| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,908,526 | 0 | A | G | 27.4% | 127.0 / 16.1 | 62 | intergenic (‑160/+23) | pstB/pstA | phosphate ABC transporter ATP binding subunit/phosphate ABC transporter membrane subunit PstA |
| Reads supporting (aligned to +/- strand): ref base A (32/13); new base G (0/17); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAA > NC_000913/3908477‑3908574 | aaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa < 1:5304857/50‑1 (MQ=255) aaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg < 1:772486/50‑1 (MQ=255) aGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAgg > 1:1174155/1‑50 (MQ=255) aGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg < 1:2422530/50‑1 (MQ=255) aGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg < 1:2136341/50‑1 (MQ=255) ggCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg < 1:5322885/50‑1 (MQ=255) ggCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg < 1:1995152/50‑1 (MQ=255) ggCGCTCGGTCGGTCACCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg > 1:2367687/1‑50 (MQ=255) gcgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg > 1:2895262/1‑49 (MQ=255) cgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg > 1:4411181/1‑49 (MQ=255) cTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGCG‑aa > 1:4432698/1‑49 (MQ=255) ggtcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAAcc > 1:4269662/1‑50 (MQ=40) ggtcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAAcc > 1:3950943/1‑50 (MQ=40) gtcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCg > 1:1840680/1‑50 (MQ=40) cggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCgtg > 1:6023329/1‑50 (MQ=255) ggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCgtgt > 1:5966340/1‑50 (MQ=255) gtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCgtgt > 1:2946545/1‑49 (MQ=255) tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCaa > 1:1824762/1‑49 (MQ=255) ccGCCCCTTGGGGGGGGGGGTTGGGGGGGGGGG‑AAAACCGTGTCAGCAat < 1:6756278/49‑1 (MQ=12) cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAata > 1:6691655/1‑50 (MQ=255) gCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAatat > 1:5564766/1‑50 (MQ=255) gCCCCTCTGGGG‑GGGGGTTGGGGGGGGGGGAAAAACCGTGTCAGCAatat < 1:1818865/50‑1 (MQ=12) gCCCCTCGGGGGAGGGGGTTGGGGGGGGGGG‑AAAACCGTGTCAGCAatat < 1:1512445/50‑1 (MQ=21) ccccTGGGGGGAGAGGGTTGGGGTGGGGGG‑AAAACCGTGTCAGCAATATc < 1:6867385/50‑1 (MQ=21) cccTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATc > 1:700110/1‑49 (MQ=255) cccTCGGGGGGGAGGTTTAGGGTGGGGGG‑AAAACCGTGTCAGCAATATCa < 1:5305188/50‑1 (MQ=37) cccTCGGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCa < 1:4838164/50‑1 (MQ=255) cccTCGGGGAAGAGGGTGGGGGAGGGGGA‑AAAACCGTGTCAGCAATATCa < 1:2120252/50‑1 (MQ=255) cttctGGGGAGGGGTTTAGGGGGAGGGG‑AAAACCGTGTCAGCAATATCaa < 1:5741696/48‑1 (MQ=25) ctctGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAAc > 1:1243778/1‑50 (MQ=255) ctcgGGGGGGGGGGTAGGGGTGGGGGG‑AAAACCGTGTCAGCAATATCAAc < 1:4927742/50‑1 (MQ=255) tctGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAAcc > 1:921158/1‑50 (MQ=255) tGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCgt > 1:2362623/1‑50 (MQ=255) gggggAGGGGGTGGGGGGGGGGGG‑AAAACCGTGTCAGCAATATCAACCgt < 1:4303276/49‑1 (MQ=255) gggggAGAGGGTTGGGGTGGGGGG‑AAAACCGTGTCAGCAATATCAACCg < 1:5415176/48‑1 (MQ=37) gggAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCgtgt > 1:2373689/1‑50 (MQ=255) gggAGAGGGTAGGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCgtg < 1:2466003/49‑1 (MQ=37) ggAGGGGTTTGGGGTGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGtt < 1:4888258/50‑1 (MQ=255) ggAGAGGGTTGGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGtt < 1:593756/50‑1 (MQ=255) ggAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGtt > 1:5783674/1‑50 (MQ=255) ggAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGtt > 1:3744899/1‑50 (MQ=255) gagaGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGtt > 1:4474224/1‑49 (MQ=255) gagaGGGTAAGGGGGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGttt < 1:5834866/50‑1 (MQ=38) agagGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTa > 1:7281831/1‑50 (MQ=255) agagGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTa > 1:7450684/1‑50 (MQ=255) agagGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTa > 1:1292278/1‑50 (MQ=255) agagGGTGGGGGGGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTa < 1:5832734/50‑1 (MQ=255) gagGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTAt > 1:5138177/1‑50 (MQ=255) gggTTAGGGGGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTAttc < 1:1448772/50‑1 (MQ=255) ggTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTAttct > 1:6738654/1‑50 (MQ=255) ggTGAGGGGGAGGGA‑AAAACCGTGTCAGCAATATCAACCGTGTTTAttct < 1:5415071/50‑1 (MQ=255) gTTGGGGTGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTAttctt < 1:3480655/50‑1 (MQ=255) gTTGGGGTGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTAttctt < 1:1347526/50‑1 (MQ=255) tAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg > 1:3223357/1‑50 (MQ=255) tAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg > 1:2974169/1‑50 (MQ=255) ggggTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg < 1:561749/48‑1 (MQ=255) aGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg > 1:2369542/1‑49 (MQ=255) gggTGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCa < 1:4711295/50‑1 (MQ=255) ggTGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaa < 1:418770/50‑1 (MQ=255) ggTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCa > 1:2494984/1‑49 (MQ=255) gTGGGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaa < 1:642722/50‑1 (MQ=255) gTGAGGGA‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCgaa < 1:200673/50‑1 (MQ=255) tGAGGGG‑AAAACCGTGTCAGCAATATCAACTGTGTTTATTCTTCGCaaa > 1:6467316/1‑49 (MQ=255) aGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaaa < 1:2089604/49‑1 (MQ=255) | AAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGG‑AAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAA > NC_000913/3908477‑3908574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |