| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,628,644 | A→T | C99S (TGT→AGT) | yfgF ← | cyclic‑di‑GMP phosphodiesterase, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,628,644 | 0 | A | T | 100.0% | 65.9 / NA | 18 | C99S (TGT→AGT) | yfgF | cyclic‑di‑GMP phosphodiesterase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (10/8); total (10/8) | |||||||||||
AGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTATTAAT > NC_000913/2628425‑2628845 | aGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAACCTGTAACCGGCCCAACTGGGaatga > 2:89110/1‑228 (MQ=255) tAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCCGGGAGTAAAGACCCTGTAACCGCACCAACTTAGAATGGTAATGATAATCAAATTaca > 2:405471/1‑237 (MQ=255) aTGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCa > 1:44772/1‑238 (MQ=255) aTTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGc > 1:633990/1‑239 (MQ=255) ccaaaaatggcagaaAACGGAAAAAGTATAAAAAACAGCGTTGCAGAAAACACAATCTGCAAGAATATACGCTGGAAATTTAAACGGGTATCCCCATGTAAAACGTTTTTACGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGttt < 2:493140/181‑1 (MQ=255) aCAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACACAAATCCCTAAGGCGATc > 1:56117/1‑237 (MQ=255) cagcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAcc > 1:493140/1‑190 (MQ=255) aaaTTAGCAAAAACGGCGTTGCAGAAACCAAATCCTGCAAGAATATACGCTGGAAAATAAACCGGGTATCCCAATGAAAACCGTTTTTACGCGGGGAAAAAAAGCCCCTATAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTg < 2:44772/239‑1 (MQ=255) tATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaa > 1:733737/1‑239 (MQ=255) aaCGGCTTTGAAGGAACCCAAATCGGCAAGAATACAGGATGGAAAATTAACCGGTTACCACAATGTAAACCGTTGTTCCGCCGGGAAGAAAAGCCCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAAcatcat < 2:56117/239‑1 (MQ=255) aaaCACAAGCTGACAAAATATACGCTAGGAAATTACGCGAGTATCACCAGGTAAATGGTTGTACCGCGGGGGAATAAACACCTTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGatcat < 2:633990/237‑1 (MQ=255) tataCGCTGGGAAATTAACCGGGTATGACCATGTGAACCGTTGTAACGCGGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATaaa < 1:20098/239‑1 (MQ=255) gTATCACCATGTAAAACGTTGTTACGCCGGGAAGAAAAGCCCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaaa < 2:38583/179‑1 (MQ=255) tctccAGGTGAATCGTGTTAACGCCGGGGAGGAAAGCCCCGTTATCCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg < 2:504498/110‑1 (MQ=255) gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATg > 1:38583/1‑179 (MQ=255) gAAACGTTGTTACGCCGGGAAGTAAAGCCCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGa < 1:405471/238‑1 (MQ=255) aaaCGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat > 1:504498/1‑113 (MQ=255) ttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCCGCAAAGGATTAAt > 2:313213/1‑239 (MQ=255) | AGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTATTAAT > NC_000913/2628425‑2628845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |