Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,438,124 T→C W140R (TGG→CGG)  ytfI → protein YtfI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,438,1240TC100.0% 525.6 / NA 128W140R (TGG→CGG) ytfIprotein YtfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (64/64);  total (64/64)

Only 100 of 128 total aligned reads displayed.

CAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438078‑4438169
                                              |                                             
cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:3809983/1‑49 (MQ=255)
 aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                            <  1:2120017/49‑1 (MQ=255)
 aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                            <  1:2359647/49‑1 (MQ=255)
 aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:5596296/1‑50 (MQ=255)
 aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           <  1:4740827/50‑1 (MQ=255)
 aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:2717962/1‑50 (MQ=255)
 aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:2371258/1‑50 (MQ=255)
  aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:2173901/50‑1 (MQ=255)
  aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:5818749/1‑49 (MQ=255)
  aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:6423574/1‑49 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:3866546/1‑50 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:5978465/1‑50 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:5983865/1‑50 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:6292964/1‑50 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:4683179/1‑50 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         <  1:6465066/50‑1 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:2114385/1‑50 (MQ=255)
   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:1618796/1‑50 (MQ=255)
       cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:4670579/50‑1 (MQ=255)
       cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:6223653/50‑1 (MQ=255)
        gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    <  1:3745369/50‑1 (MQ=255)
         tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:4376561/1‑49 (MQ=255)
         tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:6690162/1‑49 (MQ=255)
         tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                   <  1:7473351/50‑1 (MQ=255)
         tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                   <  1:5382607/50‑1 (MQ=255)
         tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                   <  1:3460669/50‑1 (MQ=255)
           aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:6977734/1‑47 (MQ=255)
           aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:2191120/49‑1 (MQ=255)
           aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:5073629/49‑1 (MQ=255)
           aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:3147115/49‑1 (MQ=255)
           aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:6706893/50‑1 (MQ=255)
            aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 >  1:5660271/1‑49 (MQ=255)
            aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                <  1:115381/50‑1 (MQ=255)
            aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                <  1:3093044/50‑1 (MQ=255)
             tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:1062675/50‑1 (MQ=255)
             tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:5170850/50‑1 (MQ=255)
              ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:4781111/1‑49 (MQ=255)
                ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  1:3113383/1‑49 (MQ=255)
                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:3049652/1‑50 (MQ=255)
                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:795570/1‑50 (MQ=255)
                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           <  1:6338338/50‑1 (MQ=255)
                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  1:524734/49‑1 (MQ=255)
                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  1:6213574/49‑1 (MQ=255)
                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  1:6825901/49‑1 (MQ=255)
                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  1:2764249/49‑1 (MQ=255)
                  gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:5569390/1‑49 (MQ=255)
                   ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:848731/1‑48 (MQ=255)
                   ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:2791762/1‑48 (MQ=255)
                   ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:6056797/1‑48 (MQ=255)
                     aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       >  1:4407266/1‑50 (MQ=255)
                      gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       <  1:421111/49‑1 (MQ=255)
                      gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      <  1:237268/50‑1 (MQ=255)
                      gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      <  1:5447036/50‑1 (MQ=255)
                      gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      <  1:7395765/50‑1 (MQ=255)
                      gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      >  1:7114733/1‑50 (MQ=255)
                       aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:4751978/1‑50 (MQ=255)
                       aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:4833967/1‑50 (MQ=255)
                       aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:4054917/50‑1 (MQ=255)
                       aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:959191/50‑1 (MQ=255)
                        aCAAAACAGACGATTGTATGCCCGAGATATTCTACAGAAAAAACAGTCTg                    <  1:3771071/50‑1 (MQ=255)
                         cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:4168133/1‑50 (MQ=255)
                         cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:6783784/1‑50 (MQ=255)
                          aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:4429145/1‑49 (MQ=255)
                          aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:617033/1‑49 (MQ=255)
                          aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:2902049/1‑49 (MQ=255)
                           aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 <  1:1365131/50‑1 (MQ=255)
                            aaCAGACGATTGTATGCCCGGGATATTCTACATAAAAAACAGTCTGACa                 >  1:5614050/1‑49 (MQ=255)
                            aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:1165770/1‑49 (MQ=255)
                             aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  1:6757034/1‑50 (MQ=255)
                             aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  1:2934821/1‑50 (MQ=255)
                              cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              <  1:3980365/50‑1 (MQ=255)
                               aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:4729713/50‑1 (MQ=255)
                               aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:1302379/1‑50 (MQ=255)
                               aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:1491259/1‑50 (MQ=255)
                                gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:4443221/1‑49 (MQ=255)
                                gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:11110/50‑1 (MQ=255)
                                gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:1241525/50‑1 (MQ=255)
                                gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:621415/50‑1 (MQ=255)
                                gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:7411591/50‑1 (MQ=255)
                                 aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            >  1:5913319/1‑49 (MQ=255)
                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  1:4246886/50‑1 (MQ=255)
                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:3548384/1‑49 (MQ=255)
                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:755157/1‑49 (MQ=255)
                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:1789808/1‑49 (MQ=255)
                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:3358988/1‑49 (MQ=255)
                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:3780927/1‑49 (MQ=255)
                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:6642727/1‑49 (MQ=255)
                                   gATTGTATGCACGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  1:4875477/50‑1 (MQ=255)
                                    aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa        <  1:5392134/50‑1 (MQ=255)
                                    aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:4806726/1‑48 (MQ=255)
                                       gTATGCCCGGGGTATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:4821831/1‑50 (MQ=255)
                                       gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg      <  1:3880783/49‑1 (MQ=255)
                                       gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg      <  1:4682923/49‑1 (MQ=255)
                                       gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:5564201/1‑50 (MQ=255)
                                        tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:6769586/49‑1 (MQ=255)
                                        tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:2663014/49‑1 (MQ=255)
                                        tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:7138381/49‑1 (MQ=255)
                                         aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:161389/1‑49 (MQ=255)
                                          tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  1:5381501/49‑1 (MQ=255)
                                           gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  1:3470108/1‑49 (MQ=255)
                                              |                                             
CAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438078‑4438169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑