Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 257,794 T→C intergenic (+23/‑35) frsA → / → crl fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913257,7940TC100.0% 433.6 / NA 104intergenic (+23/‑35)frsA/crlfermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (58/46);  total (58/46)

Only 100 of 104 total aligned reads displayed.

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840
                                               |                                              
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              >  1:2413842/1‑50 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:1246984/50‑1 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              >  1:2773833/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:2320936/1‑50 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                            <  1:2528326/49‑1 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:5622368/1‑49 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:5508257/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  1:3711474/50‑1 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:2034620/1‑50 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:4876711/1‑49 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:3660196/50‑1 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:3845096/1‑48 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:3946309/1‑48 (MQ=255)
         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     <  1:4248357/50‑1 (MQ=255)
         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     <  1:859914/50‑1 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  1:1891231/1‑50 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  1:1439895/1‑50 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  1:6584717/1‑50 (MQ=255)
           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   <  1:386398/50‑1 (MQ=255)
           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   >  1:1493985/1‑50 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  1:184920/50‑1 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  1:3454755/50‑1 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGc                                 <  1:3792237/50‑1 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGc                                 <  1:3275070/50‑1 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                >  1:513721/1‑50 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                >  1:4408374/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:5397954/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:5758050/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:5845794/1‑50 (MQ=255)
                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:78712/1‑49 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:1248322/1‑50 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:6651754/1‑50 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:6487002/1‑50 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             <  1:4163576/49‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:6344434/1‑49 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                              <  1:326113/48‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                              <  1:3808813/48‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            >  1:4128829/1‑49 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            <  1:4042706/49‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            >  1:1904994/1‑49 (MQ=255)
                    gtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:4951119/1‑50 (MQ=255)
                    gtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:3663989/1‑50 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                         <  1:3374765/50‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                         <  1:1255017/50‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:6243514/1‑49 (MQ=255)
                      tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:1134546/1‑48 (MQ=255)
                      tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:6697205/1‑48 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:2050571/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:4367100/1‑50 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAgg                      <  1:2131739/50‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:2067198/49‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:1804192/49‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:6681849/1‑49 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:6726961/49‑1 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:1548594/1‑48 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGga                     <  1:3704562/50‑1 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGga                     <  1:2924774/50‑1 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:246639/1‑47 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                    <  1:1858206/50‑1 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:41270/1‑46 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:3120221/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:340366/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt                  <  1:4827397/50‑1 (MQ=255)
                             ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:2501420/1‑48 (MQ=255)
                               gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                <  1:5436354/49‑1 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  1:4224221/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  1:1028141/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCa              <  1:714526/50‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:1343648/49‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:366350/49‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:5228949/49‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:2339257/49‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg           <  1:4701722/50‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:1201500/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:5915053/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:3418492/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:305871/1‑50 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         <  1:428159/50‑1 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         >  1:1967912/1‑50 (MQ=255)
                                      tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        >  1:5574712/1‑50 (MQ=255)
                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:2353761/49‑1 (MQ=255)
                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:1544438/49‑1 (MQ=255)
                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:4908579/49‑1 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:590218/1‑49 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:3933982/1‑49 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:836104/1‑49 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       <  1:72081/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  1:1233950/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     >  1:495577/1‑50 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     >  1:5370041/1‑50 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     >  1:3515808/1‑50 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     >  1:6692020/1‑50 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    >  1:3450385/1‑50 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    >  1:2054349/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   <  1:494514/50‑1 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   <  1:5648840/50‑1 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2698709/1‑50 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2099459/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:1672718/50‑1 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:4470402/50‑1 (MQ=255)
                                               |                                              
CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑