Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	308.6 / NA	84	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/42);  total (42/42)

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270765‑1270856                                               |                                              atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                                            <  1:4676805/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:5037896/1‑49 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             <  1:2795612/49‑1 (MQ=255)  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                           <  1:4220828/50‑1 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:3341206/1‑50 (MQ=255)    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          <  1:2640860/49‑1 (MQ=255)    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                         <  1:6076711/50‑1 (MQ=255)    aaaTAGGCGGCAAACAGGGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                         <  1:6213661/50‑1 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        >  1:604038/1‑50 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                       <  1:6698783/50‑1 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                       <  1:2514311/50‑1 (MQ=255)       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      <  1:5018652/50‑1 (MQ=255)        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                     >  1:3396356/1‑50 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                     <  1:3733347/49‑1 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                    >  1:4831121/1‑50 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                    >  1:6472942/1‑50 (MQ=255)           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                  <  1:4756268/50‑1 (MQ=255)           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                  >  1:1240504/1‑50 (MQ=255)           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGCCCg                                  >  1:6519366/1‑50 (MQ=39)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 >  1:5430657/1‑50 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 <  1:2185970/50‑1 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 <  1:2156019/50‑1 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 <  1:3119983/50‑1 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 <  1:5037482/50‑1 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 <  1:2588312/50‑1 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 >  1:1585347/1‑50 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               <  1:4126467/50‑1 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:5421352/1‑50 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                              <  1:6128149/50‑1 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                              <  1:5587090/50‑1 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:2706307/1‑49 (MQ=255)                aaCAGTGCCAGATGCGCTGCGCCATTGACCATGTTAGTACGTCCGCTg                               >  1:2135299/1‑48 (MQ=37)                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:3249370/1‑47 (MQ=255)                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            <  1:1591311/50‑1 (MQ=255)                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            >  1:6488398/1‑48 (MQ=255)                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                          <  1:1264224/50‑1 (MQ=255)                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                        <  1:3413691/50‑1 (MQ=255)                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:6100202/1‑48 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:3947253/1‑47 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:2003595/1‑47 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                      <  1:558894/50‑1 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                      <  1:6593456/50‑1 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:3685753/1‑46 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:5554226/1‑46 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:2622693/1‑46 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:6577423/1‑46 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:6474892/1‑45 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:3667260/1‑50 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   <  1:3481746/50‑1 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   <  1:3806501/50‑1 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:4615381/1‑50 (MQ=255)                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  <  1:1204336/50‑1 (MQ=255)                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  <  1:741482/50‑1 (MQ=255)                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  <  1:1427814/50‑1 (MQ=255)                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  >  1:312717/1‑50 (MQ=255)                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                 >  1:348105/1‑50 (MQ=255)                             gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                >  1:338566/1‑50 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                >  1:4008484/1‑49 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                <  1:1942196/49‑1 (MQ=255)                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:6706774/1‑50 (MQ=255)                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              <  1:2780770/50‑1 (MQ=255)                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:6730115/1‑48 (MQ=255)                                 tgcccCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:2725595/1‑48 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca            <  1:102394/48‑1 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:845935/1‑50 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        <  1:3174519/50‑1 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:4499987/1‑50 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:3820610/1‑50 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       <  1:5699102/50‑1 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       <  1:6608669/50‑1 (MQ=255)                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       >  1:1853184/1‑49 (MQ=255)                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       >  1:5832375/1‑49 (MQ=255)                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       >  1:2551708/1‑49 (MQ=255)                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      <  1:3680504/50‑1 (MQ=255)                                        ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa     <  1:4012166/50‑1 (MQ=255)                                        ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa     <  1:4905227/50‑1 (MQ=255)                                        ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:6742734/1‑49 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:2955994/1‑47 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:3413979/1‑47 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:4514665/1‑47 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:378125/50‑1 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:1804674/50‑1 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:3659393/50‑1 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag   <  1:1855151/49‑1 (MQ=255)                                               |                                              ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270765‑1270856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑