Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	380.4 / NA	92	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (49/43);  total (49/43)

TGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742709‑1742800                                               |                                              tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                            >  1:2900467/1‑50 (MQ=255)   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          <  1:2827593/50‑1 (MQ=255)   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           <  1:3388889/49‑1 (MQ=255)   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:5722210/1‑49 (MQ=255)   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:7119831/1‑49 (MQ=255)    gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:5003277/1‑49 (MQ=255)    gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:5439933/1‑49 (MQ=255)    gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:7603931/1‑49 (MQ=255)       aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      >  1:7505460/1‑50 (MQ=255)         gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     >  1:492242/1‑49 (MQ=255)          ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   >  1:7092973/1‑50 (MQ=255)           tttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  >  1:6837207/1‑50 (MQ=255)           tttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  >  1:1199945/1‑50 (MQ=255)            ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:3177039/49‑1 (MQ=255)            ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:6669019/49‑1 (MQ=255)             tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 <  1:5490077/49‑1 (MQ=255)             tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:4876045/1‑49 (MQ=255)             tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:1133179/1‑49 (MQ=255)             tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:6315349/1‑49 (MQ=255)             tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 <  1:6292525/49‑1 (MQ=255)              ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                <  1:3643168/49‑1 (MQ=255)               tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                               <  1:1563766/49‑1 (MQ=255)                cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                              <  1:5312248/49‑1 (MQ=255)                cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                              <  1:3489866/49‑1 (MQ=255)                cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                              <  1:1531337/49‑1 (MQ=255)                 ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             >  1:3145442/1‑49 (MQ=255)                 ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             >  1:2548937/1‑49 (MQ=255)                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           <  1:5527168/49‑1 (MQ=255)                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           <  1:6670368/49‑1 (MQ=255)                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           <  1:1104116/49‑1 (MQ=255)                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                            <  1:357886/48‑1 (MQ=255)                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             <  1:3820862/47‑1 (MQ=255)                    ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         <  1:2462593/50‑1 (MQ=255)                    ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          <  1:6989579/49‑1 (MQ=255)                    ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          <  1:7105079/49‑1 (MQ=255)                     cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:4241391/50‑1 (MQ=255)                      aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       >  1:5853598/1‑50 (MQ=255)                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:4115372/1‑50 (MQ=255)                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      <  1:47490/50‑1 (MQ=255)                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:7515368/1‑50 (MQ=255)                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:6811602/1‑50 (MQ=255)                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      <  1:2454984/50‑1 (MQ=255)                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:2509727/1‑50 (MQ=255)                        gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:7129826/50‑1 (MQ=255)                        gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:2596288/50‑1 (MQ=255)                        gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:5614786/50‑1 (MQ=255)                         agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:2408985/50‑1 (MQ=255)                         agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    >  1:623471/1‑50 (MQ=255)                         agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:2931578/50‑1 (MQ=255)                          gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   >  1:2658198/1‑50 (MQ=255)                           tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  >  1:940752/1‑50 (MQ=255)                           tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  >  1:2129844/1‑50 (MQ=255)                           tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  >  1:3163529/1‑50 (MQ=255)                           tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:3233670/50‑1 (MQ=255)                           tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:4067702/50‑1 (MQ=255)                            cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:3733298/1‑50 (MQ=255)                            cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:5504347/1‑50 (MQ=255)                            cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:1697827/1‑50 (MQ=255)                             gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:4997334/1‑50 (MQ=255)                             gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:2902784/1‑50 (MQ=255)                              tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:4274330/1‑49 (MQ=255)                              tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg               <  1:3072923/50‑1 (MQ=255)                              tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg               <  1:2084272/50‑1 (MQ=255)                               gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTgc              >  1:1661712/1‑49 (MQ=255)                               gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              >  1:3415814/1‑50 (MQ=255)                               gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              >  1:767611/1‑50 (MQ=255)                                cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              <  1:5559286/49‑1 (MQ=255)                                cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             >  1:5824395/1‑50 (MQ=255)                                 gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            <  1:3233103/50‑1 (MQ=255)                                  ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:882487/1‑50 (MQ=255)                                  ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:3991897/1‑50 (MQ=255)                                   tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          >  1:5530427/1‑50 (MQ=255)                                    ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:2796857/49‑1 (MQ=255)                                    ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:4473504/49‑1 (MQ=255)                                    ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:5577098/49‑1 (MQ=255)                                    ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         >  1:5661107/1‑50 (MQ=255)                                     cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc        >  1:2686463/1‑50 (MQ=255)                                     cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc        >  1:3735271/1‑50 (MQ=255)                                      aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:6637239/50‑1 (MQ=255)                                      aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:4125721/50‑1 (MQ=255)                                       gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:3465282/1‑49 (MQ=255)                                       gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg      <  1:3532735/50‑1 (MQ=255)                                        tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:1050697/1‑50 (MQ=255)                                        tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:4228062/1‑50 (MQ=255)                                        tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:4336480/1‑50 (MQ=255)                                         gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    >  1:2425739/1‑50 (MQ=255)                                         gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    >  1:6035630/1‑50 (MQ=255)                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    <  1:2408024/49‑1 (MQ=255)                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    <  1:2186765/49‑1 (MQ=255)                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg   >  1:6078015/1‑50 (MQ=255)                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg   <  1:3164870/50‑1 (MQ=255)                                            tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  <  1:1872462/49‑1 (MQ=255)                                               |                                              TGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742709‑1742800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑