Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 257,794 T→C intergenic (+23/‑35) frsA → / → crl fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913257,7940TC100.0% 550.9 / NA 131intergenic (+23/‑35)frsA/crlfermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (71/60);  total (71/60)

Only 100 of 131 total aligned reads displayed.

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840
                                               |                                              
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:2582257/50‑1 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:943876/50‑1 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:5022922/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:1354639/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:1366196/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:6892512/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:6277500/1‑50 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             <  1:6086408/48‑1 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           <  1:4316845/50‑1 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:5739593/1‑49 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:7509555/1‑49 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:6625720/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  1:7472320/50‑1 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  1:2192650/50‑1 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          >  1:6449312/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          >  1:4649402/1‑49 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:5519183/1‑50 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:3312408/1‑49 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:2035812/1‑49 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:152314/50‑1 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:7429264/1‑48 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:3821756/1‑48 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa                                      <  1:2461500/50‑1 (MQ=255)
         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     >  1:5991207/1‑50 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    <  1:7565272/50‑1 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  1:6369661/1‑50 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  1:7362245/1‑50 (MQ=255)
           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   >  1:2444182/1‑50 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  1:1243001/50‑1 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGTTAAAACAGTTg                                  >  1:5705068/1‑49 (MQ=39)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                >  1:4820836/1‑50 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                <  1:6047307/50‑1 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                <  1:5064682/50‑1 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                >  1:1168416/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:2990888/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               <  1:4577945/50‑1 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:6796603/1‑50 (MQ=255)
                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:2779884/1‑49 (MQ=255)
                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:5321245/1‑49 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:7216567/1‑50 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:6813788/1‑50 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             <  1:1939036/49‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             <  1:2734501/49‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                              <  1:5561149/48‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                           <  1:6868820/50‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            <  1:655290/49‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            >  1:947113/1‑49 (MQ=255)
                    gtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:144668/1‑50 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                         <  1:3952510/50‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                         <  1:694613/50‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          <  1:10550/49‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:6219016/1‑49 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:7292289/1‑49 (MQ=255)
                      tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:1386762/1‑48 (MQ=255)
                      tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:848975/1‑48 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:6782584/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:2877666/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:7180939/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:5495317/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:2401997/1‑50 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACACTTGCATCACAACAg                       >  1:4805530/1‑50 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAgg                      <  1:2055806/50‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:5138034/1‑49 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:6580515/1‑49 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:7165008/1‑49 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:2753936/1‑48 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGga                     <  1:3447638/50‑1 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGga                     <  1:5172266/50‑1 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:5757457/1‑47 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:2954123/1‑46 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   <  1:1162129/50‑1 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:5948205/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt                  <  1:6600298/50‑1 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                 <  1:3270809/49‑1 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:4308736/1‑50 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  1:2564232/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  1:1631031/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  1:3774663/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:366909/49‑1 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCa              <  1:6374933/50‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:1837237/49‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            >  1:6658398/1‑49 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:514777/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          <  1:1688176/50‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:1777784/1‑50 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         >  1:3852294/1‑50 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         >  1:6950884/1‑50 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         <  1:4740043/50‑1 (MQ=255)
                                      tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        >  1:1581447/1‑50 (MQ=255)
                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:442785/49‑1 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       <  1:3005528/49‑1 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:6348495/1‑49 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  1:4165361/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     >  1:267427/1‑50 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    >  1:4802211/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:3129450/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2136316/1‑50 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2434826/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:6395357/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:5060112/50‑1 (MQ=255)
                                               |                                              
CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑