Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,151,686 | 0 | G | T | 55.9% | ‑4.3 / 19.3 | 35 | intergenic (+40/+25) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
Reads supporting (aligned to +/- strand): ref base G (11/4); new base T (6/13); total (18/17) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.66e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-02 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC > NC_000913/2151448‑2151914 | tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑ttg < 2:1504018/239‑1 (MQ=255) tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑ttg < 1:224474/239‑1 (MQ=255) gTTAAAAACGTAGAAGATGGCGGGGAGTGGAAAATAAAAAAAGAAGATATTACAGACGGGATGTATAAGGGGGGAGATAAAATGTAC‑GGCGTTTATACGTTTGATCTTTTATGGCTCACCTTACAAAAAAAGGAACCGGACGAGAAAAGAGAAAAATTATTTCGCAAATCGGCCTTTATATTTTTTGTTAAAG‑CGGGGGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCActct < 2:1559765/239‑1 (MQ=255) aCAAAGGAAGAATAGGGCAGGGGGGGAAAAAACAAAAAAGAAGTTATTTCAGATTGGTTGTTTCCG‑GGGGGGAAAAAAATTACGGGCGTTTATACAATTGATCTTTTATTGGTCCCCAACAAAAAAGAAGAAGCGGACGAGCAAAGAAAAAAATTAGTTCGATAATCCGCCTTTATATTTTTTTTTAAAA‑CGGGGGG‑TGAAA‑TTGTTGTTTT‑AAA‑CCGCCATGAGAAACTCACTCTGcc < 2:1158713/239‑1 (MQ=255) aaTATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑ttg < 1:61952/195‑1 (MQ=255) aaTAAAATTCAC‑GGCTGTTAAACCATTGCTTCTTTTTTGCTCACCAACCAAAAAAAAAGACCTCACGAAGAAAAAAAAATATTCTTTCCTTAACCGCTCGTTAT‑TTTATTGTTAAAA‑ACGGGGG‑GTAAA‑TTTTTTTCTA‑AAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCttt < 2:1776666/238‑1 (MQ=255) tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGCACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTaa > 1:1545306/1‑215 (MQ=255) ggTCACCTATCCAAAAGAAGAAGCCGACGAGCAAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑CTGAA‑ATCTTTTTTT‑TAA‑CCGCCCCGAGAAACTCCCTCTGCCCACAACGCCAAGGTCAACGTTTTATCATCATCCGTACCGCCGTTTATCCCCCCGCGGGACAAAAACTACTTTAACCAATACGGTAATAAATAGAGTTGTTCCTGCggt > 2:1476080/1‑235 (MQ=255) gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑ttg < 1:1662319/94‑1 (MQ=255) gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑TAA‑CCGCCATGGGAAACATACTCTGCCCACAACGCCAGTGCCAACGTTTTTTTATTATCCGTACGCTCCTTTACCCCCGGGCTGGACAAAAAATGGTTTTACAAATTAGGGAATAAATAGAGGTTTTCTTGCggt > 2:74368/1‑234 (MQ=255) tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTTAA‑TTTTTTTCTT‑TAA‑ACGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCCTCCGTa > 1:1296111/1‑163 (MQ=255) cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGgaat > 1:1431806/1‑213 (MQ=255) cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTTAA‑TTGTTGTCTT‑TAA‑ACCCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGGTTTatc > 1:2283729/1‑149 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGc > 1:266051/1‑160 (MQ=255) tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAACGCCAGTGCCCACGGTTTATTATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTTAGGTAATAAATCGAGTTGTTCCTTCGTTGCTggcg > 2:668791/1‑235 (MQ=255) gaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTTTTTTCTT‑CCA‑CCCCCATGAGAAAATAACTCCGCCCCCAGCGCCAGTGCCAAAGTTTTTTCAACATTCCCACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTg > 2:844209/1‑239 (MQ=255) aagaagCCGACGAGCTAAGAAAAAAATTATTTCGAAAACCGGCGGTTATATTTTTTTTTTAAC‑CGGGCGG‑TTAAA‑TTTTTTTTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc < 1:1989580/127‑1 (MQ=255) gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑ATGAA‑TTTTTGTCTT‑TCA‑CCCCCATGAGAAAATCACCCTGCCCACCGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGTTTAACGCCGGGCTGGACAAAAAATGCTTTAAACAATCAGGTAATAAAACGAGTTGTTTCTTCGTTGCTTCCGCCAGGCCCTTGaaa > 2:368247/1‑239 (MQ=255) gaaaaGAGAAAATTTATTTCGTTAATCAGCCTTTATTTTTTTTTTTAAAC‑CGGGGGG‑TTAAT‑TTTTTTTCTA‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc < 1:1589569/151‑1 (MQ=255) agCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTTAA‑ATTTTGTTTT‑CAA‑CCCCCCTGAGAAAATCACCCTGCCCACAGCCCCCCAGCCCACGGTTTATCATCATCCGGACGCCCGTTTTCCCCCCGGGGGGGCAAAAACTGCTTTAACAAAATAAGGAAAAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTTCCATGAAAAACGGGGTaaagaaaag > 2:984511/1‑237 (MQ=255) aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTTTTTTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCCCAGCGCCCGTGCCCACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCCGGCTGGACaaaaa > 2:1589569/1‑154 (MQ=255) aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑ATTTTTTTTT‑CAA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCCTTTACCCCCCGGCTGGACAAAAAATGCCTTTACAATTTAGGTAATAAATTGAGTTGTTCCTGCGTTGCTGCCGCCCGGCCATTTAAAAAAGGGGGAAAAAAAAGcaca > 2:236770/1‑239 (MQ=255) aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑CCA‑CCCCCCTGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACCCTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCCGGACAAAAACTGCTTTTACCATTCAGGTAATAAATCCAGTTTTTCCTGCGTTTCTGCCGCCCGACCATTGAAAAACGGGGGTAAAAAAGGCACAt > 2:67780/1‑239 (MQ=255) cgtgttgttttATTTTTTTTTTTTAAT‑GTGGGGT‑TTAAT‑TTTTTTTCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt < 1:712247/230‑1 (MQ=255) gcttttATATTT‑TTTTTTAAA‑CCGGGGG‑TTTAATTTTTTGTCTA‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACTTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc < 1:668791/235‑1 (MQ=255) cttttATTTTTTTTGTTTAAG‑TTGGCGT‑TTGAT‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa < 1:1476080/236‑1 (MQ=255) ttttttttttttttAAA‑CTGGGGG‑TTTAA‑TTTTTTTTTTCCAC‑CCGCCATGAGAACCTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCatat < 1:67780/235‑1 (MQ=255) ttttttttGTTTAAC‑CGGGGGT‑TTAAT‑TTTTTGTCTC‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTa < 2:1163411/98‑1 (MQ=255) attttttGTTTAAC‑CGGGGGG‑TGTAT‑TTTTTTTCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTaa < 1:1570536/239‑1 (MQ=255) ttttttAAA‑CTGGGGGTTTGAT‑TTTTTTTCTC‑CAC‑CCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc < 1:984511/236‑1 (MQ=255) tGTTAAAC‑CGGGGGG‑TTGAT‑TTTTTTTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt < 1:23218/78‑1 (MQ=255) tttAAC‑CGGGGGG‑TTGAT‑TTTTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa < 1:621823/71‑1 (MQ=255) tttAAAGCTGGCGT‑TTAAT‑TTGTTTTCTC‑AAA‑CCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt < 1:1931037/239‑1 (MQ=255) ttAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑CAA‑CCGCCCTGAGAAACTCACTCTTCCCCCAGCGCCCGTGCCCACGTTTTATTATCATCCGTACGCTCGTTTACCCCCGGGCTGGGCAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAGAAAAGCACATGAGGAGAATTATCCAGCATATTTAACg > 2:1570536/1‑232 (MQ=255) ttAAA‑CTGGCGG‑TTGAA‑TTGTTGTTTT‑CCA‑CCCCCATGAGAAAATTACCCTTCCCACCGCGGCAGTGGTCAAGTTTTATCATCATCCGTACGCTCGTTTTCCGCCGGGCTGGTCCAAAAATTCTTTAACAATTTCGGTAATAAATCGAGTTTTTCCTGCGTTGTTGCCGCCAGTTCATTCAAAAAAGGGGGAAAGAAAAGCACATGCGGAGAAATATCCAGCACATTTaaagtcaagcg > 2:699109/1‑230 (MQ=255) ttAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTa > 1:1163411/1‑100 (MQ=255) tAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTAAAATTCCggg > 1:1413214/1‑136 (MQ=255) tgg‑TTAAT‑TTTTTTTCTA‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc < 1:2284736/237‑1 (MQ=255) g‑TTTAA‑TTTTTTTCTTACAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc < 1:1335249/238‑1 (MQ=255) | TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC > NC_000913/2151448‑2151914 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |