Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,881 A→T E546V (GAA→GTA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,8810AT99.1% 747.0 / ‑1.7 219E546V (GAA→GTA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (115/100);  total (116/103)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 219 total aligned reads displayed.

TTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGT  >  NC_000913/4182785‑4182976
                                                                                                |                                                                                               
ttATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAg                                                                                               <  1:1823302/99‑1 (MQ=255)
  aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGtt                                                                                             <  2:2920558/99‑1 (MQ=255)
    ggACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCg                                                                                           <  1:1938750/99‑1 (MQ=255)
     gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCga                                                                                          >  1:5415459/1‑99 (MQ=255)
     gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCga                                                                                          <  2:3021942/99‑1 (MQ=255)
      aCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCgag                                                                                         <  1:4514118/99‑1 (MQ=255)
      aCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCgag                                                                                         >  2:3735480/1‑99 (MQ=255)
       ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCgaga                                                                                        >  2:554780/1‑99 (MQ=255)
       ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCgaga                                                                                        >  1:3585384/1‑99 (MQ=255)
        cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGAc                                                                                       <  1:1229647/99‑1 (MQ=255)
        cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGAc                                                                                       >  1:4571503/1‑99 (MQ=255)
        cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGAc                                                                                       >  1:5573188/1‑99 (MQ=255)
        cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGAc                                                                                       >  2:1314050/1‑99 (MQ=255)
          gAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCCCTCGGCCCAGGCGTTCTGACCCGTGAACGTGCAGGCTTCGCAGTTCGAGACGt                                                                                     >  2:2052236/1‑99 (MQ=255)
          gAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGCACGTGCAGGCTTCGTAGTTCGAGACGt                                                                                     >  2:423653/1‑99 (MQ=255)
          gAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGt                                                                                     >  1:1127805/1‑99 (MQ=255)
          gAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGt                                                                                     >  2:3833548/1‑99 (MQ=255)
           aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTa                                                                                    <  2:657411/99‑1 (MQ=255)
           aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTa                                                                                    <  1:4115748/99‑1 (MQ=255)
             caacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTaca                                                                                  >  1:1753919/1‑99 (MQ=255)
              aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTacac                                                                                 >  1:2970544/1‑99 (MQ=255)
              aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTacac                                                                                 <  1:3297939/99‑1 (MQ=255)
              aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTacac                                                                                 <  2:623706/99‑1 (MQ=255)
                cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACAccc                                                                               <  1:4685296/99‑1 (MQ=255)
                cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACCTACAccc                                                                               >  2:2850175/1‑99 (MQ=255)
                 ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTCCACCCg                                                                              >  2:2262361/1‑99 (MQ=255)
                  cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                             <  2:4855651/99‑1 (MQ=255)
                  cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                             <  1:2141130/99‑1 (MQ=255)
                  cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGa                                                                             >  1:3099419/1‑99 (MQ=255)
                   gCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGAc                                                                            >  1:462719/1‑99 (MQ=255)
                       tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGAGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGTCTCAc                                                                        >  1:2277630/1‑99 (MQ=255)
                        cTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACt                                                                       <  1:3381464/99‑1 (MQ=255)
                         tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTa                                                                      >  2:4958287/1‑99 (MQ=255)
                           agaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACg                                                                    <  1:378234/99‑1 (MQ=255)
                              ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTc                                                                 >  2:4323906/1‑99 (MQ=255)
                                  gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                                             >  1:3646027/1‑99 (MQ=255)
                                  gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                                             >  2:2671929/1‑99 (MQ=255)
                                  gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                                             >  1:3314388/1‑99 (MQ=255)
                                  gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                                             >  1:4752886/1‑99 (MQ=255)
                                   cacaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTa                                                            >  2:894998/1‑99 (MQ=255)
                                    acaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTAt                                                           <  1:180383/99‑1 (MQ=255)
                                      aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGt                                                         <  2:2106935/99‑1 (MQ=255)
                                      aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGt                                                         >  1:2886256/1‑99 (MQ=255)
                                       aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTc                                                        <  1:13925/99‑1 (MQ=255)
                                       aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTc                                                        <  1:161625/99‑1 (MQ=255)
                                          gtcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCaa                                                     <  1:2176576/99‑1 (MQ=255)
                                           tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAAt                                                    >  2:2952235/1‑99 (MQ=255)
                                           tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAAt                                                    <  1:2845866/99‑1 (MQ=255)
                                              tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                                                 >  2:3027664/1‑99 (MQ=255)
                                              tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                                                 >  1:943856/1‑99 (MQ=255)
                                                tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGCGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaaa                                               >  1:4176269/1‑99 (MQ=255)
                                                 ctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCGCTACGGTCGCGTATGTCCAATCGAAAc                                              <  2:1860299/99‑1 (MQ=255)
                                                  tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAcc                                             >  1:1490077/1‑99 (MQ=255)
                                                   ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAccc                                            <  2:3608255/99‑1 (MQ=255)
                                                   ccGCACTCCGCCCCGGCGGCCTGACCCGTGAACGTGCCGGCCCCGTAGTTCGACCCGTACACCCCACTCACTACGGCCGCGTCTCTCCAATCCAAAccc                                            >  2:1891083/1‑99 (MQ=255)
                                                    cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAcccc                                           >  2:4730735/1‑99 (MQ=255)
                                                    cGCACTCGGCCCAGCCGGTCTGCCCCGTGAACCTGCCCGCTTCGTCGTTCGACCCCTACTCCCTACTCACTATAACCCCGCATGTCACATCGCAAcccc                                           >  2:4347497/1‑99 (MQ=255)
                                                    cGCACTCAGCCCAGGCGGTCTGACCCGGGAACGTGCAGGCTTCGTAGTTCGAGCCGTACACCCGACTCACTACGGTCGCGTATGTCCATTCGAAAccct                                           <  2:5125381/99‑2 (MQ=255)
                                                     gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCt                                          >  1:3732065/1‑99 (MQ=255)
                                                     gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCt                                          >  1:3976859/1‑99 (MQ=255)
                                                        cTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa                                       >  1:5313477/1‑99 (MQ=255)
                                                         tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg                                      >  1:2605075/1‑99 (MQ=255)
                                                         tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg                                      <  1:4367108/99‑1 (MQ=255)
                                                             cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                  >  1:5539128/1‑99 (MQ=255)
                                                             cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                  >  1:4621837/1‑99 (MQ=255)
                                                              ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg                                 >  1:5139770/1‑99 (MQ=255)
                                                              ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg                                 <  1:4657685/99‑1 (MQ=255)
                                                                 ggCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc                              >  1:3943862/1‑99 (MQ=255)
                                                                   cGGTCTGACCCGTGCACGAGCAGGCGTCGGAGTTCGAGACGAACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAcct                            <  2:2489153/99‑3 (MQ=255)
                                                                    ggccTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc                           <  1:796439/96‑1 (MQ=255)
                                                                    ggTCTGACCCGTGAACGTGCCGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc                           <  2:2700257/99‑1 (MQ=255)
                                                                    ggTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc                           >  1:1288704/1‑99 (MQ=255)
                                                                     gTCTGACCCGTGACCGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACGCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                          <  1:5081153/99‑1 (MQ=255)
                                                                     gTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                          <  2:1562799/99‑1 (MQ=255)
                                                                     gTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                          >  1:4923453/1‑99 (MQ=255)
                                                                     gTCTCACCCGAGAACGTGAAGGCTTCGTAGTTCGAGACGGACACCCGACCCACTACGGTCGCGTATGTACAATCGAAACGCCTGATGGTCCGAACATCg                          <  2:2334451/99‑1 (MQ=255)
                                                                      tCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCgg                         >  2:3464819/1‑99 (MQ=255)
                                                                      tCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCgg                         >  1:4166139/1‑99 (MQ=255)
                                                                       cTGACACGTGATCGTGCAGGAATCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                        <  2:3320222/99‑1 (MQ=255)
                                                                        tGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGGCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc                       <  1:108452/99‑1 (MQ=255)
                                                                        tGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc                       <  2:1644761/99‑1 (MQ=255)
                                                                         gACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                      <  1:4298406/99‑1 (MQ=255)
                                                                          aCCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTCCGGGCGCGTATGTCAAATGGAAACCCCTGAAGGTCCGAACATCGGTCTg                     >  2:4021157/1‑99 (MQ=255)
                                                                            ccGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt                   <  1:3203123/99‑1 (MQ=255)
                                                                            ccGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt                   >  1:4961893/1‑99 (MQ=255)
                                                                            ccGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt                   <  2:1525505/99‑1 (MQ=255)
                                                                             cGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATc                  <  1:2208825/99‑1 (MQ=255)
                                                                              gTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa                 >  2:2609916/1‑99 (MQ=255)
                                                                               tGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTGCGAACATCGGTCTGGTCaa                >  2:5435482/1‑99 (MQ=255)
                                                                                gAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAc               <  2:2767520/99‑1 (MQ=255)
                                                                                   cGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActct            <  2:2560947/99‑1 (MQ=255)
                                                                                    gTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc           <  2:389173/99‑1 (MQ=255)
                                                                                      gCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg         >  1:1541401/1‑99 (MQ=255)
                                                                                      gCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg         >  1:2706479/1‑99 (MQ=255)
                                                                                         ggCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc      <  1:2647065/99‑1 (MQ=255)
                                                                                         ggCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc      <  2:3624289/99‑1 (MQ=255)
                                                                                           cTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt    <  1:2798671/99‑1 (MQ=255)
                                                                                           cTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt    <  2:118834/99‑1 (MQ=255)
                                                                                            ttCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtg   >  2:4833855/1‑99 (MQ=255)
                                                                                             tCGTAGTTCGAGAAGTACACCCGCCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGACCATCGGTCTGATCAACTCTCTGTCCgtgt  <  2:1396061/99‑1 (MQ=255)
                                                                                                |                                                                                               
TTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGT  >  NC_000913/4182785‑4182976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑