Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,755,429 (T)5→4 intergenic (‑288/‑269) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,755,4250T.95.4% 739.5 / 28.5 175intergenic (‑284/‑273)ydhZ/pykFuncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base . (67/99);  total (73/102)
Fisher's exact test for biased strand distribution p-value = 7.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

CGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTC  >  NC_000913/1755328‑1755519
                                                                                                 |                                                                                              
cgcAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTTTCTAACAAGTTGTATATTTTTTGAAACGCtgtt                                                                                               >  2:2985174/1‑99 (MQ=255)
  cAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgtttt                                                                                             <  1:2419232/99‑1 (MQ=255)
   aaaCAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATAGTCTAACAAGTTGTATATTTTTTGAAACGCtgttttg                                                                                            >  2:3881999/1‑98 (MQ=255)
     aCAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgttttgtt                                                                                          <  2:826986/99‑4 (MQ=255)
         tGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTcc                                                                                     >  1:3847126/1‑99 (MQ=255)
         tGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTcc                                                                                     >  1:3674440/1‑99 (MQ=255)
         tGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTcc                                                                                     >  2:2770563/1‑99 (MQ=255)
         tGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTAGTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTc                                                                                      <  2:3646870/99‑1 (MQ=255)
            aGTTTTTGCGTAACCTTTTTCCTGAAACGTCAAATCTTTAATAACAATTTATTGTCTAACAAGTTGTATATTATTTGAAACGCTCTTTTGTTTTCCttt                                                                                  <  2:260502/99‑1 (MQ=255)
             gTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCtttt                                                                                 >  1:1060797/1‑99 (MQ=255)
             gTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATCGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCtttt                                                                                 <  2:114019/99‑1 (MQ=255)
              tttttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTg                                                                                <  1:3924371/99‑1 (MQ=255)
                tttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGa                                                                              <  1:84039/99‑1 (MQ=255)
                tttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGa                                                                              <  1:2756487/99‑1 (MQ=255)
                     gTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAAt                                                                         >  2:3974184/1‑99 (MQ=255)
                     gTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAAt                                                                         <  2:3102462/99‑1 (MQ=255)
                     gTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAAt                                                                         >  1:1275705/1‑99 (MQ=255)
                        aCCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTc                                                                      >  2:2012430/1‑99 (MQ=255)
                           ttttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGc                                                                   <  1:3936074/99‑1 (MQ=255)
                           ttttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGc                                                                   <  2:3907902/99‑1 (MQ=255)
                           ttttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGc                                                                   <  1:1726674/99‑1 (MQ=255)
                           ttttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGc                                                                   <  1:1396250/99‑1 (MQ=255)
                           ttttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGc                                                                   <  1:2353812/99‑1 (MQ=255)
                              tCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGCTTAATTTCAGCGta                                                                >  2:1442475/1‑99 (MQ=255)
                                ccTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGtata                                                              >  2:157314/1‑99 (MQ=255)
                                ccTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGtata                                                              >  1:2449544/1‑99 (MQ=255)
                                ccTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTAGTATATTTTTTGAAACGCTGTTTTTTTTTCCTTTTGGATTAATTTCAGCGtata                                                              >  2:3850538/1‑99 (MQ=255)
                                 cTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAACGTGTATATTTTCTGAAACGCTGTTTTGTTTTCCTCTTGGATTAATTTCAGCGTATaa                                                             >  2:1043246/1‑99 (MQ=255)
                                  tGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAAt                                                            >  1:1591338/1‑99 (MQ=255)
                                    gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTATTTTCCTTTTGGATTAATTTCAGCGTATAATgc                                                          >  2:782601/1‑99 (MQ=255)
                                      aCGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgc                                                        <  2:3015289/99‑1 (MQ=255)
                                      aCGTTAAAACTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgc                                                        >  2:1741302/1‑99 (MQ=255)
                                       cGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGACACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcg                                                       <  2:836248/99‑1 (MQ=255)
                                       cGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcg                                                       <  1:1450425/99‑1 (MQ=255)
                                        gTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcgc                                                      >  2:2964152/1‑99 (MQ=255)
                                         ttAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGcc                                                     >  2:3357409/1‑99 (MQ=255)
                                           aaaTCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCaa                                                   <  1:1428034/99‑1 (MQ=255)
                                           aaaTCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCaa                                                   >  2:4250701/1‑99 (MQ=255)
                                             aTCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt                                                 <  2:2871802/99‑1 (MQ=255)
                                             aTCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt                                                 <  1:1493590/99‑1 (MQ=255)
                                             aTCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt                                                 <  1:1647140/99‑1 (MQ=255)
                                                 ttGATAACAATTTATTGTCTAAGAAGTTGTATATTTTTTGAAACGATGTTTTGTTTTCCTTTTGGATTAATTTAACCAGATATTGTGCGCTAATTAAct                                             >  2:3581924/1‑99 (MQ=255)
                                                 ttGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGAct                                             <  1:3030694/99‑1 (MQ=255)
                                                  tGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGActc                                            <  2:2885021/99‑1 (MQ=255)
                                                   gATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGActct                                           >  1:463183/1‑99 (MQ=255)
                                                   gATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGActct                                           <  1:2555266/99‑1 (MQ=255)
                                                    aTAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCtt                                          <  2:4172506/99‑1 (MQ=255)
                                                      aaCAATTTATTGTCTAACACGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGa                                        >  1:3393637/1‑99 (MQ=255)
                                                      aaCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGa                                        >  2:2326042/1‑99 (MQ=255)
                                                      aaCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTTTTTTGTTTTCCTTTTGGATTAATGTCAGCGTATAATGCGCGCCAATTGACTCTTGa                                        >  1:3913992/1‑99 (MQ=255)
                                                       aCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGaa                                       <  2:1746530/99‑1 (MQ=255)
                                                       aCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGaa                                       <  1:675831/99‑1 (MQ=255)
                                                        cAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt                                      <  1:6212/99‑1 (MQ=255)
                                                        cAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt                                      <  2:2029811/99‑1 (MQ=255)
                                                        cAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt                                      <  1:2254180/99‑1 (MQ=255)
                                                        cAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt                                      <  1:2062305/99‑1 (MQ=255)
                                                         aaTTTATTGTCTAACAAGTTGTATATTTTTTTTATTTTTTTTTTGTTTTCCTCTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAtc                                     >  2:1790262/1‑98 (MQ=255)
                                                           tttATTGTCTAACAAGTTGTATCTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGt                                   <  1:4025352/99‑1 (MQ=255)
                                                             tATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGttt                                 <  1:3614054/99‑1 (MQ=255)
                                                              aTTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTc                                <  1:3965218/99‑1 (MQ=255)
                                                               ttGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCa                               <  2:3760852/99‑1 (MQ=255)
                                                               ttGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCa                               <  2:1411297/99‑1 (MQ=255)
                                                               ttGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCa                               <  2:514419/99‑1 (MQ=255)
                                                                tGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAg                              <  1:3183369/99‑1 (MQ=255)
                                                                 gTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGc                             >  1:881356/1‑99 (MQ=255)
                                                                  tCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCa                            >  2:2739562/1‑99 (MQ=255)
                                                                    tAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACt                          >  1:816547/1‑99 (MQ=255)
                                                                      aCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACttt                        <  1:3055771/99‑1 (MQ=255)
                                                                      aCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACttt                        <  1:543802/99‑1 (MQ=255)
                                                                      aCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACttt                        >  1:2841680/1‑99 (MQ=255)
                                                                       cAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTg                       <  1:2741896/99‑1 (MQ=255)
                                                                         aGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGa                     <  1:3655520/99‑1 (MQ=255)
                                                                         aGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGa                     <  1:4212969/99‑1 (MQ=255)
                                                                         aGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGa                     <  1:777968/99‑1 (MQ=255)
                                                                         aGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGa                     >  1:2799116/1‑99 (MQ=255)
                                                                         aGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGa                     <  1:1620890/99‑1 (MQ=255)
                                                                          gTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGAc                    <  2:1953890/99‑1 (MQ=255)
                                                                           ttGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACt                   <  1:1940020/99‑1 (MQ=255)
                                                                            tGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTg                  >  2:2929703/1‑99 (MQ=255)
                                                                            tGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGCTTCAGCACTTTCGACTg                  <  1:3626796/99‑1 (MQ=255)
                                                                            tGTATATTTTTGAAACGCTGTTTTGTTTTACTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGt                 <  2:1323217/99‑1 (MQ=255)
                                                                              tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTa                <  1:1257903/99‑1 (MQ=255)
                                                                              tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTa                <  1:1104089/99‑1 (MQ=255)
                                                                              tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTa                <  2:1606376/99‑1 (MQ=255)
                                                                              tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTa                <  2:1470034/99‑1 (MQ=255)
                                                                                 atTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGaa             <  2:3460084/99‑1 (MQ=255)
                                                                                  ttttttGAAACTCTGGTTTGTTGGCCTGTTGGATTAATTTCGTGTTATAATGGGCGCCAGTTGACTCTTTAATGGTTTCAGCACTTTGGACTGTAGAAc            >  2:1258498/1‑99 (MQ=255)
                                                                                  ttttttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAAc            >  2:1652095/1‑99 (MQ=255)
                                                                                  ttttttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAAc            <  1:3165341/99‑1 (MQ=255)
                                                                                   tttttGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAAc            >  1:2940360/1‑99 (MQ=255)
                                                                                   tttttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCACCACTTTGGACTGTAGAACt           <  1:4328272/99‑1 (MQ=255)
                                                                                     tttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCa         >  2:182788/1‑99 (MQ=255)
                                                                                      ttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCaa        <  2:35978/99‑1 (MQ=255)
                                                                                       tGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAAc       <  2:1428508/99‑1 (MQ=255)
                                                                                       tGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAAc       <  1:2613726/99‑1 (MQ=255)
                                                                                       tGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAAc       <  1:1171188/99‑1 (MQ=255)
                                                                                       tGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAAc       <  2:3174425/99‑1 (MQ=255)
                                                                                          aaCGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGAc    >  2:1544195/1‑99 (MQ=255)
                                                                                           aCGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACt   <  2:57519/99‑1 (MQ=255)
                                                                                            cGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTc  <  2:504619/99‑1 (MQ=255)
                                                                                                 |                                                                                              
CGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTC  >  NC_000913/1755328‑1755519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: