| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,270,811 | C→T | V347M (GTG→ATG) | chaA ← | Na(+)/K(+):H(+) antiporter ChaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,270,811 | 0 | C | T | 99.0% | 394.5 / ‑5.9 | 98 | V347M (GTG→ATG) | chaA | Na(+)/K(+):H(+) antiporter ChaA |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (50/47); total (50/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG > NC_000913/1270764‑1270857 | catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt > 1:243219/1‑50 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt < 1:6078524/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt < 1:5497251/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt < 1:5126257/49‑1 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa < 1:6663284/50‑1 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa < 1:6634911/50‑1 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa < 1:6045456/50‑1 (MQ=255) caAATAGGCGGCAAACAGTGCCAGACGCGCCGCGCCATTGAGCATGTTAg > 1:2000197/1‑50 (MQ=38) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg < 1:6842778/49‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg < 1:4770183/49‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:5014645/1‑49 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:5719533/1‑49 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:5871657/1‑49 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt < 1:5196241/50‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt < 1:1266102/50‑1 (MQ=255) aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa > 1:1284205/1‑50 (MQ=255) aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa > 1:4966512/1‑50 (MQ=255) aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc < 1:1029277/50‑1 (MQ=255) aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc > 1:4470399/1‑50 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg > 1:1506671/1‑50 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg < 1:4938304/50‑1 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg < 1:4570935/50‑1 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg > 1:3034364/1‑50 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg < 1:6947753/50‑1 (MQ=255) aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt > 1:358693/1‑50 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc > 1:1782158/1‑50 (MQ=255) gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc < 1:7005199/50‑1 (MQ=255) gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt > 1:4424267/1‑50 (MQ=255) gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt < 1:6054857/50‑1 (MQ=255) gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt < 1:512152/50‑1 (MQ=255) cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:3354990/1‑50 (MQ=255) cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:7200192/1‑50 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg < 1:1017135/50‑1 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:4282648/1‑49 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:5277089/1‑49 (MQ=255) aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:1687556/1‑48 (MQ=255) aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:301153/1‑48 (MQ=255) aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga < 1:4868786/50‑1 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag < 1:7399434/50‑1 (MQ=255) aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag > 1:6784552/1‑48 (MQ=255) aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa < 1:4765944/50‑1 (MQ=255) gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg < 1:7424284/50‑1 (MQ=255) gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:1339021/1‑48 (MQ=255) gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:2951186/1‑48 (MQ=255) ccAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGgag < 1:7221778/50‑1 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:1063442/1‑46 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:1102072/1‑46 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:2459148/1‑46 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga < 1:2174997/50‑1 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga > 1:4831419/1‑49 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga > 1:6867412/1‑49 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat < 1:3668621/50‑1 (MQ=255) aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat > 1:4211808/1‑50 (MQ=255) aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat > 1:2737141/1‑50 (MQ=255) gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg > 1:7004702/1‑50 (MQ=255) cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg > 1:5286172/1‑49 (MQ=255) cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc < 1:4933395/50‑1 (MQ=255) cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc < 1:5407225/50‑1 (MQ=255) cgctgcgcCATTGAGCATGTTAATATGTCCGGTGGAGAAGGAGATATGGc < 1:958213/50‑1 (MQ=255) gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca < 1:2216786/50‑1 (MQ=255) ctgcgcCATTGAGCGTGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:3681231/1‑49 (MQ=255) ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac < 1:5627748/50‑1 (MQ=255) ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac < 1:6092832/50‑1 (MQ=255) ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:789205/1‑49 (MQ=255) ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:3159808/1‑49 (MQ=255) ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:2154607/1‑49 (MQ=255) ctgcgcCATTGAGCATATTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:5749540/1‑49 (MQ=38) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:1474367/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:6784129/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:4416924/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg < 1:2647382/49‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg < 1:402948/49‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 1:4254905/1‑49 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 1:5808625/1‑49 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc < 1:3023737/50‑1 (MQ=255) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa > 1:5265755/1‑50 (MQ=255) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa < 1:94954/50‑1 (MQ=255) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa > 1:5247000/1‑50 (MQ=255) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa > 1:7349957/1‑50 (MQ=255) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa < 1:7311518/50‑1 (MQ=255) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa > 1:3530046/1‑50 (MQ=255) ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc > 1:6770742/1‑50 (MQ=255) ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc < 1:7418162/50‑1 (MQ=255) ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc > 1:2412161/1‑50 (MQ=255) cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc > 1:6847322/1‑49 (MQ=255) aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt > 1:1903270/1‑49 (MQ=255) aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt > 1:4293107/1‑49 (MQ=255) aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa < 1:4411741/50‑1 (MQ=255) ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa < 1:6938855/50‑1 (MQ=255) ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa < 1:6829613/50‑1 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa < 1:5118678/49‑1 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa < 1:3961062/49‑1 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa < 1:6475091/49‑1 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa > 1:5216409/1‑47 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa > 1:6307948/1‑47 (MQ=255) aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag < 1:4986103/49‑1 (MQ=255) gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag > 1:6408870/1‑50 (MQ=255) gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag < 1:3390591/50‑1 (MQ=255) | CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG > NC_000913/1270764‑1270857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |