Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6890GT57.1% 6.9 / 14.0 35intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (9/6);  new base T (9/11);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151479‑2151924
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             
gTTAAAAACGTAGAAGATGGCGGGGAGTGGAAAATAAAAAAAGAAGATATTACAGACGGGATGTATAAGGGGGGAGATAAAATGTAC‑GGCGTTTATACGTTTGATCTTTTATGGCTCACCTTACAAAAAAAGGAACCGGACGAGAAAAGAGAAAAATTATTTCGCAAATCGGCCTTTATATTTTTTGTTAAAG‑CGGGGGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCActct                                                                                                                                                                                                                 <  2:1559765/239‑1 (MQ=255)
   aCAAAGGAAGAATAGGGCAGGGGGGGAAAAAACAAAAAAGAAGTTATTTCAGATTGGTTGTTTCCG‑GGGGGGAAAAAAATTACGGGCGTTTATACAATTGATCTTTTATTGGTCCCCAACAAAAAAGAAGAAGCGGACGAGCAAAGAAAAAAATTAGTTCGATAATCCGCCTTTATATTTTTTTTTAAAA‑CGGGGGG‑TGAAA‑TTGTTGTTTT‑AAA‑CCGCCATGAGAAACTCACTCTGcc                                                                                                                                                                                                              <  2:1158713/239‑1 (MQ=255)
                                                                           aaTAAAATTCAC‑GGCTGTTAAACCATTGCTTCTTTTTTGCTCACCAACCAAAAAAAAAGACCTCACGAAGAAAAAAAAATATTCTTTCCTTAACCGCTCGTTAT‑TTTATTGTTAAAA‑ACGGGGG‑GTAAA‑TTTTTTTCTA‑AAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCttt                                                                                                                                      <  2:1776666/238‑1 (MQ=255)
                                                                                                      tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGCACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTaa                                                                                                                                    >  1:1545306/1‑215 (MQ=255)
                                                                                                                  ggTCACCTATCCAAAAGAAGAAGCCGACGAGCAAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑CTGAA‑ATCTTTTTTT‑TAA‑CCGCCCCGAGAAACTCCCTCTGCCCACAACGCCAAGGTCAACGTTTTATCATCATCCGTACCGCCGTTTATCCCCCCGCGGGACAAAAACTACTTTAACCAATACGGTAATAAATAGAGTTGTTCCTGCggt                                                                                                  >  2:1476080/1‑235 (MQ=255)
                                                                                                                   gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑TAA‑CCGCCATGGGAAACATACTCTGCCCACAACGCCAGTGCCAACGTTTTTTTATTATCCGTACGCTCCTTTACCCCCGGGCTGGACAAAAAATGGTTTTACAAATTAGGGAATAAATAGAGGTTTTCTTGCggt                                                                                                  >  2:74368/1‑234 (MQ=255)
                                                                                                                    tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTTAA‑TTTTTTTCTT‑TAA‑ACGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCCTCCGTa                                                                                                                                                                          >  1:1296111/1‑163 (MQ=255)
                                                                                                                     cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGgaat                                                                                                                       >  1:1431806/1‑213 (MQ=255)
                                                                                                                        cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTTAA‑TTGTTGTCTT‑TAA‑ACCCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGGTTTatc                                                                                                                                                                                    >  1:2283729/1‑149 (MQ=255)
                                                                                                                          aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGc                                                                                                                                                                       >  1:266051/1‑160 (MQ=255)
                                                                                                                           tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAACGCCAGTGCCCACGGTTTATTATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTTAGGTAATAAATCGAGTTGTTCCTTCGTTGCTggcg                                                                                           >  2:668791/1‑235 (MQ=255)
                                                                                                                                  gaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTTTTTTCTT‑CCA‑CCCCCATGAGAAAATAACTCCGCCCCCAGCGCCAGTGCCAAAGTTTTTTCAACATTCCCACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTg                                                                                >  2:844209/1‑239 (MQ=255)
                                                                                                                                   aagaagCCGACGAGCTAAGAAAAAAATTATTTCGAAAACCGGCGGTTATATTTTTTTTTTAAC‑CGGGCGG‑TTAAA‑TTTTTTTTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc                                                                                                                                                                                               <  1:1989580/127‑1 (MQ=255)
                                                                                                                                     gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑ATGAA‑TTTTTGTCTT‑TCA‑CCCCCATGAGAAAATCACCCTGCCCACCGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGTTTAACGCCGGGCTGGACAAAAAATGCTTTAAACAATCAGGTAATAAAACGAGTTGTTTCTTCGTTGCTTCCGCCAGGCCCTTGaaa                                                                             >  2:368247/1‑239 (MQ=255)
                                                                                                                                                gaaaaGAGAAAATTTATTTCGTTAATCAGCCTTTATTTTTTTTTTTAAAC‑CGGGGGG‑TTAAT‑TTTTTTTCTA‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc                                                                                                                                                       <  1:1589569/151‑1 (MQ=255)
                                                                                                                                                      agCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTTAA‑ATTTTGTTTT‑CAA‑CCCCCCTGAGAAAATCACCCTGCCCACAGCCCCCCAGCCCACGGTTTATCATCATCCGGACGCCCGTTTTCCCCCCGGGGGGGCAAAAACTGCTTTAACAAAATAAGGAAAAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTTCCATGAAAAACGGGGTaaagaaaag                                                            >  2:984511/1‑237 (MQ=255)
                                                                                                                                                          aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTTTTTTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCCCAGCGCCCGTGCCCACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCCGGCTGGACaaaaa                                                                                                                                             >  2:1589569/1‑154 (MQ=255)
                                                                                                                                                          aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑ATTTTTTTTT‑CAA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCCTTTACCCCCCGGCTGGACAAAAAATGCCTTTACAATTTAGGTAATAAATTGAGTTGTTCCTGCGTTGCTGCCGCCCGGCCATTTAAAAAAGGGGGAAAAAAAAGcaca                                                        >  2:236770/1‑239 (MQ=255)
                                                                                                                                                           aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑CCA‑CCCCCCTGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACCCTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCCGGACAAAAACTGCTTTTACCATTCAGGTAATAAATCCAGTTTTTCCTGCGTTTCTGCCGCCCGACCATTGAAAAACGGGGGTAAAAAAGGCACAt                                                       >  2:67780/1‑239 (MQ=255)
                                                                                                                                                                       cgtgttgttttATTTTTTTTTTTTAAT‑GTGGGGT‑TTAAT‑TTTTTTTCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt                                           <  1:712247/230‑1 (MQ=255)
                                                                                                                                                                            gcttttATATTT‑TTTTTTAAA‑CCGGGGG‑TTTAATTTTTTGTCTA‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACTTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                      <  1:668791/235‑1 (MQ=255)
                                                                                                                                                                             cttttATTTTTTTTGTTTAAG‑TTGGCGT‑TTGAT‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                     <  1:1476080/236‑1 (MQ=255)
                                                                                                                                                                                 ttttttttttttttAAA‑CTGGGGG‑TTTAA‑TTTTTTTTTTCCAC‑CCGCCATGAGAACCTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCatat                                  <  1:67780/235‑1 (MQ=255)
                                                                                                                                                                                   ttttttttGTTTAAC‑CGGGGGT‑TTAAT‑TTTTTGTCTC‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTa                                                                                                                                                                          <  2:1163411/98‑1 (MQ=255)
                                                                                                                                                                                    attttttGTTTAAC‑CGGGGGG‑TGTAT‑TTTTTTTCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTaa                              <  1:1570536/239‑1 (MQ=255)
                                                                                                                                                                                         ttttttAAA‑CTGGGGGTTTGAT‑TTTTTTTCTC‑CAC‑CCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc                          <  1:984511/236‑1 (MQ=255)
                                                                                                                                                                                          tGTTAAAC‑CGGGGGG‑TTGAT‑TTTTTTTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                                         <  1:23218/78‑1 (MQ=255)
                                                                                                                                                                                            tttAAC‑CGGGGGG‑TTGAT‑TTTTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa                                                                                                                                                                                              <  1:621823/71‑1 (MQ=255)
                                                                                                                                                                                            tttAAAGCTGGCGT‑TTAAT‑TTGTTTTCTC‑AAA‑CCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt                       <  1:1931037/239‑1 (MQ=255)
                                                                                                                                                                                             ttAAA‑CTGGCGG‑TTGAA‑TTGTTTTCTT‑CAA‑CCGCCCTGAGAAACTCACTCTTCCCCCAGCGCCCGTGCCCACGTTTTATTATCATCCGTACGCTCGTTTACCCCCGGGCTGGGCAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAGAAAAGCACATGAGGAGAATTATCCAGCATATTTAACg                            >  2:1570536/1‑232 (MQ=255)
                                                                                                                                                                                             ttAAA‑CTGGCGG‑TTGAA‑TTGTTGTTTT‑CCA‑CCCCCATGAGAAAATTACCCTTCCCACCGCGGCAGTGGTCAAGTTTTATCATCATCCGTACGCTCGTTTTCCGCCGGGCTGGTCCAAAAATTCTTTAACAATTTCGGTAATAAATCGAGTTTTTCCTGCGTTGTTGCCGCCAGTTCATTCAAAAAAGGGGGAAAGAAAAGCACATGCGGAGAAATATCCAGCACATTTaaagtcaagcg                     >  2:699109/1‑230 (MQ=255)
                                                                                                                                                                                             ttAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTa                                                                                                                                                                >  1:1163411/1‑100 (MQ=255)
                                                                                                                                                                                              tAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTAAAATTCCggg                                                                                                                          >  1:1413214/1‑136 (MQ=255)
                                                                                                                                                                                                       tgg‑TTAAT‑TTTTTTTCTA‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc             <  1:2284736/237‑1 (MQ=255)
                                                                                                                                                                                                         g‑TTTAA‑TTTTTTTCTTACAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc            <  1:1335249/238‑1 (MQ=255)
                                                                                                                                                                                                                      gtCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  2:1119723/1‑236 (MQ=255)
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             
GTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGG‑TTGAA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151479‑2151924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑