| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 87,417 | G→T | G21C (GGC→TGC) | ilvH → | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 87,417 | 0 | G | T | 98.9% | 321.5 / ‑6.5 | 89 | G21C (GGC→TGC) | ilvH | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (49/39); minor base C (1/0); total (50/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGC > NC_000913/87370‑87463 | tatCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGc > 1:2950239/1‑50 (MQ=255) atCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGcc > 1:6069280/1‑50 (MQ=255) atCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGcc > 1:1027970/1‑50 (MQ=255) atCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGcc > 1:90219/1‑50 (MQ=255) atCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGcc > 1:3775391/1‑50 (MQ=255) tCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGctt > 1:1854099/1‑48 (MQ=255) tCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGcc < 1:1503509/49‑1 (MQ=255) tCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCt > 1:2185707/1‑50 (MQ=255) cAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtt > 1:4279587/1‑50 (MQ=255) cAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtt > 1:4625129/1‑50 (MQ=255) aGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtt < 1:1851319/49‑1 (MQ=255) gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtttt < 1:2424492/50‑1 (MQ=255) gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtttt < 1:5653720/50‑1 (MQ=255) gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtttt > 1:2025047/1‑50 (MQ=255) tCTTACTAGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCttttt < 1:4080653/50‑1 (MQ=38) cTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtttttt > 1:5743663/1‑50 (MQ=255) cTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtttttt > 1:5992227/1‑50 (MQ=255) ttACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTc > 1:2032668/1‑50 (MQ=255) ttACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTc > 1:5246765/1‑50 (MQ=255) ttACTCGAAAATGAATCAGGCGCGTAATCCCGCGAGATTCGCCTTTTTTc > 1:3729790/1‑50 (MQ=255) tACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTcc > 1:2790441/1‑50 (MQ=255) aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtttttt < 1:2822062/47‑1 (MQ=255) aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCtttttt < 1:2917654/47‑1 (MQ=255) aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTc < 1:5082384/48‑1 (MQ=255) aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTc < 1:3763109/48‑1 (MQ=255) cTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTccc < 1:2228059/49‑1 (MQ=255) cTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTcc > 1:2566128/1‑48 (MQ=255) cTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCa < 1:1978398/50‑1 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAg < 1:3027112/48‑1 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGc < 1:2764734/49‑1 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGc < 1:5775891/49‑1 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGc < 1:4914632/49‑1 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCg > 1:6087039/1‑50 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCg > 1:885854/1‑50 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCg > 1:6573361/1‑50 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGCGATTTGCCTTTTTTCCCAGCg > 1:6707692/1‑50 (MQ=255) aaaTGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTg > 1:279376/1‑50 (MQ=255) aaaTGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTg > 1:1116408/1‑50 (MQ=255) aaTGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTgg > 1:2450040/1‑50 (MQ=255) aTGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGc > 1:6566069/1‑50 (MQ=255) tGAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCt < 1:6293483/50‑1 (MQ=255) gAATCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTa > 1:2116183/1‑50 (MQ=255) aaTCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTac < 1:4230064/50‑1 (MQ=255) aaTCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTa < 1:5377794/49‑1 (MQ=255) aaTCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTa > 1:929055/1‑49 (MQ=255) aaTCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTa < 1:2158732/49‑1 (MQ=255) aaTCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTa > 1:3055177/1‑49 (MQ=255) tCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTacaa < 1:3967095/50‑1 (MQ=255) tCAGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTacaa > 1:588782/1‑50 (MQ=255) aGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTacaaca > 1:5896022/1‑50 (MQ=255) aGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTacaac < 1:1287231/49‑1 (MQ=255) aGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTacaac < 1:612302/49‑1 (MQ=255) aGGCGCGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTacaac < 1:2268718/49‑1 (MQ=255) gcgcGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACAtt < 1:3680532/50‑1 (MQ=255) gcgcGTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACAtt > 1:5883924/1‑50 (MQ=255) cgcgTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTg < 1:2015016/50‑1 (MQ=255) gcgTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTg > 1:6455298/1‑49 (MQ=255) cgTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGaa < 1:1404263/50‑1 (MQ=255) cgTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGaa < 1:2208626/50‑1 (MQ=255) gTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGa > 1:2802590/1‑48 (MQ=255) gTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGa > 1:5988389/1‑48 (MQ=255) gTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGa > 1:6571096/1‑48 (MQ=255) gTTATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGa > 1:1426021/1‑48 (MQ=255) ttATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAg < 1:1278371/50‑1 (MQ=255) tATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGc > 1:143318/1‑50 (MQ=255) tATCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGc > 1:3370038/1‑50 (MQ=255) aTCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGcc > 1:4896084/1‑50 (MQ=255) tCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGcc < 1:2845905/49‑1 (MQ=255) tCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCt < 1:191182/50‑1 (MQ=255) tCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCt < 1:6135371/50‑1 (MQ=255) tCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCt < 1:6743315/50‑1 (MQ=255) tCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCt < 1:341521/50‑1 (MQ=255) tCCCGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCt > 1:2140387/1‑50 (MQ=255) cccGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCt > 1:3602043/1‑49 (MQ=255) ccGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGa < 1:2450437/50‑1 (MQ=255) ccGCGTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGa > 1:314278/1‑50 (MQ=255) gcgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGAcc > 1:5752332/1‑50 (MQ=255) gcgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGAcc < 1:4439167/50‑1 (MQ=255) gcgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGAcc < 1:3288127/50‑1 (MQ=255) gcgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGAcc > 1:3797442/1‑50 (MQ=255) cgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCg > 1:2171537/1‑50 (MQ=255) cgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCg < 1:4794249/50‑1 (MQ=255) cgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCg > 1:6745156/1‑50 (MQ=255) cgTGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCg > 1:1165474/1‑50 (MQ=255) tGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGtt > 1:2765838/1‑50 (MQ=255) tGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGtt > 1:3182370/1‑50 (MQ=255) tGATTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGtt > 1:716829/1‑50 (MQ=255) aTTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTgc < 1:6041092/50‑1 (MQ=255) aTTTGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTg < 1:564490/49‑1 (MQ=255) | TATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGC > NC_000913/87370‑87463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |