| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,610,466 | 0 | T | G | 32.8% | 134.7 / 13.6 | 64 | G351G (GGT→GGG) | prfC | peptide chain release factor RF3 |
| Reads supporting (aligned to +/- strand): ref base T (7/36); new base G (21/0); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGC > NC_000913/4610417‑4610515 | gcACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGt < 1:2048843/50‑1 (MQ=255) aCTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGa < 1:1088179/50‑1 (MQ=255) cTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGAc > 1:5339977/1‑50 (MQ=255) gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGGCCg > 1:2544928/1‑50 (MQ=38) gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGACCg > 1:2596599/1‑50 (MQ=255) cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGAcc < 1:387096/48‑1 (MQ=255) cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGGCCgg > 1:5965566/1‑49 (MQ=38) cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGACCgc > 1:519630/1‑49 (MQ=255) gAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGAGCggt > 1:3574843/1‑48 (MQ=37) gAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGAGCGtt > 1:2637395/1‑50 (MQ=38) aaaGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTc < 1:4771693/50‑1 (MQ=255) aaaGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTc < 1:3664216/50‑1 (MQ=255) aaaGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGGCCCTTc > 1:6120167/1‑50 (MQ=37) aaaGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGAGCGTTc > 1:6182531/1‑50 (MQ=38) aaGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGAACGTTCg > 1:3014805/1‑50 (MQ=38) aGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGACCGTTCGc > 1:2196360/1‑50 (MQ=255) aGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGGGACAGTTcat > 1:983003/1‑48 (MQ=38) gATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCa < 1:4316164/50‑1 (MQ=255) aTGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCAc < 1:2035059/50‑1 (MQ=255) aTGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCAc < 1:129829/50‑1 (MQ=255) tggtgATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGtt < 1:1123408/50‑1 (MQ=255) tggtgATCTCCGACGCGCTGACCTTCATGGCGGGGGACCGGTCCCCCGtt > 1:2030430/1‑50 (MQ=255) ggtgATCTCCGACGCGCTGACCTTTATGGCGGGGGAACGGTCGCACGGTg > 1:4911027/1‑50 (MQ=255) gtgATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTga < 1:814280/50‑1 (MQ=255) tgATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTgaa < 1:4832884/50‑1 (MQ=255) tgATCTCCGACGCGCTGACCTTTATGGCGGGGGACCGTTTGCACGTTg > 1:2868164/1‑48 (MQ=38) tctcCGACGCGCTGACCTTTATGGCGGGGGACCGGTCCCACGGTgtaga > 1:3760214/1‑49 (MQ=255) ctcCGACGCGCTGACCTTTATGGCGGGGGACCGTTCGCCCGGTtaag > 1:2362146/1‑47 (MQ=255) ccGACGCGCTGACCTTTATGGCGGGTGGCCGTTCGCCCGGTGaagaagag > 1:3935156/1‑48 (MQ=255) cGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTgaagaa < 1:3685243/46‑1 (MQ=255) cGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTgaaga < 1:973600/45‑1 (MQ=255) cGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGc < 1:2153884/48‑1 (MQ=255) cGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCg < 1:1591119/49‑1 (MQ=255) cGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCg < 1:6276402/49‑1 (MQ=255) cGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCg < 1:307754/49‑1 (MQ=255) cGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGt < 1:6020577/50‑1 (MQ=255) cGACGCGCTGACCTTTATGGCGGGGGGCCGGTCGCACCTTGaagaaggg > 1:14130/1‑47 (MQ=255) cGACGCGCTGACCTTTATGGCGGGGGAGCGTTCGCCCGTTGAAGAAACg > 1:3189682/1‑49 (MQ=255) gACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTa < 1:5049537/50‑1 (MQ=255) gACGCGCTGACCTTTATGGCGGGGTAGCGCTCGCCCGTTGAAGAAACGTa > 1:5718776/1‑50 (MQ=255) aCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTAt < 1:852941/50‑1 (MQ=255) aCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTAt < 1:2101674/50‑1 (MQ=255) aCGCGCTGACCTTTATGGCGGGGGACCGGTCCCACGTTTGAGAAGCGGAt > 1:2021650/1‑50 (MQ=255) cgcgCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATc < 1:4360454/50‑1 (MQ=255) cgcgCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATc < 1:5718216/50‑1 (MQ=255) gcgcTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATcc < 1:4990053/50‑1 (MQ=255) cgcTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCg < 1:557262/50‑1 (MQ=255) ccTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGat < 1:3562121/50‑1 (MQ=255) tATGGCTGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATcc > 1:4447576/1‑50 (MQ=255) tATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGatat < 1:1716077/48‑1 (MQ=255) tATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGatat < 1:563928/48‑1 (MQ=255) tATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATc < 1:5600929/49‑1 (MQ=255) tATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATc < 1:4291261/49‑1 (MQ=255) tGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCt < 1:6689699/49‑1 (MQ=255) tGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTc < 1:4222254/50‑1 (MQ=255) ggCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCg > 1:871051/1‑50 (MQ=255) ggCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCg > 1:4988741/1‑50 (MQ=255) gCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCgg < 1:159363/50‑1 (MQ=255) gCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCgg > 1:3977771/1‑50 (MQ=255) gCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCgg < 1:3660972/50‑1 (MQ=255) gCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCg < 1:5854056/49‑1 (MQ=255) gCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCg < 1:6324368/49‑1 (MQ=255) gggTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGcc > 1:2613947/1‑50 (MQ=255) ggTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCt > 1:4138224/1‑50 (MQ=255) ggTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCt < 1:1163601/50‑1 (MQ=255) tGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGc < 1:2902935/50‑1 (MQ=255) | GCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGC > NC_000913/4610417‑4610515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |