Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,1250TA35.9% 70.4 / 21.4 39intergenic (‑330/+60)ybgD/gltAputative fimbrial‑like adhesin protein/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (25/0);  new base A (0/14);  total (25/14)
Fisher's exact test for biased strand distribution p-value = 6.63e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATC  >  NC_000913/752898‑753353
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    
attaAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCatat                                                                                                                                                                                                                                      >  1:1769302/1‑228 (MQ=255)
attaAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCAtata                                                                                                                                                                                                                                     <  1:1263860/229‑1 (MQ=255)
                                                            gggggTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTAAAAATTAAAAAACAAACAtt                                                                                                                                                                         >  1:1129707/1‑229 (MQ=255)
                                                                 tataGATAGACTTTTTTTTAATGGGTTTATAAAGGAGATT‑ACAATGAATTTTTTAATTAAAATTTTTGGTTTGCTAATATTTTGAGTTTTTTTTTTTTTTTTAAATATTTATATAGGTTTTTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGAAAAAAAAAAAAAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                                                                                                                     <  2:1741819/212‑1 (MQ=255)
                                                                 tataGATAGACGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTCAAATTaa                                                                                                                                                                                     >  1:1744792/1‑211 (MQ=255)
                                                                 tataGATAGACGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAACTTAGAAATCAACCCTTAAc                                                                                                                                                                      >  1:143715/1‑227 (MQ=255)
                                                                         taatgaaTTTTCAGAGGGTTATAAATGC‑ACTGACGAAGTGTTTTTTAATAAAAATTTTTGGGTTGTAGATATTTTGTGCGTTTATGTTTTTTTTAAATATCTATAAGGTTCGGTAAATGTTTTTTATTTTTAGATTTTTTTGTTTTTGGGCAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                                                                                                             <  2:1108795/222‑1 (MQ=255)
                                                                           cGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAAcg                                                                                                                                                                                                              >  1:401994/1‑177 (MQ=255)
                                                                                                   gCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAccc                                                                                                                                                                                                                          >  1:1936785/1‑141 (MQ=255)
                                                                                                   gCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCAGCCATATGAACGGCGGGTTAAAAAATTTTCAACTTAGAAATCAACAACTAACGCTTGATATCGCGTTTAAAATCGCGTTTTTCatat                                                                                                                                   >  2:1930453/1‑228 (MQ=255)
                                                                                                     gACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTCAACTTATCAAACAACCATTAACGCTTGATATCGCTTTTTAAGTCGTGttttttctat                                                                                                                                   >  2:1065879/1‑221 (MQ=255)
                                                                                                                     tttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTCAAATTTACAAACAACCATTAACACTTGATTTTGCTTTTTAAGATGCGTTTTTCATATTCTTtataaatattacgc                                                                                                                 >  2:1086782/1‑219 (MQ=255)
                                                                                                                             aaaGTATGGGGTTGTTTATATTTTGATTTTTTTTTTTTTTTTTAAATATTTATATAGGTCTGTTAATGGTTTTTATTTTTACATTTTTTTGTGTTTAGGAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                                                                                                         <  1:1067756/229‑1 (MQ=255)
                                                                                                                              aaGTATTGGTTTTATTATTATTGGATTTTTTTTATTTTTTTAAAATTTTTATTTAGTTATTTAAATGGTTTTTTTTTTTAAAATTTTTGTTGTTTGACAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                                                                                                         <  2:1856274/228‑1 (MQ=255)
                                                                                                                                    tGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAACTTAGCAAACAACCATTAACGCCTGATATCGCTTTTAAAGTCGCGGTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                                                                                                         >  1:1859440/1‑222 (MQ=255)
                                                                                                                                                                     tttAAATATCTATATGGGTCTGTTAATGTTTTTTATTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                                                                                                            <  1:1732199/154‑1 (MQ=255)
                                                                                                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTAAAACTTAGCa                                                                                                                                                                                   >  2:2462/1‑115 (MQ=255)
                                                                                                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTCAACTTAGCAATCAACCATTAAAGCTTGATATCGCTTTTTAAGTCGCGTTTTTTATATCCTGTATACAGCTTACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCTCTCCacggggg                                                                  >  2:678441/1‑222 (MQ=255)
                                                                                                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTATAAATTAGCAATCAACCATTAAACCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTTTATAAAACTGAAGCGGGCGGGGGATCTTCATACCCTCCCTTTGCATTTCTCTCCCGTgggg                                                                  >  2:1291857/1‑227 (MQ=255)
                                                                                                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCCTTTAAACTcgccg                                                                                                                                            >  2:1729245/1‑152 (MQ=255)
                                                                                                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTAAAACTTAGAAAt                                                                                                                                                                                 >  1:303789/1‑117 (MQ=255)
                                                                                                                                                                     ttaaaaTATCTATAAAGTTTTGTTAATGGTTTTAATTTTAACAATTTTTTGTGTTTAGGAATAAAAAAACAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCa                                                                                                                                                                                   <  1:2467/112‑1 (MQ=255)
                                                                                                                                                                      taaaaTATCTATAGAGGTCTTTAAAGGGTTTTTTTTTTTACAATTTTTTGTTTTTAGGCAAAAAAAACACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCg                                                                 <  1:1294097/226‑1 (MQ=255)
                                                                                                                                                                              ttaaataGGTTTGTTAATGTATTTTTTTTTTCAAATTTTTTTTTTTAAGGAATAAAAAAACAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCa                                                        <  1:1088690/225‑1 (MQ=255)
                                                                                                                                                                                      tttttttAATGATTTTTTTTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCa                                                                                                                                                                               <  1:484310/97‑1 (MQ=255)
                                                                                                                                                                                      ttCTGTTAATGGATTTTATTTTTACATTTTTTTTTTTTAAGGCATAAAAAAACCACCCCGCCATATGAAcggc                                                                                                                                                                                                            <  2:741384/72‑1 (MQ=255)
                                                                                                                                                                                      gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCaa                                                                                                                                                                                                                             >  1:448228/1‑56 (MQ=255)
                                                                                                                                                                                      gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAAcggc                                                                                                                                                                                                            >  1:742684/1‑73 (MQ=255)
                                                                                                                                                                                      gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAAcgg                                                                                                                                                                                                             >  1:1782505/1‑72 (MQ=255)
                                                                                                                                                                                      gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTATAAATTAGAAATCa                                                                                                                                                                               >  2:483454/1‑102 (MQ=255)
                                                                                                                                                                                         ttttAATGGATTTTTTTTTTACATTTTTTTGTGTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTa                                                                                                                                                                                             <  1:1606159/83‑1 (MQ=255)
                                                                                                                                                                                         tttaattGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGACATAAAAAAACAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCgg                                                                                                               <  1:1837511/157‑1 (MQ=255)
                                                                                                                                                                                         tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTAAACTTTGCAATCAACCATTAACGCTTGATATTGCTTTTTAAGTCGCGTTTTTTATATCCTGTATAAAACTGACGCgg                                                                                                               >  2:1834387/1‑163 (MQ=255)
                                                                                                                                                                                         tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAAAATTTa                                                                                                                                                                                             >  2:1603427/1‑85 (MQ=255)
                                                                                                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTATAAAATACAAATCAACCATTAAACGATGAGATCGCTTTTTAAATcgg                                                                                                                                              >  2:123427/1‑129 (MQ=255)
                                                                                                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAAAATTTACAACTTAACAATCAACCATTAACACTTGATATAGCTTTTAAAATCGCGTTTTTCATATCCTGTATACATCTGACGCGGACGGGCAATCTTCATACCATCACTGTGCCTTTTCCTCCAGTGAGCGATCCAACCAAAGGCACGtttca                                           >  2:1701781/1‑225 (MQ=255)
                                                                                                                                                                                           taaaTGGTTTTTTTTTTTACATTTTTTTGTGTTGAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTcac                                                                                                                                              <  1:123633/128‑3 (MQ=255)
                                                                                                                                                                                                     ttattttTACAATTTTTTTTGTTTAGGAAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGcc                                                         <  1:1649303/205‑1 (MQ=255)
                                                                                                                                                                                                     ttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGAAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTTTTCATTTCGCTCCAGTGGGCGGTCCAGcc                                                         >  2:1646497/1‑205 (MQ=255)
                                                                                                                                                                                                                aTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATTAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                                                                                                                     >  1:283929/1‑118 (MQ=255)
                                                                                                                                                                                                                             aGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTAAAACTTAGCAATCAAACATTAAAGCTTGATATCGCTTTTAAAAACGCGTTTTTTATATCCCGTATTAAGCTGAACCGGAAGGGCAATCGTCATATCGTCCCCTTGCATTTCGCTCCAGTGGGTGATCCAGCCAAAGGCAAGGTCCGTTGCGAAAAAGAAGGTGAGAAATGAAGACGGCAt         >  2:339315/1‑229 (MQ=255)
                                                                                                                                                                                                                                   ataAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCa    <  1:178886/228‑1 (MQ=255)
                                                                                                                                                                                                                                    taAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGGGAACATGGAAGACGGAATACCCATc  >  1:1057582/1‑229 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    
ATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACT‑ACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATC  >  NC_000913/752898‑753353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑