Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,477,150 C→T K90K (AAG→AAA)  fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,1500CT100.0% 439.9 / NA 107K90K (AAG→AAA) fkpApeptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/64);  total (43/64)

Only 100 of 107 total aligned reads displayed.

GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477105‑3477195
                                             |                                             
gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:1274293/1‑50 (MQ=255)
gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           <  1:1642644/50‑1 (MQ=255)
gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:526484/1‑50 (MQ=255)
 aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2181305/1‑49 (MQ=255)
 aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                          <  1:7351771/50‑1 (MQ=255)
  aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2212229/1‑48 (MQ=255)
  aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:5272248/50‑1 (MQ=255)
    gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        >  1:4705701/1‑49 (MQ=255)
     cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                       >  1:1754054/1‑49 (MQ=255)
      ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGCGTTTGCTTTTATCAGCa                                      >  1:6027047/1‑49 (MQ=255)
      ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      >  1:3389362/1‑49 (MQ=255)
      ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      >  1:1512560/1‑49 (MQ=255)
        gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    <  1:4092487/49‑1 (MQ=255)
        gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    <  1:6548171/49‑1 (MQ=255)
        gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                     <  1:1833552/48‑1 (MQ=255)
        gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   >  1:2506574/1‑50 (MQ=255)
         tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   <  1:1314110/49‑1 (MQ=255)
          agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  >  1:1041742/1‑49 (MQ=255)
           gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                 <  1:741801/49‑1 (MQ=255)
           gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                <  1:5411060/50‑1 (MQ=255)
            agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               >  1:5167053/1‑50 (MQ=255)
            agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               >  1:1892682/1‑50 (MQ=255)
            agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               <  1:4278899/50‑1 (MQ=255)
            agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               <  1:1296550/50‑1 (MQ=255)
            agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               >  1:3142055/1‑50 (MQ=255)
            agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               <  1:3068528/50‑1 (MQ=255)
             gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                              <  1:6879208/50‑1 (MQ=255)
             gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                              >  1:3879467/1‑50 (MQ=255)
             gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                              <  1:2353760/50‑1 (MQ=255)
              tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:5864722/50‑1 (MQ=255)
              tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:387829/50‑1 (MQ=255)
               cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            >  1:6285018/1‑50 (MQ=255)
                 gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           >  1:1628044/1‑49 (MQ=255)
                  ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:2432735/47‑1 (MQ=255)
                  ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:6978460/50‑1 (MQ=255)
                  ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:2030418/50‑1 (MQ=255)
                    cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:2775941/49‑1 (MQ=255)
                    cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:1642867/49‑1 (MQ=255)
                     gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:6423058/1‑50 (MQ=255)
                     gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:5246147/1‑50 (MQ=255)
                     gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                       <  1:1351226/49‑1 (MQ=255)
                       tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:2701877/1‑48 (MQ=255)
                       tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:373390/1‑48 (MQ=255)
                        ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   >  1:2419885/1‑50 (MQ=255)
                          ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:5892639/1‑49 (MQ=255)
                          ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:3131333/1‑49 (MQ=255)
                          ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:6675580/1‑49 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      <  1:5019143/44‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACGCCAg                   <  1:1205029/47‑1 (MQ=39)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:6313305/46‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:6581870/46‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:6754191/46‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:6963129/46‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:3870934/46‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:31694/47‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:5417650/47‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:6132025/48‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:2380599/48‑1 (MQ=255)
                           ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:3823813/48‑1 (MQ=255)
                             ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                 <  1:6189555/47‑1 (MQ=255)
                             ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:2762902/1‑49 (MQ=255)
                              gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:386044/1‑48 (MQ=255)
                              gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:6468110/1‑48 (MQ=255)
                              gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:5380204/49‑1 (MQ=255)
                              gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:601095/49‑1 (MQ=255)
                              gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:1742632/49‑1 (MQ=255)
                              gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:2102933/50‑1 (MQ=255)
                               tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            <  1:4773639/50‑1 (MQ=255)
                                cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:3817710/1‑50 (MQ=255)
                                 ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAATACCAGCGATCAGCt          >  1:6294331/1‑50 (MQ=255)
                                 ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           <  1:1156932/49‑1 (MQ=255)
                                 ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           <  1:7170398/49‑1 (MQ=255)
                                 ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           <  1:2146221/49‑1 (MQ=255)
                                 ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:1866122/50‑1 (MQ=255)
                                 ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:5035102/50‑1 (MQ=255)
                                  gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:7144486/1‑49 (MQ=255)
                                  gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:3226227/50‑1 (MQ=255)
                                  gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:4443487/50‑1 (MQ=255)
                                  gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:7344213/50‑1 (MQ=255)
                                   agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        >  1:234867/1‑50 (MQ=255)
                                    gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:73190/50‑1 (MQ=255)
                                    gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       >  1:5638718/1‑50 (MQ=255)
                                    gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:1787413/50‑1 (MQ=255)
                                    gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:5055835/50‑1 (MQ=255)
                                     agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      >  1:1927373/1‑50 (MQ=255)
                                      gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     >  1:1489384/1‑50 (MQ=255)
                                      gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:2324465/50‑1 (MQ=255)
                                      gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:3042373/50‑1 (MQ=255)
                                       tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:3797155/50‑1 (MQ=255)
                                       tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:6042660/50‑1 (MQ=255)
                                       tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:3919926/50‑1 (MQ=255)
                                       tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    >  1:7241881/1‑50 (MQ=255)
                                       tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    >  1:5217368/1‑50 (MQ=255)
                                       tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    >  1:3676045/1‑50 (MQ=255)
                                         tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:430916/49‑1 (MQ=255)
                                         tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:2313884/49‑1 (MQ=255)
                                         tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  >  1:542242/1‑50 (MQ=255)
                                         tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  >  1:635708/1‑50 (MQ=255)
                                          gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  <  1:4529092/49‑1 (MQ=255)
                                          gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  <  1:145732/49‑1 (MQ=255)
                                             |                                             
GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477105‑3477195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑