Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,881	A→T	E546V (GAA→GTA)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,881	0	A	T	100.0%	193.6 / NA	50	E546V (GAA→GTA)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/28);  total (22/28)

ACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGA  >  NC_000913/4182651‑4183115                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           aCGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCga                                                                                                                                                                                                                                     <  1:482423/238‑1 (MQ=255)              gCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACAcc                                                                                                                                                                                                                           <  2:311256/235‑1 (MQ=255)              gCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACAcc                                                                                                                                                                                                                           >  1:311256/1‑235 (MQ=255)              gCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                                                                                                                                                                        >  1:545487/1‑238 (MQ=255)              ccGGTGAAAGGGCGTCTGTCTGTGGGCGATCTGGATTCCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                                                                                                                                                                        <  2:114130/237‑1 (MQ=255)                   gAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACt                                                                                                                                                                                                                  >  1:449642/1‑239 (MQ=255)                          cGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACt                                                                                                                                                                                                                  >  2:101208/1‑232 (MQ=255)                                  tcggggCGATGTGGA‑ATCCTGAATCCCACAGGATAGGATCAACGCCAGGCCAATTTCCGCAGCAGTGAAAGAGTTCTTCGTTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTc                                                                                                                                                                                                   <  2:482488/236‑1 (MQ=255)                                          aTCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                                                                                                                                                                        >  2:215832/1‑210 (MQ=255)                                          aTCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                                                                                                                                                                        <  1:215832/210‑1 (MQ=255)                                            tgggATACCCTT‑ATGCCAAAGGATATGATCAACGCAAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAc                                                                                                                                                                                         <  2:545487/237‑1 (MQ=255)                                                    ccTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                                                                                                                                                                        >  1:161491/1‑200 (MQ=255)                                                    ccTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                                                                                                                                                                        <  2:161491/200‑1 (MQ=255)                                                    ccTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg                                                                                                                                                                                 >  1:641911/1‑239 (MQ=255)                                                       gttttgcccaGGGTTTGATCAACGCAAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGt                                                                                                                                                                               <  2:155260/231‑1 (MQ=255)                                                          tGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                                                                                                                                                             <  1:101208/238‑1 (MQ=255)                                                                   atatGAACAACGACAAGCCGATTTCCGAAGCAGTGAAAGAATTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                                                                     <  1:204588/237‑1 (MQ=255)                                                                    tatGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                                                                                                   <  2:641911/238‑1 (MQ=255)                                                                                       aTTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCga                                                                                                                                                                                                                                     <  2:61012/153‑1 (MQ=255)                                                                                       aTTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCga                                                                                                                                                                                                                                     >  1:61012/1‑153 (MQ=255)                                                                                                  agTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                                                                        <  1:594387/235‑1 (MQ=255)                                                                                                                         gccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag                                                                                                             >  1:418143/1‑239 (MQ=255)                                                                                                                                                   aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACt                                                                                                                                                                                                                      >  2:18996/1‑108 (MQ=255)                                                                                                                                                   aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACt                                                                                                                                                                                                                      <  1:18996/108‑1 (MQ=255)                                                                                                                                                    cccacCCGATGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGTTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg                                                                                       <  2:618318/231‑1 (MQ=255)                                                                                                                                                    acagcCCGCTGTCTGAGATTACGCACAAACGTCGAACCTCCGCCCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                                                                     <  2:343921/236‑1 (MQ=255)                                                                                                                                                        cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                                                                                                                                                                                        >  2:216449/1‑117 (MQ=255)                                                                                                                                                        cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                                                                                                                                                                                        <  1:216449/117‑1 (MQ=255)                                                                                                                                                          cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActct                                                                                                                                                       <  2:185105/164‑1 (MQ=255)                                                                                                                                                          cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActct                                                                                                                                                       >  1:185105/1‑164 (MQ=255)                                                                                                                                                             tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                                                                                 <  2:385635/151‑1 (MQ=255)                                                                                                                                                             tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                                                                                 >  1:385635/1‑151 (MQ=255)                                                                                                                                                                         cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACt                                                                                                                                                                                                                      >  1:593324/1‑86 (MQ=255)                                                                                                                                                                         cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACt                                                                                                                                                                                                                      <  2:593324/86‑1 (MQ=255)                                                                                                                                                                         cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGt                                                                                                                                                                                                             <  1:132571/95‑1 (MQ=255)                                                                                                                                                                         cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGt                                                                                                                                                                                                             >  2:132571/1‑95 (MQ=255)                                                                                                                                                                                   tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa                                                                                                <  1:719028/194‑1 (MQ=255)                                                                                                                                                                                   tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa                                                                                                >  2:719028/1‑194 (MQ=255)                                                                                                                                                                                                  cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGCGACTCCGTATCGTAAAGTGACAGACGGAGTTGTAACTGACGGAATTAACTACCTGTCTGCTATCGAAGAAGGCa                                    >  2:560878/1‑239 (MQ=255)                                                                                                                                                                                                      ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGAc                                                                                                                                                                                                                                  <  1:535283/45‑1 (MQ=255)                                                                                                                                                                                                      ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGAc                                                                                                                                                                                                                                  >  2:535283/1‑45 (MQ=255)                                                                                                                                                                                                      ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACggagt                                                                                                                    <  2:189221/155‑4 (MQ=255)                                                                                                                                                                                                      ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACggagt                                                                                                                    >  1:189221/1‑152 (MQ=255)                                                                                                                                                                                                      ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGGGTTGTAACTGACGAAATTTACTACCTGCTTGCTATCGCAGAAGGCAACTa                                >  2:433479/1‑239 (MQ=255)                                                                                                                                                                                                             gTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCgaagaa                                        <  2:294708/224‑1 (MQ=255)                                                                                                                                                                                                             gTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCgaagaa                                        >  1:294708/1‑224 (MQ=255)                                                                                                                                                                                                             gTCTGACCCGTGAACGTGCAGGCTCCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACg                                                                                    <  2:511076/180‑1 (MQ=255)                                                                                                                                                                                                             gTCTGACCCGTGAACGTGCAGGCTCCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACg                                                                                    >  1:511076/1‑180 (MQ=255)                                                                                                                                                                                                                      gTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCg                <  1:433479/239‑1 (MQ=255)                                                                                                                                                                                                                                     tCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGa  <  1:439884/238‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           ACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG‑ATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGA  >  NC_000913/4182651‑4183115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑