/1‑49 (MQ=255)                      cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCa                      <  1:3265444/50‑1 (MQ=255)                       gcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCaa                     <  1:1277467/50‑1 (MQ=255)                        cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:6336925/1‑47 (MQ=255)                        cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:1833994/1‑47 (MQ=255)                        cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:3154816/50‑1 (MQ=255)                         ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    >  1:2675460/1‑49 (MQ=255)                         ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:3905795/50‑1 (MQ=255)                         ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:78933/50‑1 (MQ=255)                         ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:2023866/50‑1 (MQ=255)                          gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaa                  <  1:39057/50‑1 (MQ=255)                           cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:6360704/50‑1 (MQ=255)                           cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:5708716/50‑1 (MQ=255)                           cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:2155180/50‑1 (MQ=255)                           cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 >  1:7236428/1‑50 (MQ=255)                           cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:3109739/50‑1 (MQ=255)                            ttCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                >  1:4502324/1‑50 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:4504099/49‑1 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:5045634/49‑1 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:7210606/48‑1 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              >  1:2246923/1‑50 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              >  1:5569176/1‑50 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             <  1:4952210/50‑1 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:1908889/1‑50 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:3484490/49‑1 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:3337626/49‑1 (MQ=255)                                atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            >  1:4021388/1‑50 (MQ=255)                                atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            >  1:219084/1‑50 (MQ=255)                                atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            <  1:7352044/50‑1 (MQ=255)                                 tatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgcg           <  1:2096471/50‑1 (MQ=255)                                  atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:4463166/1‑50 (MQ=255)                                   tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          <  1:3324137/49‑1 (MQ=255)                                   tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          <  1:2969532/49‑1 (MQ=255)                                   tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         <  1:5628741/50‑1 (MQ=255)                                     gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt        <  1:920003/49‑1 (MQ=255)                                     gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt        <  1:3056268/49‑1 (MQ=255)                                     gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       <  1:2048207/50‑1 (MQ=255)                                      acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:6744962/50‑1 (MQ=255)                                      acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:6115840/50‑1 (MQ=255)                                      acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:4220016/50‑1 (MQ=255)                                      acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:4114934/50‑1 (MQ=255)                                       cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:6796439/50‑1 (MQ=255)                                       cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:4163170/50‑1 (MQ=255)                                       cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     >  1:1941922/1‑50 (MQ=255)                                        gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    >  1:51105/1‑50 (MQ=255)                                        gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:1598631/50‑1 (MQ=255)                                         acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:3387382/50‑1 (MQ=255)                                          cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:4095232/50‑1 (MQ=255)                                          cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:3893287/50‑1 (MQ=255)                                          cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:1069190/50‑1 (MQ=255)                                             |                                               CGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382511‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑