Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	328.9 / NA	81	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (43/38);  total (43/38)

GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742708‑1742800                                                |                                              gTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGa                                             <  1:1655718/50‑1 (MQ=255)   gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:3182575/1‑50 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:4928427/1‑49 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:1472528/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                         <  1:206902/50‑1 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                       >  1:1139137/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                       >  1:5249977/1‑50 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      >  1:3029442/1‑50 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:6012275/50‑1 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:1767831/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     <  1:2316586/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     <  1:5187781/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     <  1:4616581/50‑1 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                    <  1:1210841/50‑1 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                    <  1:3331733/50‑1 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   <  1:4038466/50‑1 (MQ=255)            tttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  >  1:3198911/1‑50 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:4118673/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:1691796/49‑1 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 <  1:3264424/49‑1 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 <  1:201785/49‑1 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 <  1:4150716/49‑1 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                               <  1:4291698/49‑1 (MQ=255)                 cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                              <  1:1010278/49‑1 (MQ=255)                 cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                              <  1:5478374/49‑1 (MQ=255)                   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           >  1:5441981/1‑50 (MQ=255)                   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           >  1:202584/1‑50 (MQ=255)                   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           >  1:6026765/1‑50 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           <  1:4792170/49‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         >  1:355493/1‑50 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         >  1:6746223/1‑50 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          <  1:6355150/49‑1 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       >  1:2986382/1‑50 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       <  1:43552/50‑1 (MQ=255)                        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:3685549/1‑50 (MQ=255)                        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:2410301/1‑50 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:1905206/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     >  1:1341442/1‑50 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     >  1:799824/1‑50 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     >  1:1204830/1‑50 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:2744096/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:2136298/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:423719/50‑1 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   <  1:3042902/50‑1 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   <  1:560499/50‑1 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   >  1:4378719/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   >  1:231585/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   >  1:3497195/1‑50 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  >  1:6398827/1‑50 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:1365630/50‑1 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:5107031/50‑1 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:4256872/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:6438680/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                <  1:3180413/50‑1 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:2640613/1‑50 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:2162903/1‑49 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:706494/1‑49 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:1357972/1‑49 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              <  1:820734/49‑1 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              <  1:2021136/49‑1 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:4587984/1‑50 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:5995071/1‑50 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:3524434/1‑50 (MQ=255)                                    tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          >  1:4947928/1‑50 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:1884921/49‑1 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:3387202/49‑1 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         >  1:5903140/1‑50 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         >  1:1506420/1‑50 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc        <  1:3719387/50‑1 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:4083100/1‑50 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:6570009/50‑1 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:1186161/50‑1 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:339809/50‑1 (MQ=255)                                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:4717602/1‑49 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAACAACGCGTTGGCGTGACTTCGCCGa     >  1:6596114/1‑50 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGTGTTGGCGTGACTTCGCCGa     >  1:2634082/1‑50 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:5849393/1‑50 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:3963067/1‑50 (MQ=255)                                          gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    >  1:6383406/1‑50 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg   >  1:6020994/1‑50 (MQ=255)                                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  >  1:2019341/1‑50 (MQ=255)                                                |                                              GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742708‑1742800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑