Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,843,412	C→T	R12H (CGT→CAT)	hycH ←	formate hydrogenlyase assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,843,412	0	C	T	100.0%	309.9 / NA	81	R12H (CGT→CAT)	hycH	formate hydrogenlyase assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/42);  total (39/42)

CCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGCC  >  NC_000913/2843365‑2843458                                                |                                               ccTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATgg                                              >  1:4255469/1‑50 (MQ=255)  ctgctgCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGc                                             >  1:5891091/1‑50 (MQ=255)  ctgctgCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGc                                             >  1:6469182/1‑50 (MQ=255)  ctgctgCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGc                                             >  1:6893190/1‑50 (MQ=255)    gctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                            >  1:5292932/1‑49 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                            <  1:4116786/48‑1 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                            >  1:3513962/1‑48 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                            <  1:4439688/48‑1 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                            <  1:3696215/48‑1 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                            <  1:3705839/48‑1 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCa                                          <  1:6675106/50‑1 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCa                                          <  1:2576924/50‑1 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCa                                          <  1:4712971/50‑1 (MQ=255)      tgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                                         >  1:2575855/1‑50 (MQ=255)      tgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                                         >  1:319196/1‑50 (MQ=255)       gcgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGt                                        >  1:6459628/1‑50 (MQ=255)        cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                                         <  1:1319519/48‑1 (MQ=255)        cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                                         <  1:7508517/48‑1 (MQ=255)        cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGtt                                       <  1:5249594/50‑1 (MQ=255)        cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGt                                        <  1:1785028/49‑1 (MQ=255)         gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGtt                                       >  1:4134253/1‑49 (MQ=255)         gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTg                                      <  1:7048407/50‑1 (MQ=255)          ccTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGa                                     <  1:5948962/50‑1 (MQ=255)           cTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGAc                                    <  1:5797265/50‑1 (MQ=255)              ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                                   <  1:3148012/48‑1 (MQ=255)              ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                                   <  1:2530077/48‑1 (MQ=255)              ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGa                                 <  1:5454407/50‑1 (MQ=255)                 gggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAaca                              >  1:4690455/1‑50 (MQ=255)                  ggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac                             <  1:857063/50‑1 (MQ=255)                  ggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac                             <  1:4954414/50‑1 (MQ=255)                    cgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcacca                           <  1:3012047/50‑1 (MQ=255)                     gTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcaccac                          >  1:6645205/1‑50 (MQ=255)                     gTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcaccac                          >  1:1172678/1‑50 (MQ=255)                     gTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcaccac                          >  1:5621461/1‑50 (MQ=255)                     gTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACAGAAcaccac                          >  1:2635665/1‑50 (MQ=255)                        gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt                       >  1:3709900/1‑50 (MQ=255)                        gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt                       <  1:4612470/50‑1 (MQ=255)                        gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt                       <  1:810928/50‑1 (MQ=255)                         cATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCttt                      <  1:7364242/50‑1 (MQ=255)                         cATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCttt                      >  1:7187211/1‑50 (MQ=255)                         cATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCttt                      >  1:2810227/1‑50 (MQ=255)                          aTCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt                     <  1:6838417/50‑1 (MQ=255)                          aTCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt                     >  1:6705529/1‑50 (MQ=255)                           tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt                     >  1:4084372/1‑49 (MQ=255)                           tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt                     >  1:2038401/1‑49 (MQ=255)                           tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTc                    <  1:1368612/50‑1 (MQ=255)                           tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTc                    <  1:6843090/50‑1 (MQ=255)                            cGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                   >  1:5734367/1‑50 (MQ=255)                            cGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                   >  1:3256220/1‑50 (MQ=255)                            cGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                   >  1:3495720/1‑50 (MQ=255)                             gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                   <  1:5231135/49‑1 (MQ=255)                             gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                   <  1:2423207/49‑1 (MQ=255)                             gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc                  >  1:6705784/1‑50 (MQ=255)                              ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                 >  1:6469585/1‑50 (MQ=255)                              ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                 >  1:2127344/1‑50 (MQ=255)                              ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                 <  1:2346326/50‑1 (MQ=255)                              ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                 <  1:6128260/50‑1 (MQ=255)                               tctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                 >  1:3643701/1‑49 (MQ=255)                               tctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                 >  1:5767742/1‑49 (MQ=255)                                ctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa               >  1:4526566/1‑50 (MQ=255)                                ctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa               >  1:4440405/1‑50 (MQ=255)                                   atcaATAAATTTATGGCTCAGTTGACTGAACACCGCCTTTTCACTCATc             <  1:7504479/49‑1 (MQ=39)                                   atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa               <  1:3319128/47‑1 (MQ=255)                                   atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCAt              <  1:6536258/48‑1 (MQ=255)                                   atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg            >  1:7073924/1‑50 (MQ=255)                                   atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg            <  1:5642972/50‑1 (MQ=255)                                    tcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCgg           <  1:5010768/50‑1 (MQ=255)                                    tcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg            >  1:5083371/1‑49 (MQ=255)                                     caATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa          >  1:4431789/1‑50 (MQ=255)                                      aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa          >  1:7495615/1‑49 (MQ=255)                                      aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa          >  1:3625190/1‑49 (MQ=255)                                      aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGAt         <  1:261201/50‑1 (MQ=255)                                       aTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATa        <  1:2392009/50‑1 (MQ=255)                                        tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc       <  1:921671/50‑1 (MQ=255)                                        tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc       <  1:2391509/50‑1 (MQ=255)                                        tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc       <  1:4228691/50‑1 (MQ=255)                                          aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgc     <  1:5907737/50‑1 (MQ=255)                                          aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcg      <  1:6797037/49‑1 (MQ=255)                                            tttATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACgcgc   >  1:7510651/1‑50 (MQ=255)                                             ttATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGcc  >  1:7598890/1‑50 (MQ=255)                                             ttATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGcc  >  1:5304931/1‑50 (MQ=255)                                                |                                               CCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGCC  >  NC_000913/2843365‑2843458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑