Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,473	0	T	G	31.6%	185.6 / 14.6	76	G154G (GGT→GGG)	prfA	peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base T (12/40);  new base G (24/0);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 3.32e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCG  >  NC_000913/1265244‑1265698                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     cgacggATGAACTGCGCGAAGCTAAAGAAAAGAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGACCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTTTTCCGTATGTACGGCCGTTATGCGGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACAtgg                                                                                                                                                                                                                              <  2:506202/234‑1 (MQ=255)      gATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGGAATCATGAGGGCCAGCGAGGGGGGCCATgggggtga                                                                                                                                                                                                                        >  2:35662/1‑237 (MQ=255)       aTGAACTGCGCGAAGCAAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACAtggtgg                                                                                                                                                                                                                           <  1:35662/235‑1 (MQ=255)                gcgAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGGGAACATGGGGGGTTTAAAGAGatc                                                                                                                                                                                                              >  1:1006283/1‑239 (MQ=255)                gcgAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGTGCGCCACCCAGGGGGAACATGGGGGGTATAAAgagagc                                                                                                                                                                                                              >  1:1309798/1‑237 (MQ=255)                gcgAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGGGCCAGCGAGGGGGAACATGGTGGGTATAAAGAGatc                                                                                                                                                                                                              >  1:764380/1‑239 (MQ=255)                 cgAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGTAATCATGAGCGCCAGCCGGGGGGATAATGGTGGGTCTAAagcgctaa                                                                                                                                                                                                             >  1:1630632/1‑233 (MQ=255)                       aaaaGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCAAAAAGATCGTGATGACGAACGTACCGCCTTCCTCGAAGTCCGAGCCGGAACCGGGGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGcc                                                                                                                                                                                                        <  1:1373511/237‑1 (MQ=255)                              aaagGCGACCAACGGGAACAGAAATTACAGGTGCTGTTACTGCAAAAAGATCCGGATGCCGAACGTAACGCCTTCCTCGAAGGCCGAGCCGGACCGGGGGGCGACGAAGCGGCGCTGTTCGGGGGCGTTCTGTCCCGTATGTACGGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAg                                                                                                                                                                                                <  2:1004348/239‑1 (MQ=255)                              aaaaGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCGCCAGCGAGGGGGAAAATGGGGGGTATAAAAAGat                                                                                                                                                                                                               >  1:337017/1‑224 (MQ=255)                                aaGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGGCCCAGCGGGGGGGGAAACGGTGGTTATTAAAAGAACATCGCCCAAATTAACg                                                                                                                                                                                              >  2:1373511/1‑239 (MQ=255)                                    gagaAACTGGAACAGAAATTACAGGTTCTGTTACTGCCAAAAGTTCCTGATGACGAACGTACCGCCTTCCTCGAAGTCCGAGCGGGAACCGGCGGCGACGAGGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACGGCCGTTATGCCGAGGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTg                                                                                                                                                                                           <  2:1324275/238‑1 (MQ=255)                                    gagaAACTAGAACAGCAATTACAGTTTCTGTTACTGCAAAAAGATCCTGATGAAGACCGTAACGCCTTCCTCGAAGTCCGGGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCTGTTTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTg                                                                                                                                                                                           <  2:2199884/238‑1 (MQ=255)                                    gAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTg                                                                                                                                                                                           <  1:884947/238‑1 (MQ=255)                                         aCTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAAACATGAGGGCCAGCGAGGGGGAAAATGGGGGTTATAAAGAGATCATCGCCAAAATTAACGGGGAtcggg                                                                                                                                                                                     >  1:1908207/1‑235 (MQ=255)                                                       tACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTACCGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAGGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACAtgg                                                                                                                                                                                                                              <  1:478488/184‑1 (MQ=255)                                                                      tgaaaaaaGACCCTGATGACAAACAAACCCCCTTCCGCGAAGCCCGGCCCGGAACGGGGGGCCACGAAGCGGCGCTGTTCGCGGGCGATCTTTTCCTTCGTTACAGCCGTTATGCCGAGGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcggc                                                                                                                                                        <  2:764380/235‑1 (MQ=255)                                                                        ccAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAAACATGAGCGCCCGCGAGGGGGAACATGGGGGGTATTAAAAGATCAACGCCCAAAATAACGGGGAGTGGGGGTGTGGGGGGCTGGAATTTTAATCcggggg                                                                                                                                                      >  2:2270809/1‑239 (MQ=255)                                                                        caaaaaGACCCTGATGACGAACGTAACCCCTTCCCCGAAGCCCGGGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGGGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGt                                                                                                                                                                                                                                      <  2:91088/157‑1 (MQ=255)                                                                         aaaaaGATCCTGATGAAGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcgg                                                                                                                                                         <  1:1849865/234‑1 (MQ=255)                                                                            aaGACCCTGATGACAAACGTACCGCCTTCCTCGAGGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTg                                                                                                                                                                                           <  2:706149/198‑1 (MQ=255)                                                                               acccggatgaCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGTTCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGtt                                                                                                                                                                                                                         <  1:1470306/160‑1 (MQ=255)                                                                                  cTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCGCCAGCGGGGGGGAACAtggtg                                                                                                                                                                                                                            >  1:91088/1‑159 (MQ=255)                                                                                      tgaCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCGCCAGCGAGGGGGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGGGATGGTGGGTa                                                                                                                                                                                 >  1:706149/1‑198 (MQ=255)                                                                                         cGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGCAATCATGAGCGCCCGCGAGGGTGAACATGGGGGTTTTAAAGAGat                                                                                                                                                                                                               >  2:1470306/1‑165 (MQ=255)                                                                                            aCGTAGCGCTTTCCGCGCAGTAGGAGCCGGACCCGGCGGCGACGAAGCGGCGCTTTCCGCGGGGGATCTGTTCCGTATGTACGGCCTATATGCGGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGtt                                                                                                                                   <  2:1309798/238‑1 (MQ=255)                                                                                                       tCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACAtgg                                                                                                                                                                                                                              <  1:1435308/136‑1 (MQ=255)                                                                                                       tCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGGGAACAtgg                                                                                                                                                                                                                              >  2:1435308/1‑136 (MQ=255)                                                                                                            gAAGTCCGGGCCGGAACCGGGGGCGACGAAGCGGCGCTGTTCGGGGGCAATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑Ca                                                                                                                 <  1:2378664/239‑1 (MQ=255)                                                                                                                 ccGGGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTc                                                                                                            <  1:392544/239‑1 (MQ=255)                                                                                                                      cggcaccgggcggcGCGGAAGCGGGGTTGTCGGGGGACAATTTTTTCCGTAGGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTAt                                                                                                        <  1:115194/230‑1 (MQ=255)                                                                                                                       cGGAACGGGGGGCGACGAAGCGGCGCTGTTCGGGGGCGATCTGTTCCGTATGTACAGCGTTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTAtt                                                                                                       <  1:942206/238‑1 (MQ=255)                                                                                                                             cggcggCGACGAAGCGGCGGTTTTCGGGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACtt                                                                                                <  1:207936/239‑1 (MQ=255)                                                                                                                               gcggcgACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATTAAGGCCAGGGAGGGTGAACATGGTGGTTATaaa                                                                                                                                                                                                                    >  1:1503882/1‑122 (MQ=255)                                                                                                                                gggcgACGAAGGGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTCCAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACt                                                                                                 <  1:1236871/234‑1 (MQ=255)                                                                                                                                cggcgCCGAGGGGGCGTTGTTGGGGGGCGTTCTGTCCCTTATGTACGGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCt                                                                                              <  1:2270809/238‑1 (MQ=255)                                                                                                                                cggcgACGAAGCGGCGCTGTTCGGGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCt                                                                                              <  1:1646096/238‑1 (MQ=255)                                                                                                                                cggcgACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCGCCAGCGAGGGGGAACATGGTGGGTATAAAGAGATCATCGCCAAAATTAGCGGGGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGGAACG‑CAGGGGCGGATTCATACt                                                                                                 >  2:207936/1‑235 (MQ=255)                                                                                                                                     acgaAGCGGCGCTGTCCGCGGGCGTTCTTTTCCTTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATg                                                                                                                                                                                        <  2:510312/144‑1 (MQ=255)                                                                                                                                         aGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATg                                                                                                                                                                                        <  1:2012531/140‑1 (MQ=255)                                                                                                                                               gcTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGGGAACATGGGGGGTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGGGTGGATgg                                                                                                                                                                              >  1:510312/1‑144 (MQ=255)                                                                                                                                                 tGTTCGGGGGCGTGCTGTTCCGGTTGTACAGCCGTTATGCCGAGGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGt                                                                            <  2:934326/238‑1 (MQ=255)                                                                                                                                                   ttCGGGGGCGATCTGTTCCGTATGTACAGCCTTTAAGAGGAAGGCCGCCGCTGGCGGGTAGAAGTCATGGGCGCCAGCGAGGGTGAACAtgg                                                                                                                                                                                                                              <  2:1984237/92‑1 (MQ=255)                                                                                                                                                             aTCTGTTCCGTATGTCCAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGcc                                                                                                                                                                                                        <  1:2059445/104‑1 (MQ=255)                                                                                                                                                             aTCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGGGGGTAAACATGGGGGGTATaaa                                                                                                                                                                                                                    >  1:1984237/1‑92 (MQ=255)                                                                                                                                                             aTCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑tt                                                                                 <  1:1072916/221‑1 (MQ=255)                                                                                                                                                             aTCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCAATAGCGCCAGCGAGGGGGAACATGGGGGGTATAAAGAGATCATTGCCAAAAATAGGGGTGGTGGGGGGGATGGTCGTCTGAAATTTGAATCCGGCGGTCATCCGGGGGAACCGGTTCCTGCTACGGGATCGTCAGGG‑CGGATTTATACTTTTGCTTGTACCC‑TTTCGGGaactgcagaaat                                                                >  2:59748/1‑228 (MQ=255)                                                                                                                                                               ctgttcgtgaTGTACGGCCGTTGTGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑Gcc                                                            <  2:1006283/230‑1 (MQ=255)                                                                                                                                                                  ttcggtgggaaCAGCCTTTAGGCGGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGa                                                         <  2:1331449/229‑1 (MQ=255)                                                                                                                                                                  tcccGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGt                                                                                                             <  1:2391144/187‑1 (MQ=255)                                                                                                                                                                       tATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGGAAACTTTAGCGCCAGCGAGGGGGAACATGGTGGTTATAAAGAGATCATCGCCACAAATAgag                                                                                                                                                                                              >  2:2059445/1‑102 (MQ=255)                                                                                                                                                                          gTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCCGCGAGGGGGGACATGGTGGTTATAAAGAGATCCTCCCCAAAATTAGCGGGGATGGTGTGTATGGTCGTCTGAAATTTTAATCCGGCGGTCCTCGCGTGGCACGTGTTCCTTCTACGGAAACG‑CAGGGGCGGCTTTATACTTCTTCTTGTACCG‑GTGCCGTAAAGTCCGAACA‑Gcccgtagtctaa                                                  >  2:1806648/1‑228 (MQ=255)                                                                                                                                                                            aCAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGGGGTTATAAAGAGATCATCGCCCAAATTTGCg                                                                                                                                                                                              >  1:171862/1‑99 (MQ=255)                                                                                                                                                                            aCAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATTAGCGCCAGCGGGGGGGAACAAGGGGGGTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTTAATCCGGCGGTCATCGCGTGCAACGGGTTCCTGCTACGGGATCG‑CAGGGTCGTATTCATACTTCTGGTTTTCCCG‑CTGTGGTAATGCCAGAACT‑GCCTGACGCCGaaac                                                >  2:142866/1‑236 (MQ=255)                                                                                                                                                                                ccGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAAACAAGAGGGCCAGCGAGGGGGAAAATGGGGGTTATAAAGAGAACATCGCCCAAATTAGCGGGGATGGGGGGTATGGTCGGCTGAAATTTGAATCCGGCGGGCAACGCGGGGAACGGGTTTCCGCTACGGGAACC‑CAGGGGCCGATTCATACTTCTGCTTGGTCCGTTTGCGGAAAT‑CCAGAAATGGCCGGACGcgaaacgccgg                                            >  2:1709351/1‑229 (MQ=255)                                                                                                                                                                                   ttctGCCGAAGCCCGCCGCTGGCGGGCCGAAATCTCTAGCGCCCATGAAGGTGCACATGGGGGTTATCAACAGATAATCCCCAAAATTTGCGGGGATGGGTGGTATGGTCGTCTGAAATTTGAATCTCGCGGTCCTCCCGTGCAACGTGCTTCTGCTACGGGATCC‑CCCGGTCGCATTTCTACTTCTCCTTGTCTCC‑TTTCGGGAATGCCATACCA‑TCCTGACGCCGaaacgccctc                                          >  2:1671930/4‑229 (MQ=255)                                                                                                                                                                                   ttATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATg                                                                                                                                                                                        <  2:1369926/98‑1 (MQ=255)                                                                                                                                                                                   ttATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑Ca                                                                                                                 <  1:1910392/168‑1 (MQ=255)                                                                                                                                                                                   ttATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGGGATg                                                                                                                                                                                        >  1:1369926/1‑98 (MQ=255)                                                                                                                                                                                   ttATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCGCCAGCGAGGGGGAACATGGTGGGTATAAAGAGATCATCGCCAAAATTAGCGGGGATGGTGTGGATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGGGCAACGGGTTCCTGCTACGGAATCG‑CCGGGGCGGATTCATACTTCTGCTTGTACCG‑TTGCGGGAATGCCAGAACT‑GCCTGACCGAGAACTGCCgggaa                                        >  2:771475/1‑236 (MQ=255)                                                                                                                                                                                     aTGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGGGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGGGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCCGAACTGCCGGACATc                                      >  1:2174181/1‑239 (MQ=255)                                                                                                                                                                                        ccGAACCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAg                                                                                                                                                                                                <  2:1661478/85‑1 (MQ=255)                                                                                                                                                                                                  ccgcTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGGGAACATGGGGGGTATAAAGAGATCATCGCCCAAATTAGCGGGGATggg                                                                                                                                                                                      >  1:1661478/1‑84 (MQ=255)                                                                                                                                                                                                  ccgcTGGCGGGGAGAAATCATGAGCGCCAGCGAGGGGGAACATGGGGGGTATAAAGAGATCATCGCCCAAATTAGCGGGGATGGGGGGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTTTGCTTGTACCG‑TTGCGGGAATGCCAGAACT‑GCCTGACGCAGAAATGCCGGGACTCCACCCAGCAGAtt                         >  2:2262501/1‑239 (MQ=255)                                                                                                                                                                                                         cGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCa                   <  1:142866/238‑1 (MQ=255)                                                                                                                                                                                                             tAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGa               <  1:2301505/238‑1 (MQ=255)                                                                                                                                                                                                             tAGAAAACATGAGCGCCAGCGAGGGGGGACATGGGGGTTTTTTAGAGATTATCGCCAAAATTAGCGGGGGTGGGGTGTATGGTTGTCTGAAATTTTAATCCGGCGGTCCTCGCGTGGAACGGGTTCCTGCTACGGAATCG‑CAGGGTCGTATTTATACTTTTGCTTTTACCC‑TTTCGGTAATGCCCGAAAT‑TCCTTGCGCAGAACTGCCGGGGATCAACCCACCAGAtttcagccattac              >  2:159150/1‑229 (MQ=255)                                                                                                                                                                                                                  aTCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGAt              >  1:1598459/1‑234 (MQ=255)                                                                                                                                                                                                                     aTGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑Ca                                                                                                                 <  2:1605564/134‑1 (MQ=255)                                                                                                                                                                                                                     aTGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑Ca                                                                                                                 >  1:1605564/1‑134 (MQ=255)                                                                                                                                                                                                                      tGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGt                                                                                                             >  2:186601/1‑137 (MQ=255)                                                                                                                                                                                                                      tGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGt                                                                                                             <  1:186601/137‑1 (MQ=255)                                                                                                                                                                                                                          cgcCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCg  >  1:970873/1‑238 (MQ=255)                                                                                                                                                                                                                            ccAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATg                                                                                                                                                                                        >  2:695617/1‑57 (MQ=255)                                                                                                                                                                                                                            ccAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATg                                                                                                                                                                                        <  1:695617/57‑1 (MQ=255)                                                                                                                                                                                                                            ccAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTa                       <  2:2175041/215‑1 (MQ=255)                                                                                                                                                                                                                            ccAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTa                       >  1:2175041/1‑215 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     CGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG‑CAGGGTCGTATTCATACTTCTGCTTGTACCG‑TTGCGGTAATGCCAGAACT‑GCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCG  >  NC_000913/1265244‑1265698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑