Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913986,8480AG20.5% 467.7 / 99.5 224F45F (TTT→TTC) ompFouter membrane porin 1a (Ia;b;F)
Reads supporting (aligned to +/- strand):  ref base A (85/93);  new base G (31/15);  total (116/108)
Fisher's exact test for biased strand distribution p-value = 2.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 233 total aligned reads displayed.

GTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGC  >  NC_000913/986750‑986946
                                                                                                  |                                                                                                  
gTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGaa                                                                                                    <  1:1380706/99‑1 (MQ=255)
   aGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGaaaaa                                                                                                 <  1:3173742/99‑1 (MQ=255)
     aTCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAaata                                                                                               >  1:1985718/1‑99 (MQ=255)
     aTCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAaata                                                                                               >  1:1898054/1‑99 (MQ=255)
      tCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAaataa                                                                                              <  2:1160083/99‑1 (MQ=255)
      tCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAaataa                                                                                              <  1:2056852/99‑1 (MQ=255)
       cGGAATTGATTTGAGGTTCCCCTTAAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAaataat                                                                                             <  2:3518657/99‑1 (MQ=255)
           aTTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAg                                                                                         <  2:419457/99‑1 (MQ=255)
           aTTGATTTGAGTTTCACCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAg                                                                                         <  2:4079455/99‑1 (MQ=255)
                      tttCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCtt                                                                              >  2:1099503/1‑99 (MQ=255)
                        tCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTa                                                                            <  2:586432/99‑1 (MQ=255)
                         ccccTTTAAAACCAAGTCGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTAc                                                                           <  1:2586637/99‑1 (MQ=255)
                          cccTTTGCAACCAAGACGGGGATAAGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTAcc                                                                          <  2:1251629/99‑1 (MQ=255)
                          cccTTTAAAACCCAGCCGGGCATAGGTCATGTCGCCCTTGACACAGTAAATGTTTTCCACGTTACACTTGGAGCAATCATGCAGCCCGAACGCTTTAcc                                                                          >  1:3060379/1‑99 (MQ=255)
                          cccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                                                                          >  1:2742683/1‑99 (MQ=255)
                          cccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                                                                          >  1:1545154/1‑99 (MQ=255)
                          cccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                                                                          >  1:3253828/1‑99 (MQ=255)
                          cccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                                                                          >  1:2077854/1‑99 (MQ=255)
                          cccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                                                                          >  1:2176064/1‑99 (MQ=255)
                          cccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                                                                          >  1:2557030/1‑99 (MQ=255)
                          cccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTAcc                                                                          <  1:2399770/99‑1 (MQ=255)
                           ccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCg                                                                         >  1:23512/1‑99 (MQ=255)
                           ccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACAGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCg                                                                         >  1:4215205/1‑99 (MQ=255)
                            cTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGt                                                                        >  1:2357844/1‑99 (MQ=255)
                             tttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACTCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTa                                                                       >  1:536735/1‑99 (MQ=255)
                             tttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTa                                                                       <  1:3573103/99‑1 (MQ=255)
                             tttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTa                                                                       <  2:1682428/99‑1 (MQ=255)
                             tttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTAACATTAGAAAAATAATGCAGACCAACAGCTTTACCGTa                                                                       <  2:295562/99‑1 (MQ=255)
                              ttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTAc                                                                      >  1:2441412/1‑99 (MQ=255)
                                  aaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGAt                                                                  >  1:1565610/1‑99 (MQ=255)
                                  aaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGAt                                                                  <  2:1205892/99‑1 (MQ=255)
                                     cAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTa                                                               <  1:1771467/99‑1 (MQ=255)
                                           gggCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCGTGGAAAAATAATGCAGAACAACAGCTTTACCGTCCAGATCTACTttgt                                                         >  2:3867749/1‑99 (MQ=255)
                                            ggCATAGGTCATGTCGCCATTGCGACCGTAACTGTTTTCACCGTTACGCTTTGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTttgtt                                                        <  2:2129848/99‑1 (MQ=255)
                                            ggCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTttgtt                                                        <  1:826092/99‑1 (MQ=255)
                                            ggCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTttgtt                                                        <  1:2492671/99‑1 (MQ=255)
                                            ggCATACATCATGCAGCAATTGCCACCGAAACTGTTTTCACCGTTACCCTTTGAAAAAGAATGCAGACCAACAGCTTTACCGTACAGAGCTACTttgtt                                                        <  2:1960173/99‑1 (MQ=255)
                                             gCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTttgttg                                                       >  1:3014604/1‑99 (MQ=255)
                                             gCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTttgttg                                                       <  2:3048309/99‑1 (MQ=255)
                                                   gTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACGAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCt                                                 >  1:4047033/1‑99 (MQ=255)
                                                    tCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCtt                                                <  1:2126290/99‑1 (MQ=255)
                                                     cATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCttt                                               <  2:1770401/99‑1 (MQ=255)
                                                      aTGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTg                                              <  1:1466274/99‑1 (MQ=255)
                                                      aTGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTg                                              >  2:696519/1‑99 (MQ=255)
                                                      aTGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTg                                              >  2:1488684/1‑99 (MQ=255)
                                                       tGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGt                                             <  2:2186373/99‑1 (MQ=255)
                                                        gTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGtt                                            <  2:2496267/99‑1 (MQ=255)
                                                         tCGCCTTTGCCACCGAAAAAGAAGACACCGTAACCCGTGGCGAATTAATGCAGGCCTACAACTTTACCGTACAGAGCTACTTTGTTGCCATCTTTTTta                                           <  2:167103/99‑1 (MQ=12)
                                                         tCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTta                                           >  2:2325082/1‑99 (MQ=255)
                                                         tCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTta                                           >  1:139663/1‑99 (MQ=255)
                                                         tCGCAACTGCCACCGAAACTGTTTTCAGTGTTACCCTTGTAGAAATACTGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTGGCCATCTTTTTta                                           <  2:137191/99‑1 (MQ=21)
                                                          catcATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTtat                                          >  2:550156/4‑99 (MQ=255)
                                                          cGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTtat                                          >  1:2997354/1‑99 (MQ=255)
                                                          cGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTtat                                          <  1:2028577/99‑1 (MQ=255)
                                                           gCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTtata                                         <  2:2364788/99‑1 (MQ=255)
                                                           gCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTtata                                         >  1:34983/1‑99 (MQ=255)
                                                              aTTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATCGAt                                      <  2:3616924/99‑1 (MQ=38)
                                                               ttGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGAtt                                     >  2:3182827/1‑99 (MQ=255)
                                                                tGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGAttt                                    <  2:2049891/99‑1 (MQ=255)
                                                                tGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGAttt                                    <  2:441928/99‑1 (MQ=255)
                                                                  ccACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCt                                  <  1:281085/99‑1 (MQ=255)
                                                                  ccACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTCGTTGCCATCTTCGTTATAGATTTCt                                  <  2:3993751/99‑1 (MQ=255)
                                                                     ccGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCa                               <  1:262719/99‑1 (MQ=255)
                                                                      cGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGGACATATCTACTATGTTGCCATCGTTGTTATAGATTTCTGCAg                              >  2:3621352/1‑99 (MQ=255)
                                                                          tcTGTGTTTACCGTTACCCTGGGAAAAATAAAGCAGACTAACAGCTTTACCGTACAGATCTACTTTGTTGCCCCCCTTGTGATAGATTTCTGAAGCGtt                          >  2:485539/2‑99 (MQ=25)
                                                                           cTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGttt                         >  2:909139/1‑99 (MQ=255)
                                                                            tGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTg                        <  2:3250503/99‑1 (MQ=255)
                                                                            tGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTg                        <  2:1399666/99‑1 (MQ=255)
                                                                             gTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGc                       <  1:2705011/99‑1 (MQ=255)
                                                                             gTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGc                       <  2:2394053/99‑1 (MQ=255)
                                                                              ttttCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCa                      <  1:1077966/99‑1 (MQ=255)
                                                                              ttttCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCa                      >  2:1581302/1‑99 (MQ=255)
                                                                              tttaCACCGTTACCCTTGGAACAATCGTGTTGACTAACAGCTTTAGCGTACAGATCTACTTTGTTGCCATCTTCGTTATAGTTTTCTGCAGCGTGTGCa                      <  2:868966/99‑1 (MQ=255)
                                                                               tttCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAg                     >  1:1180421/1‑99 (MQ=255)
                                                                                ttCACCGTTACTCTTGGAAAAATAATGAAGACCAACAGGTGTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGt                    <  2:2547048/99‑1 (MQ=255)
                                                                                ttCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGt                    >  2:338714/1‑99 (MQ=255)
                                                                                 tCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTa                   >  1:597745/1‑99 (MQ=255)
                                                                                  cACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTAc                  <  2:2596734/99‑1 (MQ=255)
                                                                                  cACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTAc                  <  2:3769120/99‑1 (MQ=255)
                                                                                   aCCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTAcc                 >  1:1267051/1‑99 (MQ=255)
                                                                                   aCCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTAcc                 >  1:1069727/1‑99 (MQ=255)
                                                                                   aCCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTAcc                 <  1:4169504/99‑1 (MQ=255)
                                                                                     cGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTg               >  1:3502639/1‑99 (MQ=255)
                                                                                      gTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGc              <  2:2252707/99‑1 (MQ=255)
                                                                                       ttACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGct             >  1:2425310/1‑99 (MQ=255)
                                                                                           ccTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGctacta         >  1:981922/1‑99 (MQ=255)
                                                                                           ccTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGctacta         >  2:3097894/1‑99 (MQ=255)
                                                                                           ccTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGctacta         >  1:3722038/1‑99 (MQ=255)
                                                                                            cTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTaa        >  1:4235740/1‑99 (MQ=255)
                                                                                            cTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTaa        >  1:1570766/1‑99 (MQ=255)
                                                                                            attGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCAGCTACTaa        >  1:1787302/2‑99 (MQ=25)
                                                                                             ttGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAAc       >  1:2292189/1‑99 (MQ=255)
                                                                                             ttGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAAc       >  2:95209/1‑99 (MQ=255)
                                                                                             ttGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAAc       >  2:3037606/1‑99 (MQ=255)
                                                                                              tGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACa      <  2:34532/99‑1 (MQ=255)
                                                                                              tGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACa      <  1:595123/99‑1 (MQ=255)
                                                                                               ggAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACag     >  1:3887966/1‑99 (MQ=255)
                                                                                               ggAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACag     >  1:177746/1‑99 (MQ=255)
                                                                                                gAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACaga    <  1:2262794/99‑1 (MQ=255)
                                                                                                 aaaaaTAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACagag   >  1:3211678/1‑99 (MQ=255)
                                                                                                  |                                                                                                  
GTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGC  >  NC_000913/986750‑986946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑