Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	344.9 / NA	84	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (38/46);  total (38/46)

GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801                                                |                                               gTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGa                                              <  1:2704747/50‑1 (MQ=255)   gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:2919107/1‑50 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           <  1:5531038/50‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:4853739/1‑49 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:4417767/1‑49 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            <  1:4563500/49‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:6307274/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:1626154/50‑1 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:6593263/50‑1 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          >  1:1391468/1‑49 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          >  1:77981/1‑49 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        <  1:5602517/50‑1 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:3781688/1‑50 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       >  1:4639534/1‑50 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       >  1:5100661/1‑50 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       <  1:6513183/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      <  1:6008199/50‑1 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                     <  1:6303623/50‑1 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:4168304/1‑50 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    <  1:854311/50‑1 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGCGTTTCCCGAATTAAATGGa                                    >  1:2482889/1‑50 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:1228283/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:4221777/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:906479/1‑50 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  <  1:3515192/49‑1 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                 <  1:4673081/49‑1 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                 >  1:2631941/1‑49 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                >  1:5360238/1‑49 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:7471944/49‑1 (MQ=255)                 cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               >  1:200333/1‑49 (MQ=255)                 cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               >  1:6975347/1‑49 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            <  1:2939421/49‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                             <  1:4706029/48‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                             <  1:325172/48‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              <  1:1470121/47‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              <  1:5062257/47‑1 (MQ=255)                    cccAAGAGTCATGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              <  1:1777184/47‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:4720492/49‑1 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:4172048/1‑50 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:3129042/1‑50 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:4736904/1‑50 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:6628571/1‑50 (MQ=255)                        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                       <  1:4344414/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                      <  1:5116851/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                      <  1:2781165/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:2274939/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:6593130/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:4057482/50‑1 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    <  1:4231728/50‑1 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    >  1:2697464/1‑50 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:4518494/1‑50 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:1976432/1‑50 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:6160293/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:5672628/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:2608526/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 <  1:34202/50‑1 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:4727053/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:5833819/1‑50 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg                <  1:2252114/50‑1 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg                <  1:4082833/50‑1 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg                <  1:3857671/50‑1 (MQ=255)                                gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa               >  1:5021449/1‑50 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa               <  1:2033348/49‑1 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              >  1:5576702/1‑50 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              <  1:282730/50‑1 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              <  1:5660297/50‑1 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             >  1:2025818/1‑50 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:273249/50‑1 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            <  1:4605532/50‑1 (MQ=255)                                    tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           >  1:6733226/1‑50 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:92121/49‑1 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:3527272/49‑1 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:1410531/49‑1 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg          <  1:1520015/49‑1 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         >  1:6293809/1‑50 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         >  1:4454684/1‑50 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         <  1:4004729/50‑1 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         <  1:763160/50‑1 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         >  1:1132788/1‑50 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        <  1:5287411/50‑1 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      >  1:2293129/1‑50 (MQ=255)                                           tttCCCTAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:4419093/50‑1 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:5530179/1‑50 (MQ=255)                                             tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt  >  1:3988089/1‑50 (MQ=255)                                                |                                               GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑