Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,776,2500GT24.6% 199.9 / 14.9 65V222L (GTG→TTG) ydiFputative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (17/32);  new base T (16/0);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 2.73e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAA  >  NC_000913/1776205‑1776300
                                             |                                                  
gACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATtgtg                                                  <  1:5488207/48‑1 (MQ=255)
gACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATtgt                                                   <  1:1378976/47‑1 (MQ=255)
gACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGtgat                                                >  1:844484/1‑50 (MQ=255)
 aCGCATTGGTTATTGTCCAGGCGGTGCACAATAACGGCGGCTTTTtgat                                                >  1:5942258/1‑49 (MQ=255)
 aCGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGtgatg                                               <  1:480873/50‑1 (MQ=255)
   gCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTgttgat                                             >  1:2127346/1‑50 (MQ=255)
   gCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTggtgat                                             >  1:747067/1‑50 (MQ=255)
   gCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTgatgat                                             <  1:2117943/50‑1 (MQ=255)
    cATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGgtgatg                                            >  1:5050063/1‑50 (MQ=255)
    cATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGatgatg                                            >  1:5585956/1‑50 (MQ=255)
    cATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGatgatg                                            <  1:552195/50‑1 (MQ=255)
    cATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGatgatg                                            <  1:2255007/50‑1 (MQ=255)
      ttGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTggtgatt                                            >  1:3016434/1‑47 (MQ=38)
      ttGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGatgatg                                            >  1:6178499/1‑48 (MQ=255)
        ggTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGATTATTCAgg                                        >  1:4277454/1‑50 (MQ=255)
         gTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGt                                       <  1:5330064/50‑1 (MQ=255)
          ttATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGGAgggg                                      >  1:422503/1‑48 (MQ=255)
          ttATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTg                                      <  1:5174469/50‑1 (MQ=255)
          ttATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTg                                      <  1:5475718/50‑1 (MQ=255)
           tATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGGTGATGGAgggg                                      >  1:4849166/1‑47 (MQ=37)
            aTTGCCCAGGCGGTGCACAATAACGGCGGTCTTTTGATGTTTCAgggg                                      >  1:3016814/1‑46 (MQ=255)
            aTTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGc                                     <  1:1130126/49‑1 (MQ=255)
              tGCCCAGGCGGTGCACAATAACGGCGGTATTTTGATGATGCAGGTGCAg                                   >  1:3822106/1‑49 (MQ=255)
                cccAGGCGGTGCACAATAACGGCGGTATTTTGATGATGCAGGGGGAGaa                                 >  1:249521/1‑49 (MQ=38)
                cccAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGaaa                                <  1:692546/50‑1 (MQ=255)
                cccAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGaa                                 <  1:5841864/49‑1 (MQ=255)
                 ccAGGCGGTGCATAATAACGGCGGTATTTTGATGATGCCGGGGGAGaaa                                >  1:5943985/1‑49 (MQ=25)
                 ccAGGCGGTGCACAATAACGGCGGTATTTTGATGATTGAGGGGCAGaaaa                               >  1:5798659/1‑50 (MQ=255)
                  cAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAAt                              <  1:449595/50‑1 (MQ=255)
                   aGGCGGTGCACAATAACGGCGGTATTTTTTTGATGCAGGGGCAGaaaaag                             >  1:6252464/1‑48 (MQ=255)
                   aGGCGGTGCACAATAACGGCGGTATTTTTGTGTTGGAAGGGCCGAAAATg                             >  1:3508716/1‑50 (MQ=255)
                   aGGCGGTGCACAATAACGGCGGTATTTTGATGATTCCGGTGCAGaaaaag                             >  1:1450661/1‑48 (MQ=255)
                   aGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATg                             <  1:91581/50‑1 (MQ=255)
                    ggCGGTGCACAATAACGGCGGTATTGTGGTGGTGCAGGTGCAGAAAAAgg                            >  1:4287299/1‑50 (MQ=38)
                    ggCGGTGCACAATAACGGCGGTATTGTGATGATGCCGGGGCAGAAAAAgg                            >  1:2731755/1‑50 (MQ=255)
                     gCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGt                           <  1:1078524/50‑1 (MQ=255)
                     gCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGt                           <  1:3685049/50‑1 (MQ=255)
                     gCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGt                           <  1:3107220/50‑1 (MQ=255)
                      cGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGGGCAGAAAAAGGt                           >  1:5773473/1‑49 (MQ=38)
                      cGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGtt                          <  1:1404831/50‑1 (MQ=255)
                      cGGTGCACAATAACGGCGGTATTGTGATGATGCAGGGGCAGAAAATggg                           >  1:6326522/1‑48 (MQ=255)
                      cGGTGCACAATAACGGCGGCATTTTTCTGATGGCGGGGCAGAAAATGGt                           >  1:894057/1‑49 (MQ=16)
                       ggTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTa                         <  1:543337/50‑1 (MQ=255)
                        gTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTaa                        <  1:5875413/50‑1 (MQ=255)
                        gTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTaa                        <  1:283113/50‑1 (MQ=255)
                           cacaATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGaa                     <  1:2720506/50‑1 (MQ=255)
                           cacaATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGaa                     <  1:5656899/50‑1 (MQ=255)
                           cacaATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGa                      <  1:1578862/49‑1 (MQ=255)
                              aaTAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGc                  <  1:4244785/50‑1 (MQ=255)
                                tAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCa                <  1:4060780/50‑1 (MQ=255)
                                  aCGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCAc               <  1:2695796/49‑1 (MQ=255)
                                  aCGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACg              <  1:4050753/50‑1 (MQ=255)
                                  aCGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACg              <  1:1956495/50‑1 (MQ=255)
                                    ggcggTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCt            >  1:3378054/1‑50 (MQ=255)
                                     gcggTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTg           <  1:3914196/50‑1 (MQ=255)
                                     gcggTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTg           >  1:1482195/1‑50 (MQ=255)
                                      cggTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTg           >  1:875524/1‑49 (MQ=255)
                                      cggTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGc          <  1:5667124/50‑1 (MQ=255)
                                       ggTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCa         >  1:270143/1‑50 (MQ=255)
                                       ggTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCa         >  1:5100985/1‑50 (MQ=255)
                                         tATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATc       >  1:2471815/1‑50 (MQ=255)
                                         tATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATc       <  1:3953807/50‑1 (MQ=255)
                                         tATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATc       <  1:3563892/50‑1 (MQ=255)
                                          aTTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATcc      >  1:3316126/1‑50 (MQ=255)
                                           ttGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATcc      >  1:2269628/1‑49 (MQ=255)
                                           ttGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATcc      >  1:221793/1‑49 (MQ=255)
                                           ttGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCt     <  1:4706912/50‑1 (MQ=255)
                                            tgtgATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCt     >  1:3764243/1‑49 (MQ=255)
                                            tgtgATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCt     >  1:3800647/1‑49 (MQ=255)
                                            tgtgATGATGCAGGTG‑‑GAAAATGGTTAAGAAAGCCACGCTGCATCCTaaa  >  1:3188687/1‑50 (MQ=255)
                                             gtgATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTa    >  1:2392016/1‑49 (MQ=255)
                                             gtgATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTa    <  1:2539516/49‑1 (MQ=255)
                                             gtgATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTa    >  1:5413637/1‑49 (MQ=255)
                                             |                                                  
GACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAA  >  NC_000913/1776205‑1776300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: