| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,440,194 | C→A | G279V (GGT→GTT) | rpoA ← | RNA polymerase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,440,194 | 0 | C | A | 80.8% | 262.4 / 64.6 | 104 | G279V (GGT→GTT) | rpoA | RNA polymerase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (9/11); new base A (46/38); total (55/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 105 total aligned reads displayed.
TTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGT > NC_000913/3440149‑3440243 | ttAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGa > 1:7386721/1‑49 (MQ=255) tAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata > 1:4737971/1‑50 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTTCCAGATCACCGata < 1:3982944/49‑1 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGata < 1:642141/49‑1 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata < 1:4289840/49‑1 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata > 1:561102/1‑49 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGatat < 1:5089900/49‑1 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata > 1:5012624/1‑48 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGAtata < 1:7433305/50‑1 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGAtata < 1:5776539/50‑1 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGAtata < 1:7090095/50‑1 (MQ=255) gCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAg < 1:4644914/50‑1 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTg > 1:780459/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:1119441/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:4782135/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg < 1:3101402/50‑1 (MQ=255) ttttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTgg < 1:3976191/50‑1 (MQ=255) tttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa > 1:7197372/1‑50 (MQ=255) tttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa > 1:4808084/1‑50 (MQ=255) ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGa < 1:690038/49‑1 (MQ=255) ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa < 1:1821217/49‑1 (MQ=255) tAAGGGGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGa > 1:2079653/1‑48 (MQ=255) tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGAt < 1:505002/49‑1 (MQ=255) aaGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATa < 1:4052779/49‑1 (MQ=255) aaGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATa < 1:6396261/49‑1 (MQ=255) aaGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAg > 1:34076/1‑50 (MQ=255) aaGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAg > 1:3070744/1‑50 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGAGGATAGc > 1:4742292/1‑50 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAg < 1:2907466/49‑1 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGc < 1:7163088/50‑1 (MQ=255) ggAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCt > 1:1976996/1‑50 (MQ=255) ggAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCt < 1:1422194/50‑1 (MQ=255) ggAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCt < 1:7372213/50‑1 (MQ=255) gAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt < 1:3249438/50‑1 (MQ=255) gAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCtt > 1:472136/1‑50 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc < 1:2603153/50‑1 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc > 1:1839211/1‑50 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc > 1:5529286/1‑50 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc > 1:1753583/1‑50 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:1617286/50‑1 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:5392403/50‑1 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:706372/50‑1 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:3383779/50‑1 (MQ=255) cTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTg > 1:7029935/1‑50 (MQ=255) cTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTg > 1:1567323/1‑50 (MQ=255) tCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc > 1:7247029/1‑50 (MQ=255) tCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc > 1:1769931/1‑50 (MQ=255) cAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCt > 1:1109581/1‑50 (MQ=255) cAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCt > 1:2496578/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt > 1:6092474/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt < 1:3234485/50‑1 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt > 1:6320884/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt > 1:5308452/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCt < 1:817962/49‑1 (MQ=255) aCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCttt < 1:6480250/50‑1 (MQ=255) aCCTCGGTACGCTGTACCAGATCAACGATATAGCGGATAGCTTCTGCtt > 1:3351489/1‑49 (MQ=39) ccTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCttt > 1:686975/1‑49 (MQ=255) ccTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTa < 1:7124656/50‑1 (MQ=255) cTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCttt > 1:6862574/1‑48 (MQ=255) cTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCttt > 1:311748/1‑48 (MQ=255) tCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAg > 1:5572453/1‑50 (MQ=255) cGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg < 1:5311768/50‑1 (MQ=255) ggTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAgg < 1:3312137/49‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg < 1:6788705/49‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg < 1:2634644/49‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg < 1:6285663/49‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:6094225/1‑49 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:4393776/1‑49 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAg < 1:6060057/48‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGc < 1:6886715/50‑1 (MQ=255) gTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa < 1:1131683/50‑1 (MQ=255) gTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:6159799/1‑48 (MQ=255) gTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:5737798/1‑48 (MQ=255) gTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCa < 1:4918492/50‑1 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg > 1:6075565/1‑50 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg > 1:1217960/1‑50 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg > 1:5712204/1‑50 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg > 1:2517688/1‑50 (MQ=255) cGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt < 1:5757786/49‑1 (MQ=255) cGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt < 1:1777534/49‑1 (MQ=255) gCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt > 1:7014260/1‑48 (MQ=255) gCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTa < 1:164170/50‑1 (MQ=255) cTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg < 1:637472/50‑1 (MQ=255) cTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg < 1:3750078/50‑1 (MQ=255) gTACCAGATCACCGATATAGCGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:3598890/1‑48 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGc < 1:1000697/48‑1 (MQ=255) aCCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCa > 1:3012358/1‑48 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag > 1:46322/1‑50 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag > 1:346115/1‑50 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga < 1:1939728/49‑1 (MQ=255) cAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc > 1:6274128/1‑50 (MQ=255) cAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc < 1:3727881/50‑1 (MQ=255) cAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc > 1:4983438/1‑50 (MQ=255) aGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg > 1:84688/1‑50 (MQ=255) aTCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa > 1:2938916/1‑50 (MQ=255) aTCAACGATATAGCGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa > 1:5621308/1‑50 (MQ=255) tCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAc < 1:5630130/50‑1 (MQ=255) tCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa > 1:2047377/1‑49 (MQ=255) tCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa > 1:4744272/1‑49 (MQ=255) ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGt > 1:916458/1‑50 (MQ=255) | TTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGT > NC_000913/3440149‑3440243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |