Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	217.0 / NA	55	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/24);  total (31/24)

CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270764‑1270856                                                |                                              catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:4915807/1‑50 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                                            <  1:4867356/50‑1 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:1050255/1‑49 (MQ=255)    caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:4109975/1‑50 (MQ=255)    caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:2173954/1‑50 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          <  1:2764962/49‑1 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:2135668/1‑49 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:1999006/1‑49 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:2039815/1‑49 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                         <  1:2523046/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        <  1:1154464/50‑1 (MQ=255)       aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                       >  1:2054029/1‑50 (MQ=255)       aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                       <  1:2802864/50‑1 (MQ=255)       aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                       <  1:821161/50‑1 (MQ=255)        tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      >  1:2679362/1‑50 (MQ=255)        tAGGCGGCAAACAGTGCCAGATGCGCTGCACCATTGAGCATGTTAGTACg                                      >  1:3802569/1‑50 (MQ=39)          ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                    <  1:4781317/50‑1 (MQ=255)            cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                  >  1:2220132/1‑50 (MQ=255)            cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                  <  1:5035670/50‑1 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                <  1:4352984/50‑1 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                >  1:3182987/1‑50 (MQ=255)               cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               <  1:584662/50‑1 (MQ=255)                aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:738050/1‑49 (MQ=255)                aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:3790644/1‑49 (MQ=255)                 aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                             <  1:1869139/50‑1 (MQ=255)                 aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                             <  1:4462138/50‑1 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            <  1:4542014/50‑1 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            >  1:2635070/1‑48 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                          <  1:3816352/50‑1 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                        <  1:4515165/50‑1 (MQ=255)                       gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:1275266/1‑48 (MQ=255)                         cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:4350613/1‑46 (MQ=255)                          aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                     >  1:3334084/1‑49 (MQ=255)                          aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                    <  1:3560426/50‑1 (MQ=255)                           gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:2940801/1‑50 (MQ=255)                           gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:1192754/1‑50 (MQ=255)                            aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  >  1:3288679/1‑50 (MQ=255)                            aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  <  1:1283484/50‑1 (MQ=255)                            aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  >  1:1600493/1‑50 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                >  1:2645079/1‑49 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                >  1:2605444/1‑49 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                >  1:4700655/1‑49 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                <  1:509444/49‑1 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc               <  1:1668607/50‑1 (MQ=255)                                gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:859251/1‑50 (MQ=255)                                 ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:2136440/1‑49 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa         >  1:686838/1‑50 (MQ=255)                                      ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        <  1:4794721/50‑1 (MQ=255)                                      ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:2585749/1‑50 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       <  1:2435963/50‑1 (MQ=255)                                        aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       >  1:1652198/1‑49 (MQ=255)                                         ttGAGCATGTTAGTACGTCCGGTGGAGAATGAGATATGGCACAGCACTaa     <  1:5115281/50‑1 (MQ=255)                                          tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:4728900/1‑48 (MQ=255)                                           gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa    <  1:3564890/49‑1 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:651666/50‑1 (MQ=255)                                                |                                              CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270764‑1270856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑