New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3220246	118 (0.980)	4 (0.030)	4/548	12.3	3.1%	coding (753/1380 nt)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
	?	NC_000913	= 3220398	136 (1.170)					coding (905/1380 nt)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3219950‑3220246 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgccgAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGGTTTTTTTGCACTCGTTAAGAGCCGTGCGCA  <  NC_000913/3220398‑3220146                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        gCCGTAGAAGCAGCGCTGAATCTGGCGAACGCTTACCAGTCACCGCGCGGAAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGTCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGC                                                                                                                                                                                                                                                                       <  2:811267/288‑1     TTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCC                                                                                                                                                                                                                                                                   <  1:969137/289‑1       GAAGCAGCGCTGAAGCTGCAGAAAGCTTACCAGTCACCGCGCGGCGAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGC                                                                                                                                                                                                                                                                 <  1:197942/289‑1       GAAGCAGCGCCGAAGCTGGCGAAAGCTTACCAGTCCCCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAGATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGC                                                                                                                                                                                                                                                                 <  2:161860/289‑1               GCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGCCAATATCGAAGCCATGCGCACGGCTCTTCACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCCCGAACCGATTCCGGGTGAAGGTGGCGGAATTCTGCCGCCcgcgggccat                                                                                                                                                                                                                                                         >  1:993681/1‑279                                                                                                                                                                                                                   AACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGGGAAGGTGGCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCCTCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGCGAGATAACCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATaa                                                     >  1:293714/1‑287                                                                                                                                                                                                                                     GGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGAGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCTAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGGGAGATAGCCCGGCGGCGGCAGAATTCGGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCCGCCACGTCATCAC                                   >  1:633340/1‑289                                                                                                                                                                                                                                                 GCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGACCACCGTAGCCACATCATCCCCGGCTTTTTTG                        >  1:380602/1‑288                                                                                                                                                                                                                                                                      ATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGACCACCGCAGCCACATCATCACCGGTTTTTTTGCACTCGTTAAGAGCCGTGCGCA  >  1:168302/1‑289                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        TCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3219950‑3220246 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccgccgccgAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCAGAATTACGCCACCTTCACCCTGAATCGGTTCGAGGATCACCGCAGCCACATCATCACCGGTTTTTTTGCACTCGTTAAGAGCCGTGCGCA  <  NC_000913/3220398‑3220146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.