Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	304.6 / NA	74	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/37);  total (37/37)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545                                                |                                               gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  1:742452/1‑50 (MQ=255) gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  1:6100955/1‑50 (MQ=255) gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  1:2036919/1‑50 (MQ=255)  cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             >  1:4359947/1‑50 (MQ=255)   ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:684487/1‑50 (MQ=255)   ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            <  1:6484716/50‑1 (MQ=255)   ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:5300077/1‑50 (MQ=255)   ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            <  1:248543/50‑1 (MQ=255)     aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:3926948/1‑49 (MQ=255)     aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:5877650/1‑49 (MQ=255)     aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                          <  1:3358266/50‑1 (MQ=255)      aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                          >  1:5736008/1‑49 (MQ=255)       cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        <  1:1993251/50‑1 (MQ=255)        aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                       >  1:166985/1‑50 (MQ=255)        aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                       <  1:956445/50‑1 (MQ=255)         tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:5480276/1‑50 (MQ=255)         tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      <  1:2661196/50‑1 (MQ=255)         tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      <  1:4299887/50‑1 (MQ=255)         tttCGCCTGCACCTTCGGTATCAGATGCGGCAATCAGTGACGTTGAAAcc                                      >  1:5074150/1‑50 (MQ=255)           tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  1:5807298/49‑1 (MQ=255)            cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  1:331004/49‑1 (MQ=255)            cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  1:4384115/49‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                  <  1:3127183/50‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                  <  1:598524/50‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   <  1:4339653/49‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   <  1:6028275/49‑1 (MQ=255)              ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                 <  1:6578141/50‑1 (MQ=255)              ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:2485869/1‑48 (MQ=255)              ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:3327424/1‑48 (MQ=255)               cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:6982399/1‑47 (MQ=255)                 gCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                              >  1:6527250/1‑50 (MQ=255)                 gCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                              >  1:635578/1‑50 (MQ=255)                  cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             <  1:6327531/50‑1 (MQ=255)                  cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             <  1:4958889/50‑1 (MQ=255)                  cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             <  1:1669127/50‑1 (MQ=255)                   aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  1:7020527/50‑1 (MQ=255)                   aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            >  1:664735/1‑50 (MQ=255)                    ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                           >  1:6980308/1‑50 (MQ=255)                    ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                           <  1:7282674/50‑1 (MQ=255)                     cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          >  1:4789923/1‑50 (MQ=255)                     cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          <  1:4209697/50‑1 (MQ=255)                      ttCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         >  1:1068336/1‑50 (MQ=255)                        cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  1:5274724/49‑1 (MQ=255)                        cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  1:1265719/49‑1 (MQ=255)                         ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       <  1:3433575/49‑1 (MQ=255)                         ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      >  1:6968747/1‑50 (MQ=255)                         ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      <  1:6894084/50‑1 (MQ=255)                          gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     <  1:6629876/50‑1 (MQ=255)                            aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:801888/1‑50 (MQ=255)                            aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:6610913/1‑50 (MQ=255)                             tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  <  1:388869/50‑1 (MQ=255)                              cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:4523305/1‑49 (MQ=255)                              cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:7095223/1‑49 (MQ=255)                               aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:1881280/1‑49 (MQ=255)                                gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:3197245/50‑1 (MQ=255)                                gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:877118/1‑48 (MQ=255)                                gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:1506026/1‑48 (MQ=255)                                 aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:7417540/1‑47 (MQ=255)                                 aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              <  1:7034322/50‑1 (MQ=255)                                  tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              >  1:2172353/1‑49 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           >  1:3848119/1‑50 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           >  1:5556392/1‑50 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa            <  1:1968030/49‑1 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa            <  1:6479419/49‑1 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa            <  1:5869051/49‑1 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa            <  1:4245440/49‑1 (MQ=255)                                      gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:4366507/1‑49 (MQ=255)                                         aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:5472372/1‑50 (MQ=255)                                         aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:4585927/1‑50 (MQ=255)                                            aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCa    <  1:7284683/49‑1 (MQ=255)                                            aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:4582690/50‑1 (MQ=255)                                            aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:1084741/50‑1 (MQ=255)                                             gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:4603294/50‑1 (MQ=255)                                             gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  >  1:3197914/1‑50 (MQ=255)                                                |                                               GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑