Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	284.0 / NA	71	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/42);  total (29/42)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195                                               |                                              cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:5133265/49‑1 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                            >  1:2245985/1‑50 (MQ=255)  gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           <  1:3242399/50‑1 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2662138/1‑49 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:3538179/1‑49 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2191503/1‑49 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:3790147/1‑49 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                          <  1:3070390/50‑1 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                          <  1:3780793/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:1375253/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:3933185/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:4968162/50‑1 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        <  1:842631/50‑1 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        >  1:1740606/1‑50 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                       >  1:2577185/1‑49 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                       <  1:2045677/49‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      <  1:1758238/50‑1 (MQ=255)        ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      >  1:1692415/1‑49 (MQ=255)        ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      >  1:4155502/1‑49 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                     <  1:4741549/48‑1 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   >  1:4133599/1‑50 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   <  1:5051659/49‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   <  1:1570836/49‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  >  1:572992/1‑50 (MQ=255)            agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  >  1:1282400/1‑49 (MQ=255)             gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                 <  1:568437/49‑1 (MQ=255)             gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                <  1:1596903/50‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:1405012/47‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:4593825/47‑1 (MQ=255)                     tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         >  1:2480745/1‑49 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:576249/49‑1 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:4744847/49‑1 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:1161773/49‑1 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:2115913/49‑1 (MQ=255)                       gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:3069795/1‑50 (MQ=255)                       gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                       <  1:3491391/49‑1 (MQ=255)                          ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   >  1:3746901/1‑50 (MQ=255)                           tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:1274919/1‑50 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                     <  1:2514376/45‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:3833487/47‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:4969305/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:213824/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:500201/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:2328432/48‑1 (MQ=255)                              tGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:1927114/1‑50 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                 <  1:1956783/47‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                <  1:3511637/48‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               <  1:4047638/49‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:2445486/1‑49 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:747086/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:4773904/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:740697/50‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:3167082/50‑1 (MQ=255)                                 tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            >  1:1136395/1‑50 (MQ=255)                                 tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            <  1:1604850/50‑1 (MQ=255)                                 tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            >  1:3471015/1‑50 (MQ=255)                                  cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:2153930/1‑50 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:2032462/1‑50 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:840881/1‑50 (MQ=255)                                    gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:210144/1‑49 (MQ=255)                                    gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:4978911/1‑49 (MQ=255)                                    gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:2976331/50‑1 (MQ=255)                                     agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        <  1:3088809/50‑1 (MQ=255)                                      gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       >  1:3942189/1‑50 (MQ=255)                                      gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:3459972/50‑1 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTTATc      >  1:1389727/1‑50 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      <  1:668191/50‑1 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:2046666/50‑1 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     >  1:5006885/1‑50 (MQ=255)                                         tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:1321050/50‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:2332273/49‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  >  1:1460577/1‑50 (MQ=255)                                               |                                              CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑