| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,423,287 | 0 | G | C | 38.1% | 31.6 / 11.1 | 22 | intergenic (+93/+136) | yhdZ/rrfF | putative ABC transporter ATP‑binding subunit YhdZ/5S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (1/12); new base C (8/0); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCT > NC_000913/3423238‑3423307 | gCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCg < 1:5996647/50‑1 (MQ=255) cTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCCCCCGcc > 1:4351718/1‑50 (MQ=18) cTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCCCCCTGcccccc > 1:267418/1‑50 (MQ=18) tttCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGccccccc > 1:1896230/1‑49 (MQ=255) cGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAcc < 1:4014031/49‑1 (MQ=255) gCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAccc < 1:3591453/49‑1 (MQ=255) gCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGccccccccccc > 1:34649/1‑49 (MQ=14) cTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCCCCAccccc > 1:1987223/1‑48 (MQ=14) cTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCAccccc > 1:472242/1‑48 (MQ=14) cTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGcccccccccccc > 1:2625248/1‑48 (MQ=14) tgatttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCccccccccccccc > 1:3170538/1‑49 (MQ=14) tgatttgatGTCTGGCAGTTTATGGCGGGCGTCCCGCccccccccccccc > 1:40366/1‑49 (MQ=14) tgatttgatGTCTGGCAGTTTATGGCGGGCGGACTCCTCACCAACCTCCg > 1:711097/1‑50 (MQ=9) gatttgatGTCTGGCAGTTTATGGCGGTCGTCCTTTCCCCCCCCCTcccg > 1:6380796/1‑48 (MQ=14) gatttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCcccccccccccccg > 1:594059/1‑48 (MQ=14) gatttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTcc < 1:1117806/48‑1 (MQ=40) gatttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCg < 1:1035816/49‑1 (MQ=40) atttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCgg < 1:5930233/49‑1 (MQ=38) tttgatGTCTGGCAGTTTATGGCGGGGGTCCTGGCCCCCACCCTCCgggg > 1:2763527/1‑49 (MQ=11) ttgatGTCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCTCCCGGcc > 1:2518820/1‑50 (MQ=11) tgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGcc < 1:3555216/49‑1 (MQ=38) tgatGTCTGGCAGTTTATGGCGGGCGCGCTCCCCCCCCCCCCCCCGGCCg > 1:2011804/1‑50 (MQ=2) atGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGtt < 1:3433136/50‑1 (MQ=38) atGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGtt < 1:2191612/50‑1 (MQ=38) atGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGt < 1:6192735/49‑1 (MQ=38) atGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCggt > 1:5488077/1‑48 (MQ=11) gTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGc > 1:3783871/1‑50 (MQ=11) tCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGc < 1:6360358/49‑1 (MQ=32) tCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCt < 1:3264248/50‑1 (MQ=32) tCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCt < 1:3197681/50‑1 (MQ=32) | GCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCT > NC_000913/3423238‑3423307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |