Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,533,492 A→T intergenic (+112/‑272) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,533,4920AT97.7% 580.8 / 3.9 171intergenic (+112/‑272)cysK/ptsHcysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (82/84);  total (84/87)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 172 total aligned reads displayed.

AATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGG  >  NC_000913/2533396‑2533587
                                                                                                |                                                                                               
aaTGCGTAAAAAAGCACCTTTTTAGGTACTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTAc                                                                                               <  1:743458/99‑1 (MQ=255)
   gCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAg                                                                                            >  1:3587932/1‑99 (MQ=255)
    cGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGa                                                                                           <  2:3824136/99‑1 (MQ=255)
    cGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGa                                                                                           >  2:1099768/1‑99 (MQ=255)
    cGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGa                                                                                           <  1:2836206/99‑1 (MQ=255)
     gTAAAAGAGCACCCTGTCAGGTGCGTTTTGGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATGGATTCATCCTGTCGGAACTAGTATTTTACCAGAc                                                                                          <  2:1461097/99‑1 (MQ=255)
     gTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGAc                                                                                          >  2:888964/1‑99 (MQ=255)
     gTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACt                                                                                         >  2:3033856/1‑99 (MQ=255)
      tAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACt                                                                                         >  2:2742060/1‑99 (MQ=255)
       aaaaaaGCAGCTTTTTAGGTGCTTTTTTGTGGCATGCTTCAAACTTTCGCCCGTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTa                                                                                        >  2:3942899/1‑99 (MQ=255)
       aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCTAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTa                                                                                        >  1:4132586/1‑99 (MQ=255)
       aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTa                                                                                        >  1:1096343/1‑99 (MQ=255)
         aaaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAat                                                                                      <  2:1676918/99‑1 (MQ=255)
          aaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAatt                                                                                     >  2:3798610/1‑99 (MQ=255)
          aaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAatt                                                                                     >  1:2395575/1‑99 (MQ=255)
           aaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCTGCCTGTCGGAACTGGTATTTTACCAGACTAatta                                                                                    >  1:3908492/1‑99 (MQ=255)
             gCACCTTTTTAGGAGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAattatt                                                                                  <  2:1191977/99‑1 (MQ=255)
              cACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCACACTTTCCCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTAttt                                                                                 <  2:4075070/99‑1 (MQ=255)
               aCCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTAtttt                                                                                <  1:1967451/99‑1 (MQ=255)
               aCCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTAtttt                                                                                >  1:1832222/1‑99 (MQ=255)
               aCCTTTTTAGGTGCTTTTATGTGACCTGCTTCAAACTTTCCCACCTCCTCCCATACACTCAGCCTGTCGGCACTCGTCCTTCCCCAGCCTAAATAattc                                                                                >  2:411415/1‑98 (MQ=255)
                ccTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTg                                                                               <  1:788631/99‑1 (MQ=255)
                  tttttAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGAt                                                                             <  1:250374/99‑1 (MQ=255)
                  tttttAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGAt                                                                             <  1:3282656/99‑1 (MQ=255)
                  tttttAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGAt                                                                             <  2:1813150/99‑1 (MQ=255)
                    tttAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATgc                                                                           <  1:478046/99‑1 (MQ=255)
                      tAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATATTACCAGACTAATTATTTTGATgcgc                                                                         <  2:4254901/99‑1 (MQ=255)
                       aGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGcgcg                                                                        >  1:1873183/1‑99 (MQ=255)
                        ggTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGa                                                                       <  1:2117244/99‑1 (MQ=255)
                          tGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGaaa                                                                     >  2:1653307/1‑99 (MQ=255)
                               ttttGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAAt                                                                <  1:3088447/99‑1 (MQ=255)
                               ttttGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAAt                                                                <  2:2549782/99‑1 (MQ=255)
                                tttGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATc                                                               <  2:105004/99‑1 (MQ=255)
                                 ttGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCg                                                              <  2:1485121/99‑1 (MQ=255)
                                 ttGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCg                                                              <  1:229980/99‑1 (MQ=255)
                                  tgtgGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGt                                                             <  1:47131/99‑1 (MQ=255)
                                    tgGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTa                                                           <  2:3505953/99‑1 (MQ=255)
                                     ggCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTAc                                                          <  1:1212773/99‑1 (MQ=255)
                                      gCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACa                                                         <  2:2997292/99‑1 (MQ=255)
                                      gCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACa                                                         >  1:256342/1‑99 (MQ=255)
                                      gCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACa                                                         >  1:3038441/1‑99 (MQ=255)
                                      gCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACa                                                         <  1:3995370/99‑1 (MQ=255)
                                        cTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAgg                                                       >  1:874490/1‑99 (MQ=255)
                                        cTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAgg                                                       >  1:2415533/1‑99 (MQ=255)
                                         tGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGa                                                      <  1:2841900/99‑1 (MQ=255)
                                           cTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACAAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGaaa                                                    >  2:1325547/1‑99 (MQ=255)
                                            ttCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGaaaa                                                   <  2:1936691/99‑1 (MQ=255)
                                            ttCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGaaaa                                                   <  2:2128401/99‑1 (MQ=255)
                                            ttCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGaaaa                                                   >  1:1433865/1‑99 (MQ=255)
                                             tCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAg                                                  <  1:1698166/99‑1 (MQ=255)
                                              cacaCTTTAGCCCCTCCTGGCATTGATTAAGCCTGTCGGAAATGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGc                                                 <  1:2378889/96‑1 (MQ=255)
                                               aaaCTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGCCTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGcc                                                >  2:137910/1‑99 (MQ=255)
                                               aaaCTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGcc                                                <  1:3976940/99‑1 (MQ=255)
                                               aaaCTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGcc                                                >  2:690814/1‑99 (MQ=255)
                                                aaCTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCa                                               >  2:583827/1‑99 (MQ=255)
                                                 aCTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCaa                                              >  2:792033/1‑99 (MQ=255)
                                                   tttCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAg                                            >  1:515967/1‑99 (MQ=255)
                                                    ttCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGc                                           <  1:2025993/99‑1 (MQ=255)
                                                       gCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGa                                        >  1:2398695/1‑99 (MQ=255)
                                                        ccccTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGaa                                       >  2:1310706/1‑99 (MQ=255)
                                                           ctcctGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa                                   <  1:3072916/99‑1 (MQ=255)
                                                             cctGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAt                                  <  2:3147139/99‑1 (MQ=255)
                                                              ctGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtt                                 <  1:4033027/99‑1 (MQ=255)
                                                              ctGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtt                                 <  2:67024/99‑1 (MQ=255)
                                                               tGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAttt                                <  2:2868781/99‑1 (MQ=255)
                                                                 gCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtgcca                              >  2:2304640/1‑95 (MQ=255)
                                                                 gCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTa                              <  1:2314110/99‑1 (MQ=255)
                                                                   aTTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg                            >  1:2946336/1‑99 (MQ=255)
                                                                   aTTGATTCAGCCAGTCGGAACTGGTACATTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg                            >  1:3824576/1‑99 (MQ=255)
                                                                     tGATTCAGCCTTTCGGAACTGGTATTTTACCATACTAATTATTTTGAGGCGCGAAATTAAGCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAt                          <  1:4139025/99‑1 (MQ=255)
                                                                      gATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAtt                         <  1:850359/99‑1 (MQ=255)
                                                                       aTTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAttt                        >  2:393218/1‑99 (MQ=255)
                                                                       aTTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAttt                        <  2:3520430/99‑1 (MQ=255)
                                                                       aTTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAttt                        >  2:3325125/1‑99 (MQ=255)
                                                                       aTTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAttt                        >  1:2634927/1‑99 (MQ=255)
                                                                         tCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTgg                      >  2:3809293/1‑99 (MQ=255)
                                                                           aGCCTGTCGGCACTGGTATTGTACCTGACTAATTATTTTGATGCGTGAAATTAATCGTTACATGAAATGCCAAAGCTGAATCGATTTTATGCTTTGGtt                    <  2:2169727/99‑1 (MQ=255)
                                                                           aGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGtt                    <  1:1049027/99‑1 (MQ=255)
                                                                           aGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGtt                    >  2:1857444/1‑99 (MQ=255)
                                                                            gCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTc                   >  2:1996553/1‑99 (MQ=255)
                                                                            gCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGACTTGGTTc                   >  2:1996554/1‑99 (MQ=255)
                                                                             ccTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCa                  >  1:4230561/1‑99 (MQ=255)
                                                                              cTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCaa                 <  2:2174096/99‑1 (MQ=255)
                                                                              cTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCaa                 >  2:2083931/1‑99 (MQ=255)
                                                                               tGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCact                >  1:3517772/1‑97 (MQ=255)
                                                                               tGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAt                <  2:4033557/99‑1 (MQ=255)
                                                                                gTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAtt               >  1:1779676/1‑99 (MQ=255)
                                                                                gTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAtt               >  1:1533585/1‑99 (MQ=255)
                                                                                   ggAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAttctt            >  2:3710666/1‑99 (MQ=255)
                                                                                     aaCTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTcc          <  1:4308104/99‑1 (MQ=255)
                                                                                      aCTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCt         <  1:1994572/99‑1 (MQ=255)
                                                                                      aCTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCt         >  2:3070351/1‑99 (MQ=255)
                                                                                        tGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCttt       >  1:3449709/1‑99 (MQ=255)
                                                                                        tGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCATCCttt       >  2:1047099/1‑99 (MQ=255)
                                                                                         ggTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTa      <  2:3875932/99‑1 (MQ=255)
                                                                                         ggTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTa      >  1:3147999/1‑99 (MQ=255)
                                                                                          gTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAg     >  1:1501826/1‑99 (MQ=255)
                                                                                          gTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAg     >  1:2744399/1‑99 (MQ=255)
                                                                                          gTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTGGGTTCAATTCTTCCTTTAg     <  1:3394486/99‑1 (MQ=255)
                                                                                             ttttACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCgg  >  1:2803008/1‑99 (MQ=255)
                                                                                                |                                                                                               
AATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGG  >  NC_000913/2533396‑2533587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: