Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6860GT55.9% ‑4.3 / 19.3 35intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (11/4);  new base T (6/13);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 3.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC  >  NC_000913/2151448‑2151914
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      
tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg                                                                                                                                                                                                                                        <  2:1504018/239‑1 (MQ=255)
tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg                                                                                                                                                                                                                                        <  1:224474/239‑1 (MQ=255)
                               gTTAAAAACGTAGAAGATGGCGGGGAGTGGAAAATAAAAAAAGAAGATATTACAGACGGGATGTATAAGGGGGGAGATAAAATGTACGGCGTTTATACGTTTGATCTTTTATGGCTCACCTTACAAAAAAAGGAACCGGACGAGAAAAGAGAAAAATTATTTCGCAAATCGGCCTTTATATTTTTTGTTAAAGCGGGGGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCActct                                                                                                                                                                                                       <  2:1559765/239‑1 (MQ=255)
                                  aCAAAGGAAGAATAGGGCAGGGGGGGAAAAAACAAAAAAGAAGTTATTTCAGATTGGTTGTTTCCGGGGGGGAAAAAAATTACGGGCGTTTATACAATTGATCTTTTATTGGTCCCCAACAAAAAAGAAGAAGCGGACGAGCAAAGAAAAAAATTAGTTCGATAATCCGCCTTTATATTTTTTTTTAAAACGGGGGGTGAAATTGTTGTTTTAAACCGCCATGAGAAACTCACTCTGcc                                                                                                                                                                                                    <  2:1158713/239‑1 (MQ=255)
                                            aaTATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg                                                                                                                                                                                                                                        <  1:61952/195‑1 (MQ=255)
                                                                                                          aaTAAAATTCACGGCTGTTAAACCATTGCTTCTTTTTTGCTCACCAACCAAAAAAAAAGACCTCACGAAGAAAAAAAAATATTCTTTCCTTAACCGCTCGTTATTTTATTGTTAAAAACGGGGGGTAAATTTTTTTCTAAAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCttt                                                                                                                            <  2:1776666/238‑1 (MQ=255)
                                                                                                                                     tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGCACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTaa                                                                                                                          >  1:1545306/1‑215 (MQ=255)
                                                                                                                                                 ggTCACCTATCCAAAAGAAGAAGCCGACGAGCAAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGCTGAAATCTTTTTTTTAACCGCCCCGAGAAACTCCCTCTGCCCACAACGCCAAGGTCAACGTTTTATCATCATCCGTACCGCCGTTTATCCCCCCGCGGGACAAAAACTACTTTAACCAATACGGTAATAAATAGAGTTGTTCCTGCggt                                                                                        >  2:1476080/1‑235 (MQ=255)
                                                                                                                                                  gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg                                                                                                                                                                                                                                        <  1:1662319/94‑1 (MQ=255)
                                                                                                                                                  gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTTAACCGCCATGGGAAACATACTCTGCCCACAACGCCAGTGCCAACGTTTTTTTATTATCCGTACGCTCCTTTACCCCCGGGCTGGACAAAAAATGGTTTTACAAATTAGGGAATAAATAGAGGTTTTCTTGCggt                                                                                        >  2:74368/1‑234 (MQ=255)
                                                                                                                                                   tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTTTTTTCTTTAAACGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCCTCCGTa                                                                                                                                                                >  1:1296111/1‑163 (MQ=255)
                                                                                                                                                    cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGgaat                                                                                                             >  1:1431806/1‑213 (MQ=255)
                                                                                                                                                       cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTGTTGTCTTTAAACCCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGGTTTatc                                                                                                                                                                          >  1:2283729/1‑149 (MQ=255)
                                                                                                                                                         aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGc                                                                                                                                                             >  1:266051/1‑160 (MQ=255)
                                                                                                                                                          tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAACGCCAGTGCCCACGGTTTATTATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTTAGGTAATAAATCGAGTTGTTCCTTCGTTGCTggcg                                                                                 >  2:668791/1‑235 (MQ=255)
                                                                                                                                                                 gaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTCCACCCCCATGAGAAAATAACTCCGCCCCCAGCGCCAGTGCCAAAGTTTTTTCAACATTCCCACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTg                                                                      >  2:844209/1‑239 (MQ=255)
                                                                                                                                                                  aagaagCCGACGAGCTAAGAAAAAAATTATTTCGAAAACCGGCGGTTATATTTTTTTTTTAACCGGGCGGTTAAATTTTTTTTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc                                                                                                                                                                                     <  1:1989580/127‑1 (MQ=255)
                                                                                                                                                                    gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGATGAATTTTTGTCTTTCACCCCCATGAGAAAATCACCCTGCCCACCGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGTTTAACGCCGGGCTGGACAAAAAATGCTTTAAACAATCAGGTAATAAAACGAGTTGTTTCTTCGTTGCTTCCGCCAGGCCCTTGaaa                                                                   >  2:368247/1‑239 (MQ=255)
                                                                                                                                                                               gaaaaGAGAAAATTTATTTCGTTAATCAGCCTTTATTTTTTTTTTTAAACCGGGGGGTTAATTTTTTTTCTAAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc                                                                                                                                             <  1:1589569/151‑1 (MQ=255)
                                                                                                                                                                                     agCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAAATTTTGTTTTCAACCCCCCTGAGAAAATCACCCTGCCCACAGCCCCCCAGCCCACGGTTTATCATCATCCGGACGCCCGTTTTCCCCCCGGGGGGGCAAAAACTGCTTTAACAAAATAAGGAAAAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTTCCATGAAAAACGGGGTaaagaaaag                                                  >  2:984511/1‑237 (MQ=255)
                                                                                                                                                                                         aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCCCAGCGCCCGTGCCCACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCCGGCTGGACaaaaa                                                                                                                                   >  2:1589569/1‑154 (MQ=255)
                                                                                                                                                                                         aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAATTTTTTTTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCCTTTACCCCCCGGCTGGACAAAAAATGCCTTTACAATTTAGGTAATAAATTGAGTTGTTCCTGCGTTGCTGCCGCCCGGCCATTTAAAAAAGGGGGAAAAAAAAGcaca                                              >  2:236770/1‑239 (MQ=255)
                                                                                                                                                                                          aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCCACCCCCCTGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACCCTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCCGGACAAAAACTGCTTTTACCATTCAGGTAATAAATCCAGTTTTTCCTGCGTTTCTGCCGCCCGACCATTGAAAAACGGGGGTAAAAAAGGCACAt                                             >  2:67780/1‑239 (MQ=255)
                                                                                                                                                                                                      cgtgttgttttATTTTTTTTTTTTAATGTGGGGTTTAATTTTTTTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt                                 <  1:712247/230‑1 (MQ=255)
                                                                                                                                                                                                           gcttttATATTTTTTTTTAAACCGGGGGTTTAATTTTTTGTCTAAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACTTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                            <  1:668791/235‑1 (MQ=255)
                                                                                                                                                                                                            cttttATTTTTTTTGTTTAAGTTGGCGTTTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                           <  1:1476080/236‑1 (MQ=255)
                                                                                                                                                                                                                ttttttttttttttAAACTGGGGGTTTAATTTTTTTTTTCCACCCGCCATGAGAACCTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCatat                        <  1:67780/235‑1 (MQ=255)
                                                                                                                                                                                                                  ttttttttGTTTAACCGGGGGTTTAATTTTTTGTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTa                                                                                                                                                                <  2:1163411/98‑1 (MQ=255)
                                                                                                                                                                                                                   attttttGTTTAACCGGGGGGTGTATTTTTTTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTaa                    <  1:1570536/239‑1 (MQ=255)
                                                                                                                                                                                                                        ttttttAAACTGGGGGTTTGATTTTTTTTCTCCACCCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc                <  1:984511/236‑1 (MQ=255)
                                                                                                                                                                                                                         tGTTAAACCGGGGGGTTGATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                               <  1:23218/78‑1 (MQ=255)
                                                                                                                                                                                                                           tttAACCGGGGGGTTGATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa                                                                                                                                                                                    <  1:621823/71‑1 (MQ=255)
                                                                                                                                                                                                                           tttAAAGCTGGCGTTTAATTTGTTTTCTCAAACCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt             <  1:1931037/239‑1 (MQ=255)
                                                                                                                                                                                                                            ttAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCCTGAGAAACTCACTCTTCCCCCAGCGCCCGTGCCCACGTTTTATTATCATCCGTACGCTCGTTTACCCCCGGGCTGGGCAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAGAAAAGCACATGAGGAGAATTATCCAGCATATTTAACg                  >  2:1570536/1‑232 (MQ=255)
                                                                                                                                                                                                                            ttAAACTGGCGGTTGAATTGTTGTTTTCCACCCCCATGAGAAAATTACCCTTCCCACCGCGGCAGTGGTCAAGTTTTATCATCATCCGTACGCTCGTTTTCCGCCGGGCTGGTCCAAAAATTCTTTAACAATTTCGGTAATAAATCGAGTTTTTCCTGCGTTGTTGCCGCCAGTTCATTCAAAAAAGGGGGAAAGAAAAGCACATGCGGAGAAATATCCAGCACATTTaaagtcaagcg           >  2:699109/1‑230 (MQ=255)
                                                                                                                                                                                                                            ttAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTa                                                                                                                                                      >  1:1163411/1‑100 (MQ=255)
                                                                                                                                                                                                                             tAAACTGGCGGTTGAATTGTTGTCTTCAACCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTAAAATTCCggg                                                                                                                >  1:1413214/1‑136 (MQ=255)
                                                                                                                                                                                                                                      tggTTAATTTTTTTTCTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc   <  1:2284736/237‑1 (MQ=255)
                                                                                                                                                                                                                                        gTTTAATTTTTTTCTTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc  <  1:1335249/238‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      
TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC  >  NC_000913/2151448‑2151914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: