Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,181,174	G→A	G252S (GGC→AGC)	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,174	0	G	A	100.0%	200.6 / NA	50	G252S (GGC→AGC)	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/22);  total (28/22)

GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCG  >  NC_000913/3180961‑3181408                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           gACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTg                                                                                                                                                                                                                   <  1:719197/239‑1 (MQ=255)                                aTTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACt                                                                                                                                                                                        <  1:594317/235‑1 (MQ=255)                                         aCTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                                                                                                                                                                           >  2:729078/1‑207 (MQ=255)                                         aCTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                                                                                                                                                                           <  1:729078/207‑1 (MQ=255)                                             cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACg                                                                                                                                                                                                                                     <  2:319356/177‑1 (MQ=255)                                             cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACg                                                                                                                                                                                                                                     >  1:319356/1‑177 (MQ=255)                                                       gCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTccc                                                                                                                                                             >  1:472240/1‑239 (MQ=255)                                                                   aCTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGa                                                                                                                                                  >  1:652809/1‑238 (MQ=255)                                                                       aGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACt                                                                                                                                                                                        >  1:296290/1‑196 (MQ=255)                                                                       aGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACt                                                                                                                                                                                        <  2:296290/196‑1 (MQ=255)                                                                          tCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACctgctg                                                                                                                                          <  2:652809/239‑1 (MQ=255)                                                                                 cATCGATGATATCGGGTTAGGTGAAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCt                                                                                                                                    >  1:164782/1‑239 (MQ=255)                                                                                   tCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGa                                                                                                                                                                                                                 >  1:655279/1‑159 (MQ=255)                                                                                   tCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGa                                                                                                                                                                                                                 <  2:655279/159‑1 (MQ=255)                                                                                    cGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgc                                                                                                                                                                                                                                    >  2:462562/1‑139 (MQ=255)                                                                                    cGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgc                                                                                                                                                                                                                                    <  1:462562/139‑1 (MQ=255)                                                                                       tgatATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTg                                                                                                                             <  1:618398/239‑1 (MQ=255)                                                                                         atatCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCg                                                                                                                           >  1:16615/1‑239 (MQ=255)                                                                                                gTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAgatg                                                                                                                    >  2:531288/1‑239 (MQ=255)                                                                                                     gTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTa                                                                                                                                                                              >  1:146148/1‑176 (MQ=255)                                                                                                     gTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTa                                                                                                                                                                              <  2:146148/176‑1 (MQ=255)                                                                                                         aaaaaGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGaa                                                                                                                                                                                                                <  1:643831/139‑1 (MQ=255)                                                                                                         aaaaaGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGaa                                                                                                                                                                                                                >  2:643831/1‑139 (MQ=255)                                                                                                           aaaGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCGACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTCATTTTTCGGAAGATGAGACTCCGCa                                                                                                          >  2:329427/1‑239 (MQ=255)                                                                                                           aaaGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCa                                                                                                          >  1:729619/1‑239 (MQ=255)                                                                                                                 cAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                                                                                                                                                                           <  2:68540/136‑1 (MQ=255)                                                                                                                 cAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGGTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                                                                                                                                                                           >  1:68540/1‑136 (MQ=255)                                                                                                                     tCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATgg                                                                                                     <  1:531288/234‑1 (MQ=255)                                                                                                                       aCGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAata                                                                                              <  1:331037/239‑1 (MQ=255)                                                                                                                              tACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGtt                                                                                       >  1:598471/1‑239 (MQ=255)                                                                                                                                    aTCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTa                                                                                      <  2:729619/234‑1 (MQ=255)                                                                                                                                     tCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                                                                                                                                                                           <  2:303966/116‑1 (MQ=255)                                                                                                                                     tCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                                                                                                                                                                           >  1:303966/1‑116 (MQ=255)                                                                                                                                     tCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGa                                                                                >  2:576380/1‑239 (MQ=255)                                                                                                                                                 cAACCTTGTCAAACGTGATCCGTTGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTg                                                                    <  2:16615/239‑1 (MQ=255)                                                                                                                                                 cAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTg                                                                    <  1:576380/239‑1 (MQ=255)                                                                                                                                                        ttCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAgg                                                                                                                                                                                            >  1:710180/1‑112 (MQ=255)                                                                                                                                                        ttCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAgg                                                                                                                                                                                            <  2:710180/112‑1 (MQ=255)                                                                                                                                                        ttCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcgc                                                             >  1:63536/1‑239 (MQ=255)                                                                                                                                                                gTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgtgt                                                     <  2:164782/239‑1 (MQ=255)                                                                                                                                                                  aTCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCg                                                   >  2:208514/1‑239 (MQ=255)                                                                                                                                                                       tGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGACAAATATGTGGTTAAACCGATCTTCTCCCTTGAAGGCGCAAACGTGTCGATCAt                                              >  2:489461/1‑239 (MQ=255)                                                                                                                                                                       tGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtcttc                                                                           >  1:75906/1‑210 (MQ=255)                                                                                                                                                                       tGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtcttc                                                                           <  2:75906/210‑1 (MQ=255)                                                                                                                                                                       tGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCAt                                              >  1:172373/1‑239 (MQ=255)                                                                                                                                                                           aaTTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTggg                                          >  1:440339/1‑237 (MQ=255)                                                                                                                                                                                    tGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                                 <  2:172373/239‑1 (MQ=255)                                                                                                                                                                                     gCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                                 >  1:602609/1‑238 (MQ=255)                                                                                                                                                                                                tctcAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagca                     >  1:616031/1‑239 (MQ=255)                                                                                                                                                                                                                   tGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCg  >  1:718801/1‑239 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCG  >  NC_000913/3180961‑3181408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑