Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	158.5 / NA	40	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/25);  total (15/25)

GCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742710‑1742800                                              |                                              gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:1522840/1‑50 (MQ=255)  cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          <  1:3341440/50‑1 (MQ=255)   gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:3980927/1‑49 (MQ=255)     cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                       >  1:32199/1‑50 (MQ=255)      aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:89187/50‑1 (MQ=255)         ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   <  1:714725/50‑1 (MQ=255)         ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   >  1:2558745/1‑50 (MQ=255)             ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                <  1:1127320/49‑1 (MQ=255)              tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                               <  1:3218950/49‑1 (MQ=255)                ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             >  1:2053872/1‑49 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           <  1:2106903/49‑1 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                            <  1:1601376/48‑1 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             <  1:4091051/47‑1 (MQ=255)                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         <  1:3099459/50‑1 (MQ=255)                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          <  1:2723690/49‑1 (MQ=255)                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:3957559/50‑1 (MQ=255)                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:4662990/50‑1 (MQ=255)                      agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCCAAAACGCGt                      >  1:2555462/1‑50 (MQ=255)                      agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      <  1:3534571/50‑1 (MQ=255)                      agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      <  1:3194828/50‑1 (MQ=255)                        agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:2998547/50‑1 (MQ=255)                            gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:3652840/1‑50 (MQ=255)                            gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:2386798/1‑50 (MQ=255)                             tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:2485040/1‑49 (MQ=255)                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             <  1:2938468/50‑1 (MQ=255)                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             <  1:2674540/50‑1 (MQ=255)                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             >  1:4350586/1‑50 (MQ=255)                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             <  1:174044/50‑1 (MQ=255)                                gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:1569505/1‑50 (MQ=255)                                gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:2131568/1‑50 (MQ=255)                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           <  1:4665200/50‑1 (MQ=255)                                   ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:543302/49‑1 (MQ=255)                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         <  1:881459/49‑1 (MQ=255)                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc        <  1:3894376/50‑1 (MQ=255)                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:1298871/1‑50 (MQ=255)                                      gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:2945884/1‑49 (MQ=255)                                      gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg      <  1:3612190/50‑1 (MQ=255)                                         tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    <  1:44741/49‑1 (MQ=255)                                          ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  >  1:867891/1‑50 (MQ=255)                                           tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  <  1:2365468/49‑1 (MQ=255)                                              |                                              GCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742710‑1742800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑