Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,429	(T)5→4	intergenic (‑288/‑269)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,425	0	T	.	100.0%	159.4 / NA	37	intergenic (‑284/‑273)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/18);  total (19/18)

ATCAGACAAATAACGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTC  >  NC_000913/1755213‑1755630                                                                                                                                                                                                                     |                                                                                                                                                                                                              aTCAGACAAATAACGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCa                                                                                                                                                                                    <  2:542160/239‑1 (MQ=255)                                  gTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATgg                                                                                                                                                   >  1:656196/1‑239 (MQ=255)                                    ggCAATTGAGCGATGATATATTTATAAACCGGATGACCTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTACCCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt                                                                                                                                                     <  2:656196/234‑1 (MQ=255)                                                          tataCGCCGGATGAACTTTCACTTATCCTCACACTGACCACTTCGGCCCCAGACGTTGAGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGAGCAAAtt                                                                                                                                                                <  2:212482/202‑1 (MQ=255)                                                           ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCa                                                                                                                                                                                    >  1:91320/1‑181 (MQ=255)                                                           ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCa                                                                                                                                                                                    <  2:91320/181‑1 (MQ=255)                                                           ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGta                                                                                                                                                                               >  1:158636/1‑186 (MQ=255)                                                           ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGta                                                                                                                                                                               <  2:158636/186‑1 (MQ=255)                                                           ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGttt                                                                                                                                                <  1:424137/217‑1 (MQ=255)                                                           ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGttt                                                                                                                                                >  2:424137/1‑217 (MQ=255)                                                                   gATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGttt                                                                                                                                                <  1:149880/209‑1 (MQ=255)                                                                   gATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGttt                                                                                                                                                >  2:149880/1‑209 (MQ=255)                                                                   gATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACt                                                                                                                  >  1:527160/1‑239 (MQ=255)                                                                    aTGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGCTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGaa                                                                                                                                                      >  1:212482/1‑202 (MQ=255)                                                                         cTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCaaaaa                                                                                                            <  2:639120/239‑1 (MQ=255)                                                                                             gACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCa                                                                                                                                                                                    >  2:8342/1‑147 (MQ=255)                                                                                             gACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCa                                                                                                                                                                                    <  1:8342/147‑1 (MQ=255)                                                                                              aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTga                                                                                       >  2:256882/1‑239 (MQ=255)                                                                                                        cACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGa                                                                                                                                    <  2:200489/184‑1 (MQ=255)                                                                                                        cACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGa                                                                                                                                    >  1:200489/1‑184 (MQ=255)                                                                                                          ccAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGcaca                                                                           <  1:214353/239‑1 (MQ=255)                                                                                                                  tGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACAtt                                                                       >  2:307357/1‑235 (MQ=255)                                                                                                                                   ttttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGAt                                                   <  1:307357/238‑1 (MQ=255)                                                                                                                                     ttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGAt                                                   <  1:633677/236‑1 (MQ=255)                                                                                                                                     ttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGAt                                                   >  2:633677/1‑236 (MQ=255)                                                                                                                                         gTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAcc                                             <  1:256882/238‑1 (MQ=255)                                                                                                                                                 ttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACt                                                                                                                  <  1:90645/161‑1 (MQ=255)                                                                                                                                                 ttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACt                                                                                                                  >  2:90645/1‑161 (MQ=255)                                                                                                                                                        gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTTAGCACTTTGGACTGTAGAACCCAACGACTCAAAAACAGGCACTAACGTTGGGCTGAGACACTAGCACACATTCCCCTGTACGCTTTCTCGATGTCACCCATCCTTAGAGCGAg                              >  2:623009/1‑238 (MQ=255)                                                                                                                                                        gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGa                                                    >  1:293596/1‑216 (MQ=255)                                                                                                                                                        gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGa                                                    <  2:293596/216‑1 (MQ=255)                                                                                                                                                             ttAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcacc                         >  1:496901/1‑238 (MQ=255)                                                                                                                                                                                      tCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGc                                                                              >  2:567814/1‑160 (MQ=255)                                                                                                                                                                                      tCTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGc                                                                              <  1:567814/160‑1 (MQ=255)                                                                                                                                                                                       cTAACAAGTTGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTc  >  1:387073/1‑235 (MQ=255)                                                                                                                                                                                                tGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACt                                                                                                                                         >  1:405697/1‑91 (MQ=255)                                                                                                                                                                                                tGTATATTTTTTGAAACGCTG‑TTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACt                                                                                                                                         <  2:405697/91‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                              ATCAGACAAATAACGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTC  >  NC_000913/1755213‑1755630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑