BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	499.0 / NA	127	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (52/75); total (52/75)

Only 100 of 128 total aligned reads displayed.

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:928519/49‑1 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  1:2418899/1‑50 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:605250/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:5836872/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:2213607/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  1:1023278/50‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:91048/1‑50 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:7115684/1‑50 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  1:6034751/50‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:4554354/1‑50 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:3772071/1‑50 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:4278501/1‑49 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:2070505/1‑49 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                           <  1:3875433/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:3709710/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:6690176/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:3257936/50‑1 (MQ=255)

    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:7054703/1‑50 (MQ=255)

    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:4194964/1‑50 (MQ=255)

     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:2005100/1‑49 (MQ=255)

     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:4193449/1‑49 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:5876246/49‑1 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        >  1:471681/1‑49 (MQ=255)

       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:674032/1‑49 (MQ=255)

        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:572033/1‑50 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:1530925/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:5755813/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:5287934/48‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:171147/48‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:154652/49‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:2398239/50‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:4670189/50‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  1:4747581/49‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  1:3729452/49‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                 >  1:6253261/1‑50 (MQ=255)

             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:800039/50‑1 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:2582648/1‑50 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:1197724/1‑50 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:4134995/1‑50 (MQ=255)

                cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:1054911/1‑50 (MQ=255)

                cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:4092217/1‑50 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:698048/1‑49 (MQ=255)

                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            >  1:1607116/1‑49 (MQ=255)

                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            >  1:4354276/1‑49 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  1:2340256/47‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:6177593/48‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:3834692/48‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:769422/1‑50 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                           <  1:826052/49‑1 (MQ=255)

                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                         <  1:802972/49‑1 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:4158991/1‑50 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:6869362/1‑50 (MQ=255)

                       aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:1932354/1‑49 (MQ=255)

                       aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:1981329/1‑49 (MQ=255)

                         ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    >  1:2550379/1‑50 (MQ=255)

                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:3692520/1‑49 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  1:6692674/44‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  1:6805346/44‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:2066763/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:5741564/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:2160548/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:357933/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:5202644/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:2749748/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:6433763/48‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 <  1:821475/48‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:2218823/49‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:796898/49‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:6130119/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:870235/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:6182288/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:5821567/50‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:1300240/50‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:6955463/50‑1 (MQ=255)

                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  1:3385313/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:6290412/49‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:2587021/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:1359124/1‑50 (MQ=255)

                                   gggagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:4132429/48‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:4402030/1‑49 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:5479527/50‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:4173162/50‑1 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  1:843650/50‑1 (MQ=255)

                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        <  1:2113690/50‑1 (MQ=255)

                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        >  1:213274/1‑50 (MQ=255)

                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       <  1:6069945/50‑1 (MQ=255)

                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:1110271/1‑50 (MQ=255)

                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       <  1:5213255/50‑1 (MQ=255)

                                      agTTTGCTTTTATCAGCAAATGCATCCAGAACACCAGCGATCAGCTGATc       <  1:635161/50‑1 (MQ=38)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:1206769/50‑1 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:1062313/1‑50 (MQ=255)

                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     <  1:982626/50‑1 (MQ=255)

                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     >  1:7077497/1‑50 (MQ=255)

                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     >  1:1018580/1‑50 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:1110836/49‑1 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:6052119/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   <  1:4721134/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   <  1:3228557/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   <  1:2361940/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:113042/1‑50 (MQ=255)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`